Cellartis Y00275-Human iPS Cell Lines人iPS诱导多能干细胞-iPS诱导多能干细胞株

品牌 其他品牌 货号 Y00185/Y00275/Y00285/Y00315/Y00325/Y0030
规格 套装 供货周期 一个月
主要用途 细胞治疗、药物监测和疾病模型等

Human iPS Cell Lines人iPS诱导多能干细胞产品包括人iPS诱导多能干细胞和用于增殖培养的Cellartis DEF-CS Culture System培养体系。细胞系的纯度和干细胞特征经过广泛分析。细胞在分化时可以分化形成所有三个胚层的衍生物。

   

集团旗下授权代理TTAKARA Cellartis干细胞产品

图片为展示效果。实际产品为细胞株+培养基套装

 

人iPS诱导多能干细胞

(Human Induced Pluripotent Stem Cells)

 

产品英文名称:Cellartis® Human iPS Cell Lines

产品中文名称:Cellartis® 人iPS诱导多能干细胞

 

Cellartis人iPS细胞(Human iPS Cell Line)产品是达到了工业客户使用标准生产的干细胞产品。该产品包括细胞和用于增殖培养的Cellartis DEF-CS Culture System培养体系。iPS细胞已经在制品化时完成了对DEF-CS培养体系的适应。Cellartis DEF-CS Culture System培养体系是组分明确而且不依赖饲养层的便捷的干细胞培养体系。Cellartis DEF-CS Culture System适合高效率地增殖培养干细胞,并且保持干细胞在增殖过程中几乎无分化地生长,这使得不需要在大规模增殖培养过程中进行细胞筛选。因此,Cellartis人iPS细胞(Human iPS Cell Line)产品是一套完整的产品体系,对于科研级别和工业规模的需求都适合。

 

Cellartis人iPS诱导多能干细胞特点

细胞系通过使用缺陷型多顺反子逆转录病毒(defective polycistronic retrovirus)技术重编程人皮肤成纤维细胞以转入Oct-4,SOX2,KLF4和c-Myc

细胞系的纯度和干细胞特征经过广泛分析,包括解冻后的恢复,支原体和细菌检测,干细胞特异性标记物的表达(Oct-4,NANOG,SSEA-3,SSEA-4,TRA-1 -60,TRA-1-81),不表达分化标记(β-3微管蛋白,FOXA2,ASMA)和预期核型

用Cellartis DEF-CS培养系统优化培养,试剂盒包括Cellartis DEF-CS 100培养系统

适用于所有主要应用; 细胞在分化时可以分化形成所有三个胚层的衍生物

Cell lines were created by reprogramming human skin fibroblasts using defective polycistronic retrovirus technology to deliver Oct-4, SOX2, KLF4, and c-Myc

Cell lines are extensively analyzed for purity and stem-cell characteristics, including recovery after thawing, absence of mycoplasma and bacteria, expression of stem cell-specific markers (Oct-4, NANOG, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), absence of differentiation markers (Beta-3 tubulin, FOXA2, ASMA), and expected Karyotype

Optimized for culture with the Cellartis DEF-CS Culture System

Suitable for all major applications; cells can form derivatives of all three germ layers when differentiated

Kits include the Cellartis DEF-CS 100 Culture System

 

Cellartis 人iPS诱导多能干细胞参数

不同货号对应供体年龄性别冷冻代数和已确认分化能力

 

Cellartis Y00275-Human iPS Cell Lines人iPS诱导多能干细胞-iPS诱导多能干细胞株 

 

iPS诱导多能干细胞的应用

细胞治疗、药物监测和疾病模型

 

Cellartis 人iPS诱导多能干细胞组份

不同供体的iPS细胞系和DEF-CS 100完全培养基

完全培养基包含基础培养基、添加剂和包被剂。

保存:细胞 ≤ –150°C,添加剂 –20°C,基础培养基和包被剂4 °C

 

A. Cellartis Human iPS Cell Lines

Cellartis human iPS cell line P11025 (Cat. No. Y00180, not sold separay; sold as a part of Cat.No. Y00185)

