ab52600-Anti-hnRNP K antibody EP943Y (ab52600)抗核糖核蛋白K抗体-抗体/抗原

Anti-hnRNP K antibody [EP943Y] (ab52600)抗核糖核蛋白K抗体
货号:ab52600
厂家:Abcam公司
规格:40ul/100ul
保存温度:-20℃ 和 2~8℃
货期:现货2~5天,期货2-3周

咨询:  (重庆) (贵阳)

Anti-hnRNP K antibody [EP943Y] (ab52600)抗核糖核蛋白K抗体产品信息:

  • Product nameAnti-hnRNP K antibody [EP943Y]
  • Description

    Rabbit monoclonal [EP943Y] to hnRNP K

  • SpecificityThis antibody detects both phosphorylated and unphosphorylated hnRNP K.
  • Tested applicationsWB, IP, IHC-P, ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human hnRNP.

  • Positive control
    • Jurkat cell lysate; HeLa cells; Human breast carcinoma tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • Clonality Monoclonal
  • Clone numberEP943Y
  • IsotypeIgG
  • Research Areas
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Applications

Our Abpromise guarantee covers the use of ab52600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/100000. Detects a band of approximay 60 kDa (predicted molecular weight: 51 kDa).
IP IP: 1/50.
IHC-P IHC-P: Use at an assay dependent dilution.
ICC/IF ICC/IF: 1/100 – 1/250.

Target

  • FunctionOne of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • Sequence similaritiesContains 3 KH domains.
  • Post-translational
    modificationsArg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationCytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Database links

    • Entrez Gene: 3190 Human
    • Entrez Gene: 15387 Mouse
    • Entrez Gene: 117282 Rat
    • Omim: 600712 Human
    • SwissProt: P61978 Human
    • SwissProt: P61979 Mouse
    • SwissProt: P61980 Rat
    • Unigene: 522257 Human

    see all

  • Alternative names

    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody

    see all

Anti-hnRNP K antibody [EP943Y] images

  • ab52600-Anti-hnRNP K antibody EP943Y (ab52600)抗核糖核蛋白K抗体-抗体/抗原

    Western blot – hnRNP K antibody [EP943Y] (ab52600)

    Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/100000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 51 kDa
    Observed band size : ~60 kDa (why is the actual band size different from the predicted?)

  • ab52600-Anti-hnRNP K antibody EP943Y (ab52600)抗核糖核蛋白K抗体-抗体/抗原

    Immunohistochemistry (Paraffin-embedded sections) – hnRNP K antibody [EP943Y] (ab52600)

    Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using ab52600 at a dilution of 1/100-1/250.

  • ab52600-Anti-hnRNP K antibody EP943Y (ab52600)抗核糖核蛋白K抗体-抗体/抗原

    Immunocytochemistry/ Immunofluorescence – hnRNP K antibody [EP943Y] (ab52600)

    Fluorescent immunostaining of HeLa cells using ab52600 at a dilution of 1/100-1/250.

公司介绍:

    Abcam位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。主要创立人Jonathan Milner和实验室同事构思成立一间了解科研人员需要的网上抗体公司,理念是Abcam销售质量高的抗体,并提供zui全面、诚实、时新的说明书,快速的送货服务,及高效率的顾客和服务。我们的在线目录(www.hystemcell.cn) 已有差不多100,000种抗体和试剂,并不断添加,供应予全球百多个国家。Abcam200511月在伦敦证券交易所上市,在美国马萨诸塞州、日本东京及香港均设有分公司。

    Abcam 一直致力加强产品线,为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的MitoSciences公司,加强了免疫分析方面的产品供应;同年也并购了英国的Ascent Scientific 公司,开展了生化试剂的供应。在2012年,Abcam并购了美国的Epitomics 公司,成为一家有领导地位的RabMAbs® 供应商。

    Abcam 的 目标是给世界上zui好的抗体建立zui大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴。我们为所有产品提供来使客户获得预期的结 果。为提供高质量的抗体来指向尽可能多的靶蛋白,我们尽所能在尽可能多的应用和物种中检测每种抗体。我们相信诚信才是上策,在我们的上会发布有关我们 每一种产品的尽可能多的信息。

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