21555-PIERCE，DISUCCINIMIDYL SUBERATE （DSS）-琼脂糖

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【简单介绍】

货号：21555，名称：DISUCCINIMIDYL SUBERATE (DSS)，品牌：PIERCE，规格：1 GM

【详细说明】

订货：，，

 

DISUCCINIMIDYL SUBERATE （DSS）介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

DISUCCINIMIDYL SUBERATE （DSS）现货供应

 

PIERCE

21555

DISUCCINIMIDYL SUBERATE （DSS） ，1 GM

1 GM

PIERCE

21578

DTSSP, 3,3′-Dithiobis （Sulfosuccinimidylpropionate）

50 MG

PIERCE

21580

BS3, Bis（Sulfosuccinimidyl） Suberate

50 MG

 

DISUCCINIMIDYL SUBERATE （DSS）品牌介绍

 

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括：

1）化学发光体和比色底物

 

2）分析试剂

 

3）荧光标记探针

 

4）酶联免疫分析试剂盒等

 

DISUCCINIMIDYL SUBERATE （DSS）仅用于科研

 

Thermo Scientific Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide （NHS） ester at each end of an 8-carbon spacer arm.

 

NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.

 

DSS is first dissolved in an organic solvent such as DMF or DMSO, then added to the aqueous crosslinking reaction. BS3, the water soluble analog of DSS is also available for applications that require a hydrophilic crosslinker （e.g. to effect cell-surface crosslinking）. DSS and BS3 have essentially identical crosslinking activity toward primary amines.

DSS Highlights

  • Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • DSS crosslinker is membrane permeable, so intracellular crosslinking can be done
  • High purity, crystalline DSS can be used to create high-purity conjugates

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