BioLife Solutions CryoStor CS10 210102细胞DMSO冻存液-细胞冻存液

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BioLife Solutions CryoStor CS10 210102细胞DMSO冻存液:
BioLife Solutions CryoStor CS10 细胞冻存液预混10% DMSO,适用于高效冻存多种类型细胞。

 

品牌:BioLife Solutions

货号:PART No. 210102

容积:VOL. 100mL

型号:CryoStor CS10

 

BioLife Solutions CryoStor CS10细胞DMSO冻存液210102订购信息

 

品牌

货号

产品描述

规格

BioLife Solutions

210102

CryoStor CS10 Freeze Media 细胞DMSO冻存液

100mL /瓶

 

BioLife Solutions CryoStor CS10 210102细胞DMSO冻存液-细胞冻存液

 

英文名BioLife Solutions CryoStor CS10 Freeze Media

中文名:BioLife Solutions CryoStor CS10 细胞DMSO冻存液

BioLife Solutions CryoStor CS10细胞DMSO冻存液210102 代理销售 再生医学旗舰公司:红荣微再(上海)生物工程技术有限公司  :152 1681 4001。红荣微再-客服: 316 808 6348  经销商专员

 

BioLIfe Solutions CryoStor CS10细胞DMSO冻存液产品系列

CryoStor®, a series of cell-specific, optimized freeze media, is designed to prepare and preserve cells in ultra low temperature environments (-70ºC to -196ºC); CryoStor®, pre-formulated with DMSO, provides a safe, protective environment for cells and tissues during the freezing, storage, and thawing process. Through modulating the molecular-biological response to the cryopreservation process, CryoStor® provides for enhanced cell viability and functionality while eliminating the need for serum, proteins or high levels of cytotoxic agents.

 

BioLife Solutions CryoStor CS10 210102细胞DMSO冻存液-细胞冻存液 

各种规格的BioLIfe Solutions CryoStor 细胞DMSO冻存液

 

BioLife Solutions CryoStor®是BioLife公司特别优化的细胞冻存液,在极低温度 (-70ºC to -196ºC)下准备和保存细胞。预混DMSO,为细胞和组织的冷冻、储存和解冻过程提供安全的保护环境。CryoStor®通过调控冻存过程的分子生物学反应,无血清、蛋白或具有高细胞毒性的试剂,就能增强细胞活力和功能。

 

BioLife Solutions CryoStor CS10细胞冻存液特点

用于细胞和组织的高级生物保存液:

预混DMSO

无血清、无蛋白

USP级高质量成分、cGMP级生产、FDA master文件

灭菌、内毒素和细胞功能检测

 

品牌

货号

产品描述

规格

BioLife Solutions

210102

CryoStor CS10 Freeze Media 细胞DMSO冻存液

100mL /瓶

BioLife Solutions

210210

CryoStor CS10 Freeze Media 细胞DMSO冻存液

1000mL/袋

BioLife Solutions

210202

CryoStor CS10 Freeze Media 细胞DMSO冻存液

100mL/袋

BioLife Solutions

210374

CryoStor CS10 Freeze Media 细胞DMSO冻存液

16mL /瓶

BioLife Solutions

210373

CryoStor CS10 Freeze Media 细胞DMSO冻存液

10mL /瓶

 

BioLife Solutions CryoStor CS10 细胞DMSO冻存液cGMP级生产

BioLife Solutions CryoStor CS10 210102细胞DMSO冻存液-细胞冻存液 

BioLife Solutions CryoStor CS10 Freeze Media 使用与冻存方法

CryoStor® Usage and Cryopreservation Protocol

细胞准备

1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)

2) Centrifuge cells to obtain cell pellet

3) Remove supernatant – Note: Remove as much culture media as possible, to reduce dilution of CryoStor® solution.

加CryoStor冻存液

4) ISOLATION: Add cold (2-8°C) CryoStor®

a. Cell concentrations: 0.5-10 x 106

 cells/ml for routine cell culture protocols (higher [cell] possible).

b. DMSO is pre-mixed in CryoStor® – no additives are necessary.

5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes

6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)

a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.

b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.

c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.

d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.

BioLife Solutions 储存

7) STORAGE: Place samples into storage

a. Store samples at liquid nitrogen temperatures (below -130°C).

b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).

细胞复苏解冻

8) THAWING: Thaw samples quickly in a 37°C water bath

a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.

b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.

Passive thaw is not recommended.

9) Dilute cell/CryoStor® mixture immediay with culture media

a. Dilution procedure can be preformed in a single step.

b. The dilution media should be between 20°C and 37°C.

c. A dilution ratio of 1:10 (sample to media) or greater is recommended.

10) Plate cells in appropriate configuration

11) Place cells into culture conditions or utilize immediay

12) Viability assessment 24-hours post-thaw*

BioLife Solutions Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.

*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.

Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue®) are recommended for more accurate viability assessment.

Visual inspection of adherent cells and cells “floating” in the media is also recommended.

 

BioLife Solutions CryoStor CS10细胞DMSO冻存液210102 代理销售

  

以“专注干细胞研究,提升干细胞品质为发展理念,专业从事干细胞基础研究、干细胞再生医学临床应用研究及干细胞技术服务。