High purity Cellulase (endo-1,4-β-D-glucanase) (T. Longibrachiatum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.4

From Trichoderma longibrachiatum. Electrophoretically homogeneous. 
In 3.2 M ammonium sulphate.

Specific activity: ca. ~ 70 U/mg (40^oC, pH 4.5, CM-cellulose 4M as substrate). 

Stable at 4^oC for > 4 years.

(乙酰-CoA合成酶)(1)(柠檬酸合成酶)(2)2(L-苹果酸脱氢酶)+170.5 mL 配好的试剂 (R1 + R2)分光光度计，340 nm~15min10mg/L(使用推荐方法)葡萄酒、啤酒、水果和果汁、软饮料、醋、蔬菜、泡菜乳制品（如奶酪）、肉、鱼、面包、焙烤食品（和发酵粉）、番茄酱、酱油、蛋黄酱、调味汁、纸（硬纸板）、茶、医药品（如注射液）、饲料和其他原料（生物培养基、样品等）该实验方法已通过EN、ISO、ICUMSA、德国和荷兰的认证           

优点：

不会浪费ACS溶液（提供的  为稳定的悬浮液）加入PVP防止丹宁酸的抑制自动分析检测时配置好的试剂非常稳定    (> 5天， 4摄氏度)

最终反应液中的乙酸线性高达已通过法国葡萄酒大学验证

价格低廉     (每ml试剂的成本)所有试剂配制后的稳定性      >2年

Analyser format for the specific assay of acetic acid (acetate) in beverages and food products. Content:141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL)

Analyser format UV-method for the determination of Acetic Acid 
in foodstuffs, beverages and other materials

Principle:
                           (acetyl-CoA synthetase)
(1) Acetic acid + ATP + CoA → acetyl-CoA + AMP + pyrophosphate

                                                 (citrate synthase)
(2) Acetyl-CoA + oxaloacetate + H₂O → citrate + CoA

                (L-malate dehydrogenase)
(3) L-Malate + NAD⁺ ↔ oxaloacetate + NADH + H⁺

Kit size:                           141.6 mL of prepared reagent (R1 + R2) 
Method:                           Spectrophotometric at 340 nm
Reaction time:                 ~ 15 min
Detection limit:                10 mg/L (recommended assay format)
Application examples: 
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, 
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products 
(and baking agents), ketchup, soy sauce, mayonnaise, dressings, 
paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), 
feed and other materials (e.g. biological cultures, samples, etc.) 
Method recognition:     
Methods based on this principle have been accepted by EN, ISO, 
ICUMSA, IFU and MEBAK

Advantages

• No wasted ACS solution (stable suspension supplied)
   
• PVP incorporated to prevent tannin inhibition
   
• Very stable reagent when prepared for auto-analyser applications (> 3 days at 4°C)
   
• Linear calibration up to 30 μg/mL of acetic acid in final reaction solution
   
• Validated by the University of Wine, Suze la Rousse, France
   
• Very competitive price (cost per mL of reagent)
   
• All reagents stable for > 2 years after preparation

分析物意义：许多豆科植物的储备糖 

Megazyme检测试剂盒优点：只有试剂盒可用，试剂稳定，功能强大。AOAC方法2002.02；AACC方法32-40. 

The Galactomannon test kit is suitable for the measurement and analysis of Galactomannan in food and plant products.

UV-method for the determination of Galactomannan in legume
seeds, foodstuffs and plant products

Principle:
(β-mannanase)
(1) Galactomannan + H₂O → galactomanno-oligomers

(β-mannanase + α-galactosidase)
(2) Galactomanno-oligomers + H₂O → D-galactose +
manno-oligomers

(β-galactose dehydrogenase)
(3) D-Galactose + NAD⁺ → D-galactonic acid + NADH + H⁺

Kit size:                            100 assays
Method:                            Spectrophotometric at 340 nm
Total assay time:              ~ 80 min
Detection limit:                 1-100% of sample weight
Application examples: 
Seeds, milling fractions and food ingredients
Method recognition:      Novel method

Advantages

• Galactose dehydrogenase now included in the kit
  
• Very cost effective
  
• All reagents stable for > 2 years after preparation
  
• Only enzymatic kit available
  
• Simple format
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included

High purity recombinant beta-Xylosidase (B. pumilus) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.37 
CAZy Family: GH43

1,4-β-D-xylan xylohydrolase.

Recombinant from Bacillus pumilus (Bacillus mesentericus).
In 3.2 M ammonium sulphate.

