F400Z-Cygnus Protein A ELISA Kit试剂盒-ELISA检测试剂盒

Cygnus Protein A ELISA Kit试剂盒
规格型号:96T/盒

Cygnus Protein A ELISA Kit试剂盒 产品介绍:

新的蛋白A ELISA试剂盒设计可克服许多商业试剂盒检测分析蛋白A回收低的困难。大多数低回收的样品要么是不完整的抗体片断或所谓的融合蛋白,其抗体结构与非抗体分子连接。非天然药物结构意味着传统样品处理程序在分离、变性、沉淀及蛋白A检测前的药品清除过程中没有效果。处理后样品中残留的非变性药品可与蛋白A再聚集,可用ELISA回收。

F400Z蛋白A ELISA试剂盒已使用几种未知样品鉴定,可全部回收蛋白A。这种试剂盒与通用的F400试剂盒使用相同的抗体、标准品和样品处理方法。*不同的是F400Z包含“Recovery Enhancer”溶液,使用前必须与HRP标记的抗蛋白A抗体(F401Z)结合。“Recovery Enhancer”可阻止在样品处理过程中没有完全去除的任何其它药物的再结合。

F400Z试剂盒可以准确定量非天然结构的蛋白A以及其它保守的、天然的和重组的蛋白A结构物。由于不是所有药物在样品处理过程中表现相同,因此必需在报道出结果前确认每个新的样品类型和用于分析回收的药物。

蛋白A可固定于不同的层析介质上,通常用于纯化抗体。共价结合后蛋白A可从色谱载体上过滤掉,与结合的抗体一起洗脱出来。治疗用抗体如果污染有蛋白A必须减少以避免任何副作用。这种免疫检测方法检测蛋白A污染灵敏度<100 pg/ml。过滤的蛋白A通常通过Fc区结合到样品的产物免疫球蛋白。蛋白A结合到产物抗体在一些免疫检测中可干扰蛋白A的精确定量,是因为其抑制了检测中结合到混合蛋白A的抗蛋白A抗体活性,会降低检测到的污染蛋白A量。

 

注意:Cygnus Protein A ELISA Kit检测试剂盒只用于研究或工业生产,终止剂为0.5 M H2SO4,使用时做好防护措施。具体使用方法请严格参照说明书。

This NEW PROTEIN A ELISA kit is designed to overcome low analytical recovery of Protein A encountered in many commercial kits. Most low recovery samples are either incomplete antibody fragments or so-called fusion proteins where the antibody structure is linked to another non-antibody molecule. The properties of such unnatural drug substance constructs, means that conventional sample treatment procedures are ineffective in dissociation, denaturation, precipitation, and removal of the drug substances prior to the Protein A detection assay. Any un-denatured drug substance remaining in the sample after the treatment step can reassociate with leached Protein A, resulting in under recovery by ELISA.

The #F400Z  Protein A ELISA kit has been qualified using several problematic samples and found to yield acceptable recovery on all. This kits uses the same antibodies, standards and sample treatment protocol as in our widely used #F400 kit. The only difference is that the #F400Z kit contains a “Recovery Enhancer” solution which must be combined with the HRP conjugated anti-Protein A antibody, #F401Z, prior to use. The “Recovery Enhancer” prevents the re-association of any drug substance that was not compley removed during the sample treatment step. 

The #F400Z kit will accuray quantitate the unnatural construct of Protein A, MabSelect SuRe™ as well as other conserved, natural and recombinant Protein A constructs. Because not all drug substances will respond equally to sample treatment procedures, your lab must validate each new sample type and drug substance for acceptable analytical recovery before reporting results.