80-CPTRT-E01-Alpco 80-CPTRT-E01 大鼠C-肽ELISA检测试剂盒,10µl,96孔-ELISA检测试剂盒

产品英文名称:Rat C-peptide ELISA Kit
产品中文名称:大鼠C-肽ELISA检测试剂盒
货号:80-CPTRT-E01
厂家:美国Alpco公司
品牌:Alpco
规格:96T/盒
产品应用:小鼠C-肽ELISA检测
保存温度: 2-8℃
货期:2~5天
详情请。

产品英文名称:Rat C-peptide ELISA Kit
产品中文名称:大鼠C-肽ELISA检测试剂盒

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§ 检测原理:ALPCO 大鼠C-肽 ELISA设计用于检测大鼠血清中C-肽含量。ALPCO大鼠C-肽ELISA是一个夹层式免疫分析试剂盒。96孔微型板涂有一层C-肽特异性单克隆抗体。检测分析使用有机合成C-肽蛋白分子量标定。将标准品,对照物和样品加到孔中和配体物混合。后放微孔板摇床上温育,700-900rpm。*次温育结束,用清洗缓冲液清洗小孔并吸干。加TMB作用底物,第二次温育,700-900rpm。第二次温育完成,加终止液,调节分光光度计到450nm测定光密度(O.D.)。由于光密度与样品中C-肽含量成比例,所以可以根据O.D.值计算出C-肽含量。

§ 检测过程:提前从冰箱中取出试剂盒,平衡至室温→各移取10µl标准品,对照品和样品到每个小孔中→移取100µl结合物到每孔中(配制方法参照说明书)→密封微孔板,放在微孔板摇床上室温温育2小时,700-900rpm→清空小孔,350µl清洗缓冲液清洗三次(每次清洗具体方法不一样,参照说明书)→每孔添加100µlTMB作用底物→密封微孔板,放在微孔板摇床上室温温育10分钟,700-900rpm→每孔添加100µl反应终止液,轻摇混合,清除气泡再准备下一步操作→放在微孔板读数器,调节分光光度计到450nm读数(30分钟内)

ALPCO RAT C-peptide ELISA kit英文简述:
The ALPCO Rat C-peptide ELISA kit quantifies the C-peptide protein products of the rat I and rat II proinsulin genes. Utilizing a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips, a single Rat C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.

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