Specific activity: ~20 U/mg (35^oC, pH 7.5).

The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

UV-method for the determination of Glucomannan in plant
products, foodstuffs and other materials

Principle:
(β-mannanase)
(1) Ac-Glucomannan + H₂O → Ac-glucomanno-oligomers

(pH 12.5)
(2) Ac-Glucomanno-oligomers + H₂O → glucomanno-oligomers
+ acetate

(β-glucosidase + β-mannosidase)
(3) Glucomanno-oligomers + H₂O → D-glucose + D-mannose

(hexokinase)
(4) D-glucose + D-mannose + ATP → G-6-P + M-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(phosphomannose isomerase) (phosphoglucose isomerase)
(6) M-6-P → F-6-P → G-6-P

Kit size:                            50 assays 
Method:                            Spectrophotometric at 340 nm
Total assay time:              120 min
Detection limit:                 1-100% of sample weight
Application examples: 
Jelly sweets, cosmetics, food gums and other materials
Method recognition:       Novel method

Advantages

• Very cost effective
  
• Only enzymatic kit available
  
• Simple format
  
• All reagents stable for > 2 years after preparation
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included

The Pectin Identification Assay Kit is suitable for the identification of pectin in food ingredients. This kit now employs a new pectate lyase from Aspergillus niger.

UV-method for the identification of Pectin in foodstuffs, feed
and fruit juice

Principle:
(pH 12.5)
(1) Pectin + H₂O → pectate + methanol

(pectate lyase)
(2) Pectate → 4,5-unsaturated oligogalacturonates

Kit size:                          500 assays
Method:                          Spectrophotometric at 235 nm
Reaction time:                 ~ 30 min
Detection limit:                N/A
Application examples: 
Food ingredients (e.g. citrus fruit and apple) and other materials
Method recognition:     
JECFA

Advantages

• Very cost effective
  
• All reagents stable for > 2 years after preparation
  
• Only enzymatic kit available
  
• Simple format
  
• Standard included

EC 3.2.1.37
CAZY Family: GH43
1,4-β-D-xylan xylohydrolase. Recombinant from S. ruminantium. In 3.2 M ammonium sulphate.
Specific activity: ~ 115 U/mg (40^oC, pH 5.3 on p-NP-β-D-xylanopyranoside).
Other activities: p-NP-α-L-arabinofuranoside ~ 7 %.

A simple method for the rapid and reliable measurement of acetic acid/acetate in foods, beverages and other materials. Content:53 assays per kit

Manual format UV-method for the determination of Acetic Acid in foodstuffs, beverages and other materials

Principle:
                           (acetyl-CoA synthetase)
(1) Acetic acid + ATP + CoA → acetyl-CoA + AMP + pyrophosphate

                                                (citrate synthase)
(2) Acetyl-CoA + oxaloacetate + H₂O → citrate + CoA

                (L-malate dehydrogenase)
(3) L-Malate + NAD⁺ ↔ oxaloacetate + NADH + H⁺

Kit size:                            53 assays
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 14 min
Detection limit:                 0.14 mg/L
Application examples: 
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, 
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products 
(and baking agents), ketchup, soy sauce, mayonnaise, dressings, 
paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), 
feed and other materials (e.g. biological cultures, samples, etc.) 
Method recognition:     
Methods based on this principle have been accepted by EN, ISO, 
ICUMSA, IFU and MEBAK

Advantages

• No wasted ACS solution (stable suspension supplied)
   
• PVP incorporated to prevent tannin inhibition
   
• All reagents stable for > 2 years after preparation

• Very competitive price (cost per test)
   
• Mega-Calc™software tool is available from our website for hassle-free raw data processing

High purity recombinant exo-1,4-beta-D-Xylosidase (S. ruminantium) for use in research, biochemical enzyme assays andin vitro diagnostic analysis.

EC 3.2.1.37 
CAZy Family: GH43

1,4-β-D-xylan xylohydrolase.

Recombinant from S. ruminantium. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 115 U/mg (40^oC, pH 5.3 on pNP-β-D-xylanopyranoside).

Data booklets for each pack size are located in the Technical Resources tab.

High purity Cellobiohydrolase I (T. longibrachiatum) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.176

From Trichoderma longibrachiatum. Electrophoretically homogeneous (MW 65,000). 
In 3.2M ammonium sulphate.

Specific activity: ~ 0.1 U/mg (40^oC, pH 4.5, CM-cellulose 4M as substrate). Active on p-nitrophenyl-β-lactoside.

Stable at 4^oC for > 4 years.