Anti-HEPACAM [EPR11217] antibody (ab172635)抗肝细胞黏附分子抗体
货号：ab172635
厂家：Abcam公司
规格：40ul/100ul
保存温度：-20℃ 和 2～8℃
货期：现货2～5天，期货2-3周
咨询： （重庆） （贵阳）
Anti-HEPACAM [EPR11217] antibody (ab172635)抗肝细胞黏附分子抗体产品信息：
- Product nameAnti-HEPACAM [EPR11217] antibody
- Description
Rabbit monoclonal [EPR11217] to HEPACAM
- Tested applicationsWB, ICC/IF
- Species reactivityReacts with: Human
Does not react with
Mouse, Rat
- Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HEPACAM aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14CZ8 - Positive control
- Human fetal kidney, fetal liver, fetal brain and stomach lysates; HepG2 cells.
- General notes
Produced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.05% BSA - PurityTissue culture supernatant
- ClonalityMonoclonal
- Clone numberEPR11217
- IsotypeIgG
- Research Areas
- Signal Transduction
- Cytoskeleton / ECM
- Cell Adhesion
- Cell Adhesion Molecules
- Liver
- Cancer
- Invasion/microenvironment
- ECM
- Cell adhesion
- Other
Applications
Our Abpromise guarantee covers the use of ab172635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000 – 1/10000. Detects a band of approximay 72 kDa (predicted molecular weight: 46 kDa). |
| ICC/IF | ICC/IF: 1/250 – 1/500. |
- Application notesIs unsuitable for ,IHC-P or IP.
Target
- FunctionInvolved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.
- Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. - DomainThe cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility.
- Post-translational
modificationsN-glycosylated. - Cellular localizationCytoplasm. Membrane. In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.
-
Database links
- Entrez Gene: 220296 Human
- Omim: 611642 Human
- SwissProt: Q14CZ8 Human
- Unigene: 159863 Human
Anti-HEPACAM [EPR11217] antibody images
Immunocytochemistry/ Immunofluorescence – Anti-HEPACAM [EPR11217] antibody (ab172635)
-
Immunofluorescent analysis of HepG2 cells labeling HEPACAM with ab172635 at 1/250 dilution. DAPI nuclear staining (blue).
公司介绍：
Abcam位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。主要创立人Jonathan Milner和实验室同事构思成立一间了解科研人员需要的网上抗体公司，理念是Abcam销售质量高的抗体，并提供zui全面、诚实、时新的说明书，快速的送货服务，及高效率的顾客和服务。我们的在线目录(www.abcam.cn) 已有差不多100,000种抗体和试剂，并不断添加，供应予全球百多个国家。Abcam于2005年11月在伦敦证券交易所上市，在美国马萨诸塞州、日本东京及香港均设有分公司。
Abcam 一直致力加强产品线，为使研究员更容易找到蛋白质研究试剂产品，在2011并购了美国的MitoSciences公司，加强了免疫分析方面的产品供应；同年也并购了英国的Ascent Scientific 公司，开展了生化试剂的供应。在2012年，Abcam并购了美国的Epitomics 公司，成为一家有领导地位的RabMAbs® 供应商。
Abcam 的 目标是给世界上zui好的抗体建立zui大的在线目录，为各地科学家提供尖端产品，成为各国科学界的重要伙伴。我们为所有产品提供来使客户获得预期的结 果。为提供高质量的抗体来指向尽可能多的靶蛋白，我们尽所能在尽可能多的应用和物种中检测每种抗体。我们相信诚信才是上策，在我们的上会发布有关我们 每一种产品的尽可能多的信息。
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