遗传霉素

遗传霉素

货号:
IG0010

品牌:
Jinpan

遗传霉素

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产品简介
EC EINECS 600-864-4
MDL MFCD00058314
别名 G 418 disulfate salt; G418 Sulfate
英文名称 G-418 Sulfate
CAS 108321-42-2
分子式 C20H44N4O18S2
分子量 692.71
纯度 ≥98%
单位
生物活性 G-418 disulfate 是与 gentamicin B1 的结构相似的氨基糖苷类抗生素,在原核和真核细胞的蛋白质合成过程中,能够阻止蛋白质的延伸阶段,从而抑制蛋白质的合成[1-3]。
In Vitro G418是许多原核和真核生物的抑制剂,浓度为1-300微克/毫升。由编码氨基糖苷类3′-磷酸转移酶的Tn5的neo基因对G418的抗性,APT 3’II通常用于实验室研究以选择基因工程细胞[1]。通常,对于细菌和藻类,使用5mg/L或更低的浓度,对于哺乳动物细胞,使用约400mg/L的浓度进行选择,使用200mg/L进行维持。抗性克隆的选择可能需要1到3周[2]。
In Vivo 连续三天G418(40和80 mg/kg)足以消除感染小鼠中所有未转染的布氏疱疹病毒寄生虫[3]。
SMILES O[C@@H]1[C@@H]([C@H](O)C)O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@@H]3[C@H](O)[C@@H](NC)[C@@](C)(O)CO3)[C@H](N)C[C@@H]2N)[C@H](N)[C@H]1O.O=S(O)(O)=O.O=S(O)(O)=O
靶点 Bacterial
动物实验 为了表征锥虫群体对G418体内的敏感性,布氏布鲁氏菌GUTat 3.1和布氏布鲁氏菌GUTat 3.1/BBR3的血流形式在亚致死辐射的小鼠中分别扩增。在寄生虫血症的第一个峰之前,收集锥虫,并将含有106个锥虫的等分试样腹膜内接种到小鼠中。感染后24小时,将小鼠分成组,并通过腹膜内接种0.2mL药物,以10,20,30,40,50或80mg/kg体重(bw)的剂量用G418处理。在无菌水中。在第一次治疗后24和48小时,以与之前相同的剂量向每组动物施用G418,导致每只小鼠进行三次处理。需要重复药物治疗以确保从小鼠中完全消除未转染的GUTat 3.1寄生虫。然后通过显微镜检查湿血膜每天监测小鼠33天,以检测寄生虫的存在。记录发现寄生虫的动物,然后从实验中取出。
数据来源文献 [1]. Davies J, et al. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg. 1980 Sep;29(5 Suppl):1089-92.
[2]. Li Y, et al. Inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma cells in vitro. World J Gastroenterol. 2003 Oct;9(10):2174-7.
[3]. Murphy NB, et al. Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants. Antimicrob Agents Chemother. 1993 May;37(5):1167-70.
规格 100mg 500mg 10mM*1mL (in Water) 1g

是一种氨基糖苷类抗生素,是稳定转染最常用的抗性筛选试剂。通过抑制转座子Tn601,Tn5的基因,干扰核糖体功能而阻断蛋白质合成,对原核和真核等细胞产生毒素。当neo基因被整合进真核细胞DNA后,则能启动neo基因编码的序列转录为mRNA,从而获得抗性产物氨基糖苷磷酸转移酶的高效表达,使细胞获得抗性而能在含有G418的选择性培养基中生长。

使用本产品的应用案例(仅供参考

In Vitro

Cell(HepG2.2.15 cells,380 µg/mL G-418 sulfate

Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Jinpan) and supplemented with 10% fetal bovine serum (FBS).G-418 sulfate (380 µg/mL; Jinpan) was added to DMEM with 10% FBS to maintain HepG2.2.15 cells. All cells were incubated at 37°C with 5% CO2.

来源文献:Guo C, Ouyang Y, Cai J, Xiong L, Chen Y, Zeng X, Liu A. High expression of IL-4R enhances proliferation and invasion of hepatocellular carcinoma cells. Int J Biol Markers. 2017 Oct 31;32(4):e384-e390. doi: 10.5301/ijbm.5000280. PMID: 28665449.

Cell((HepG2.2.15 cells,300 µg/mL G-418 )

HepG2.2.15 cells were incubated in minimum Eagle’s medium containing 10% FBS and 300 µg/mL G418 (Jinpan, Beijing, China). 

来源文献:Jiang W, Wang L, Zhang Y, Li H. Circ-ATP5H Induces Hepatitis B Virus Replication and Expression by Regulating miR-138-5p/TNFAIP3 Axis. Cancer Manag Res. 2020 Nov 2;12:11031-11040. doi: 10.2147/CMAR.S272983. PMID: 33173336; PMCID: PMC7648158.


Cell(OC cells,800 μg/mL  G418

PCNP expression plasmid and empty vector, the PCNP shRNA (sh-PCNP group) and scramble shRNA (sh-Scb group) and were transfected into OC cells with Lipofectamine 3000 Transfection Reagent to construct stable cell lines. They were screened, respectively, by G418 (Jinpan, Shanghai, China) at a concentration  of 800 μg/mL and puromycin (Jinpan, Shanghai, China) at a concentration of 2 μg/mL. 

来源文献:Dong P, Fu H, Chen L, Zhang S, Zhang X, Li H, Wu D, Ji X. PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition. J Cell Mol Med. 2020 Jul;24(14):8221-8235. doi: 10.1111/jcmm.15491. Epub 2020 Jun 16. PMID: 32548978; PMCID: PMC7348179.

Cell(Replicon cell ,500 μg/ml G418

Replicon cell lines were selected and maintained in 500 μg/ml G418 (Geneticin; Jinpan, China). 

来源文献:Sobhanimonfared F, Bamdad T, Roohvand F. Cross talk between alcohol-induced oxidative stress and HCV replication. Arch Microbiol. 2020 Sep;202(7):1889-1898. doi: 10.1007/s00203-020-01909-9. Epub 2020 May 24. PMID: 32448963.