Cellartis human iPS cell line P11032 (Cat. No. Y00220, not sold separay; sold as a part of Cat.No. Y00225)

Cellartis human iPS cell line 4 (ChiPSC4) (Cat. No. Y00260, not sold separay; sold as a part of Cat. No. Y00265)

Cellartis human iPS cell line 7 (ChiPSC7) (Cat. No. Y00270, not sold separay; sold as a part of Cat. No. Y00275)

Cellartis human iPS cell line 12 (ChiPSC12) (Cat. No. Y00280, not sold separay; sold as a part of Cat. No. Y00285)

Cellartis human iPS cell line 18 (ChiPSC18) (Cat. No. Y00300, not sold separay; sold as a part of Cat. No. Y00305)

Cellartis human iPS cell line 21 (ChiPSC21) (Cat. No. Y00310, not sold separay; sold as a part of Cat. No. Y00315)

Cellartis human iPS cell line 22 (ChiPSC22) (Cat. No. Y00320, not sold separay; sold as a part of Cat. No. Y00325)

B. Cellartis DEF-CS 100 Culture System

· Cellartis DEF-CS 100 Culture System (Cat. No. Y30020, not sold separay; sold as a part of Cat.Nos. Y00185, Y00225, Y00265, Y00275, Y00285, Y00305, Y00305, and Y00315. A larger 500ml version of this product is sold as Cat. No. Y00310.)

o DEF-CS Basal Medium (100 ml)

o DEF-CS COAT-1 (for 100 ml) (800 µl)

o DEF-CS GF-1 (for 100 ml) (300 µl)

o DEF-CS GF-2 (for 100 ml) (100 µl)

o DEF-CS GF-3 (for 100 ml) (40 µl)

 

另行准备材料:PBS+TrypLE 选择酶+TC处理ps表面培养器皿

The following materials are required but not supplied:

· PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+)

· PBS Dulbecco's without Ca2+ or Mg2+ (D-PBS –/–)

· TrypLE Select Enzyme (1X), no phenol red

· Cell culture vessels, Tissue culture treated polystyrene surface

· General cell culture equipment used in cell culture laboratory

 

参考文献:

1. de Peppo GM, et al. Osteogenic potential of human mesenchymal stem cells and human embryonic stem cell-derived mesodermal progenitors: a tissue engineering perspective. Tissue Eng Part A.2010 Nov;16(11):3413-3426.

2. Both SK, et al. Differential bone-forming capacity of osteogenic cells from either embryonic stem cells or bone marrow-derived mesenchymal stem cells. J Tissue Eng Regen Med.2011 Mar;5(3):180-190.

3. de Peppo GM, et al. Free-form-fabricated commercially pure Ti and Ti6A14V Porous Scaffolds support the growth of human embryonic stem cell-derived mesodermal progenitors. ScientificWorldJournal. 2012;2012:646417.Epub 2012 Jan 4.

4. Ou Li, et al. Human embryonic stem cell-derived mesenchymal stroma cells(hES-MSCs) engraft in vivo and support hematopoiesis without suppression immune function: implications for off –the shelf ES-MSC therapies. PLOS ONE.2013; 8(1):e55319.

 

Cellartis 人iPS诱导多能干细胞订购信息

 

不同货号的区别:iPS细胞供体不同

 

品牌

Code No.

包装量

价格

Cellartis

Y00185

1 Kit

22718 元

Cellartis

Y00275

1 Kit

22718 元

Cellartis

Y00285

1 kit

22718 元

Cellartis

Y00315

1 Kit

22718 元

Cellartis

Y00325

1 kit

22718 元

Cellartis

Y00305

1 Kit

22718 元

 

 ​集团旗下授权代理TTAKARA Cellartis干细胞产品,Cellartis的多能细胞培养系统包括iPS诱导多能干细胞株、iPS诱导多能干细胞和ES胚胎干细胞培养基,iPS基因编辑用单细胞克隆培养基和多能细胞检测用单克隆抗体。

以“专注干细胞研究,提升干细胞品质”为发展理念,专业从事干细胞基础研究、干细胞再生医学临床应用研究及干细胞技术服务,自主研发产品热卖中:人间充质干细胞无血清培养基