X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Medium
货号：BE04-418F
厂家：Lonza公司
品牌：BioWhittaker® X-VIVO™
规格：1L
产品应用：肿瘤浸润淋巴细胞，CD3+等细胞的培养
保存温度：2～8℃
会员优惠价格： 请来电
货期：3-4周
BioWhittaker® X-VIVO™ BE04-418F X-VIVO™ 15 含酚红化学成分确定的无血清造血干细胞培养基
英文名称： X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Medium
产品应用： 肿瘤浸润淋巴细胞，CD3+等细胞的培养
中国俗称： 无血清培养基 中国简称： 无血清培养基
注册品牌： BioWhittaker® X-VIVO 生产厂家： 美国Lonza公司
订购货号： BE04-418F
规格型号： 500ml/瓶
保存温度： 2～8 ℃
产品种类： 淋巴细胞培养基及相关产品
到货时间： 3～4周
产品编码： YS000646
产品中文名称：X-VIVO™ 15化学成分确定的无血清造血细胞培养基
X-VIVO™ Chemically Defined, Serum-free Hematopoietic Cell Medium研究背景
Lonza’s R&D efforts and collaboration with many clinical trials have provided Lonza with the tools and expertise to support the developments in adoptive immunotherapy, cancer therapy, genetic therapy and other cellular therapies. Lonza’s philosophy for the development of serum-free formulations for use in cellular therapy is to provide a nutritionally complete and balanced environment for the cells. We have not included any exogenous growth factors, artificial stimulators of cellular proliferation or undefined supplements.
The products are devoid of any protein-kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes. The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin and pasteurized human transferrin. All X-VIVO™ Medium products are manufactured under current GMPs and are listed with the FDA in a product Master File. Permission to cross-reference the Master File may be obtained by contacting the Product Manager.
X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Medium 简介
X-VIVO™ 15 Medium is similar in composition to X-VIVO™ 10 Medium and has been optimized for the proliferation of Tumor Infiltrating Lymphocytes （TIL） under serum-free conditions. The formulation supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors. X-VIVO™ 15 Medium has also been used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes and natural killer （NK） cells. In addition, X-VIVO™ 15 Medium provides a serum-free environment for the expansion of HUT-78 and related human lymphocytic cell lines.
X-VIVO™ 15化学成分确定的无血清造血细胞培养基特点
» 不含血清
» 化学成份明确
» 成份包含L-谷氨酰胺，庆大霉素和酚红
» zui适于无血清环境中肿瘤浸润淋巴细胞（TIL）增殖
X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Medium 使用注意事项
1.X-VIVO™ 15 is similar in composition to X-VIVO™ 10 and has been optimized for the proliferation of Tumor Infiltrating Lymphocytes （TIL） under serum-free conditions.
2.Please see Supplemental Information for additional hematopoietic cell applicat.
在仓库备有现货，欢迎新老客户：
Lonza UltraCULTURE™无血清培养基,500ml,不含L-谷氨酰胺
英文名称： UltraCULTURE™ Serum-free Medium
产品应用： 哺乳动物细胞培养，病毒颗粒生成等
中国俗称： 无血清培养基 中国简称： 无血清培养基
注册品牌： Lonza 生产厂家： 美国Lonza公司
订购货号： 12-725F
规格型号： 500ml/瓶
保存温度： 2～8℃
产品种类： 无血清培养基
到货时间： 上海仓库有现货，1～5天到货
产品编码： YS000235
产品英文名称：LONZA 12-725F UltraCULTURE™ Serum-free Medium
产品中文名称：UltraCULTURE™无血清培养基
LONZA 12-725F UltraCULTURE™ Serum-free Medium产品介绍：
LONZA 12-725F UltraCULTURE™无血清培养基是一个完全、通用的培养基，设计用于各种粘附型和非粘附型哺乳动物细胞的培养。UltraCULTURE™无血清培养基可用于杂种细胞生成过程中促进细胞融合，促进单核细胞、巨噬细胞、上皮细胞和成纤纤细胞系的增殖生长，并且有助于疫苗生产中病毒颗粒的产生。培养基中加入了重组人胰岛素，牛转铁蛋白和牛血清蛋白纯化混合物。上海思创生物公司在上海仓库长期储备现货，以优惠的价格向广大生命科学研究员和细胞培养专业技术人员提供此款通用细胞治疗级的LONZA 12-725F UltraCULTURE™ Serum-free Medium, general purpose, without L-glutamine SFM无血清培养基
UltraCULTURE™培养基总蛋白浓度接近3mg/ml。UltraCULTURE™培养基可以作为防冷冻培养基(Cat.No.12-132A)补充物便于冻存细胞在无血清环境中培养。LONZA 12-725F UltraCULTURE™无血清培养基不含L-glutamine(L-谷氨酸盐)；用之前添加5ml 200mM/L L-谷氨酸盐溶液(Cat.Nos.17-605 or 17-905)。
LONZA UltraCULTURE™ Serum-free Medium产品性能及应用：
» 用于粘附和非粘附性哺乳动物细胞培养
» 疫苗产品中病毒颗粒的生成
» UltraCULTURE™培养基中可能会出现混浊，但不影响产品性能
UltraCULTURE™ Serum-free Medium英文简述：
LONZA 12-725F UltraCULTURE™ Serum-free Medium is a complete, allpurpose medium designed for the cultivation of a wide variety of adherent and non-adherent mammalian cell types. UltraCULTURE™ Medium can be used to support fusion of cells during hybridoma formation, growth of monocyte, macrophage, epithelial, and fibroblastic cell lines, and generation of virus particles for vaccine production. The medium is supplemented with recombinant human insulin, bovine transferrin, and a purified mixture of bovine serum proteins. The total protein concentration of UltraCULTURE™ Medium is approximay 3 mg/ml. UltraCULTURE™ Medium can be supplemented with Cryoprotective Medium (Cat. No. 12-132A) to cryopreserve cells in a serum-free environment. UltraCULTURE™ Medium does not contain L-glutamine; please add 5 ml of 200 mM L-glutamine solution (Cat. Nos. 17-605 or 17-905) prior to use.
The formulation for UltraCULTURE™ Medium has been submitted to the FDA as a Master File. Permission to crossreference the Master File may be obtained by contacting the Regulatory Affairs Department.
Applications
– Cultivation of adherent and non-adherent mammalian cells
– Generation of viral particles for vaccine production
Performance and Quality Tests
– Turbidity may develop in UltraCULTURE™ Medium; experiments have determined that the turbidity will not alter the performance of the product
