21578-PIERCE，DTSSP, 3,3′-Dithiobis （Sulfosuccinimidylpro-琼脂糖

发表于由

【简单介绍】

货号：21578，名称：DTSSP, 3,3′-Dithiobis (Sulfosuccinimidylpropionate)，品牌：PIERCE，规格：50 MG

【详细说明】

订货：，，

 

DTSSP, 3,3′-Dithiobis （Sulfosuccinimidylpropionate）介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

DTSSP, 3,3′-Dithiobis （Sulfosuccinimidylpropionate）现货供应

 

PIERCE

21578

DTSSP, 3,3′-Dithiobis （Sulfosuccinimidylpropionate）

50 MG

PIERCE

21580

BS3, Bis（Sulfosuccinimidyl） Suberate

50 MG

PIERCE

21585

BS3, No-Weigh（tm） Format,     8 x 2 mg microtubes

8 X 2 MG

 

DTSSP, 3,3′-Dithiobis （Sulfosuccinimidylpropionate）品牌介绍

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括：

1）化学发光体和比色底物

 

2）分析试剂

 

3）荧光标记探针

 

4）酶联免疫分析试剂盒等

 

DTSSP, 3,3′-Dithiobis （Sulfosuccinimidylpropionate）仅用于科研

Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

3,3´-Dithiobis（sulfosuccinimidylpropionate）  （DTSSP） contains an amine-reactive N-hydroxysulfosuccinimide （sulfo-NHS） ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker. BS3, the non-cleavable analog of DTSSP is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

DTSSP Highlights

  • Amine reactive sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
  • Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
  • Cleavable crosslinker allows separation of crosslinked products
  • DTSSP crosslinker is not membrane permeable, so cell-surface crosslinking can be done