12658-019-Sf-900™ III SFM (1X), liquid-Gibco血清

【简单介绍】

低水解液、无血清、无蛋白,对斜纹夜蛾的生长和维持动物的起源–优化免费的昆虫细胞培养基(Sf9和Sf21)细胞和重组基因的表达,利用杆状病毒和昆虫表达系统稳定

【详细说明】

描述

Sf-900™ III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for recombinant gene expression using the baculovirus and stable insect expression systems. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Features of Gibco® Sf-900™ III SFM:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, animal origin–free, ready-to-use formulation
• Improved lot-to-lot consistency

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ III SFM achieve maximum cell densities of 10 to 14 × 106 cells/mL, a significant improvement over Sf-900™ II SFM and other commercially available serum-free media (see figure).

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ III SFM is an improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins.

Serum-free, protein-free, animal origin–free, ready-to-use formulation
Gibco® Sf-900™ III SFM is a serum-free, protein-free, animal origin–free medium that allows for much easier purification of your protein of interest. Sf-900™ III SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900™ III SFM (see product manual for details).

Improved lot-to-lot consistency
Gibco® Sf-900™ III SFM contains reduced hydrolysate concentrations, improving lot-to-lot consistency over Sf-900™ II SFM 

规格

Form: Liquid
Species: Spodoptera frugiperda, S. frugiperda
Cell Line: Sf21, Sf9
Glutamine: L-Glutamine
Serum Level: Serum-Free
Product Size: 500 mL
Classification: Animal Origin-Free, Protein-Free, Serum-Free
Shipping Condition: Room Temperature

内容及储存

Storage conditions: 2–8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture