E885 DAB SUBSTRATE SYSTEM Grade:BIOTECHNOLOGY GRADE CAS Number: N/ASynonym(s): 3,3'-Diaminobenzidine Molecular Weight: N/AMolecular Formula: N/A Storage Condition: COLD CodeSizePriceQuantity E885-200ML Complete substrate system includes 20 DAB substrate tablets, peroxidase solution and a mixing/dispensing dropper bottle for fast and easy preparation. Each tablet will yield 10 ml of substrate solution, enough to effectively detect 50 cm2 of membrane or about 20 slides. Commonly used for detection of horseradish peroxidase in membrane-bound and In Situ applications. Insoluble end product is resistant to alcohol and fading, making it ideal for immunohistochemistry. Reaction Condition: 6 mg in 10 ml of 50 mM Tris, 0.1 M NaCl, pH 7.6 and 0.01% H2O2. The presence of Nickel or Cobalt increases sensitivity.