unseparated blood3, bone marrow4, or fetal liver cells
sorted cell populations1,4
or ES and iPS cell-derived hematopoietic precursors2

StemMACS HSC-CFU Media are standardized semi-solid media based on methylcellulose in IMDM, supplemented with FBS, BSA, and different growth factors. StemMACS HSC-CFU media are designed to maximize growth and differentiation of progenitor cells and allow the clonal progeny of a single cell to grow in a distinct cluster or colony. They are produced under tightly controlled manufacturing conditions and use highly qualified raw materials to provide a consistent and optimally performing colony assay.

StemMACS HSC-CFU media are available in different formats.

 Miltenyi Biotec Inc.
2303 Lindbergh Street, Auburn, CA 95602, USA
Phone 800 FOR MACS, +1 530 888 8871, +1 530 888 8925
macs@miltenyibiotec.com
page 1/3
Miltenyi Biotec GmbH
Friedrich-Ebert-Straße 68, 51429 Bergisch Gladbach, Germany
Phone +49 2204 8306-0, +49 2204 85197
macs@miltenyibiotec.de
www.miltenyibiotec.com 140-001-101.06
Contents
1. Description
1.1 Background information
1.2 Product specification
1.3 Reagent and instrument requirements
2. Protocol
2.1 Preparation of StemMACS HSC-CFU Media
2.2 Preparation of cell samples
2.3 Set-up of CFU assay
2.4 Reading the plate
3. Appendix: Tips & hints
4. References
1. Description
Components

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