24562-PIERCE,Pierce Glycoprotein Staining Kit-琼脂糖

发表于由

【简单介绍】

货号：24562，名称： Pierce Glycoprotein Staining Kit ，品牌：PIERCE，规格：KIT

【详细说明】

订货：，，

 

Pierce Glycoprotein Staining  Kit介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

Pierce Glycoprotein Staining  Kit现货供应

 

PIERCE

24562

Pierce Glycoprotein Staining  Kit

KIT

PIERCE

24590

Gelcode Blue Stain Reagent,   500 Ml. （see also #24592-3.5L）

500 ML

PIERCE

24596

GelCode（tm） Blue Safe Protein Stain, 3.5 L.

3.5 LITER

 

Pierce Glycoprotein Staining  Kit品牌介绍

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括：

1）化学发光体和比色底物

 

2）分析试剂

 

3）荧光标记探针

 

4）酶联免疫分析试剂盒等

 

Pierce Glycoprotein Staining  Kit仅用于科研

 

The Thermo Scientific Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff （PAS） method.

The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins （glycoproteins） that have been separated by polyacrylamide gel electrophoresis （PAGE）. A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff （PAS） reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

Highlights:

  • Specific for glycoproteins – periodic acid-Schiff （PAS） method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
  • Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours （half the time required for other staining methods）
  • Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background
  • Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16µg horseradish peroxidase per band
  • Controls included – complete kit includes positive and negative control proteins （HRP and STI, respectively）.
  • Gels or membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes