21857-PIERCE，N-Succinimidyl 3-（2-Pyridyldithio）Propionat-琼脂糖

发表于由

【简单介绍】

货号：21857，名称：N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP），品牌：PIERCE，规格：50 MG

【详细说明】

订货：，，

：  787994648

N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP）介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP）现货供应

PIERCE

21857

N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP）

50 MG

PIERCE

22122

Sulfo-SMCC, 1 g.

1 G

PIERCE

22305

MPBH: 4-（4-N-Maleimidophenyl）Butyric Acid Hydrazide Hydrochl

50 MG.

N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP）品牌介绍

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括：

1）化学发光体和比色底物

 

2）分析试剂

 

3）荧光标记探针

 

4）酶联免疫分析试剂盒等

N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP）

仅用于科研

Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable （reducible） disulfide bonds with cysteine sulfhydryls.

Thermo Scientific Pierce LC-SPDP is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable （reducible） disulfide bonds with cysteine sulfhydryls.

We offer three analogs of SPDP: the standard version （SPDP）, a derivative with a longer spacer arm （LC-SPDP） and Sulfo-LC-SPDP, the sulfonated analog of LC-SPDP is available for applications that require a water-soluble, membrane-impermeable crosslinker. SPDP （N -Succinimidyl 3-（2-pyridyldithio）-propionate） and LC-SPDP （Succinimidyl 6-（3-[2-pyridyldithio]-propionamido）hexanoate） are heterobifunctional, thiol-cleavable and membrane permeable crosslinkers. They each contain an amine-reactive N-hydroxysuccinimide （NHS） ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.

Highlights:

  • Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
  • Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
  • Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
  • Cleavable crosslinker allows separation of crosslinked products
  • SPDP crosslinker is membrane permeable, so crosslinking can be carried out inside the cell