Anti-Cdk9 antibody 抗细胞周期依赖性激酶抗体
货号：ab124261
厂家：Abcam公司
规格：40ul/100ul
保存温度：-20℃ 和 2～8℃
货期：现货2～5天，期货2-3周
Anti-Cdk9 antibody 抗细胞周期依赖性激酶抗体
- Product nameAnti-Cdk9 antibodySee all Cdk9 primary antibodies …
- Description
Rabbit polyclonal to Cdk9
- Tested applicationsIP more details
- Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan - Immunogen
Synthetic peptide, corresponding to to a region within amino acids 1-50 of Human Cdk9 (NP_001252.1).
- Positive control
- HeLa whole cell lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: pH 7 to 8 -
Concentration 100 µg at 1 mg/ml
- PurityImmunogen affinity purified
- Purification notesab124261 was affinity purified using an epitope specific to Cdk9 immobilized on solid support .
- Clonality Polyclonal
- IsotypeIgG
-
Applications
Our Abpromise guarantee covers the use of ab124261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Notes IP IP: Use at 2-10 µg/mg of lysate. - Application notesIs unsuitable for or WB.
-
Target
- FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Cellular localizationNucleus.
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Alternative names
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
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Anti-Cdk9 antibody images
Immunoprecipitation – Anti-Cdk9 antibody (ab124261)
Anti-Cdk9 antibody which recognizes a downstream epitope of Cdk9 was used at 1 µg/ml detecting Cdk9 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with ab124261 at 6 µg/mg of lysate.
Lane 2: IP with an antibody which recognizes an downstream epitope of Cdk9.
Lane 3: Control IgG.In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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