Typical Standard Curve for the DNA Damage (8-OHdG) ELISA kit (SKT-120). Competitive ELISA format, assay range: 0.94 – 60 ng/ml.
References
1. Mechanisms of DNA damage, repair and mutagenesis, Chatterjee N and Walker GC, Environ Mol Mutagen. 2017 Jun;58(5):235-263
2. DNA damage and the balance between survival and death in cancer biology, Roos WP et al, Nat Rev Cancer. 2016 16, 20–33
3. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling, Ray PD et al, Cell Signal. 2012 May;24(5):981-90
4. Mitochondrial reactive oxygen species as major effectors of antimicrobial immunity, Shekhova E, PLoS Pathog 2020 16(5)
5. Oxidative DNA damage: mechanisms, mutation, and disease, Cooke MS et al, FASEB J. 2003 Jul;17(10):1195-214
6. Oxidative Stress and Cancer, Klaunig JE, Curr Pharm Des. 2018;24(40):4771-4778
7. 8-hydroxy-2′ -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis, Valavanidis A et al, J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2009 Apr;27(2):120-39
Small cell lung cancer (SCLC) accounts for approximately 15% of all lung cancers. elta-like ligand 3 (DLL3) is an inhibitory Notch ligand that is highly expressed in SCLC and other neuroendocrine tumors but minimally expressed in normal tissues. It is therefore being explored as a potential therapeutic target in SCLC.
细胞定位
Membrane
UniProt
Q9NYJ7
实验结果图
WB result of DLL3 Rabbit mAb Primary antibody: DLL3 Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: TT whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 65 kDa Observed MW: 60 kDa Exposure time: 180s
IHC shows positive staining in paraffin-embedded human medullary thyroid carcinoma. Anti-DLL3 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human small cell lung cancer. Anti-DLL3 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-DLL3 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-DLL3 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-DLL3 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows membrane staining in TT cells. Anti-DLL3 antibody was used at 1/1000 dilution and incubated overnight at 4°C. Secondary antibody: #JP20025 at 1/1000 dilution.The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
Negative control: ICC shows negative staining in HeLa cells. Anti-DLL3 antibody was used at 1/1000 dilution and incubated overnight at 4°C. Secondary antibody: #JP20025 at 1/1000 dilution.The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
The T1/Leu1/CD5 molecule, a human T-cell surface glycoprotein of relative molecular mass (Mr) 67,000, has been implicated in the proliferative response of activated T cells and in T-cell helper function. Ly1 is the murine homolog. (In the mouse, lymphocyte surface antigens that are markers for function have been identified. Allelic forms are known and chromosomal assignment of the loci has been achieved. Ly1 is on mouse chromosome 19. Ly2 and Ly3 are on mouse chromosome 6. Ly5 is on mouse chromosome 1. Ly6 is on mouse chromosome 15.
Clone
hCD5.
Immunogen
Human PBL.
Ig Subclass
Mouse IgG1.
Purification Method
Ion exchange column.
Protein concentration
1mg/ml in PBS (after reconstitution).
Storage Procedures
Lyophilized: store at 4oC. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.
Reconstitution
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Antibody Properties
Staining antibody. For staining, use 10µl/1,000,000 cells.
Available Conjugates
This antibody is only available in purified form.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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