22322-PIERCE，（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMID-琼脂糖

发表于由

【简单介绍】

货号：22322，名称：（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA ，品牌：PIERCE，规格：50 MG.
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【详细说明】

订货：，，

 787994648

（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA

PIERCE

22322

（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA

50 MG

PIERCE

22324

Sulfo-GMBS, N-gamma-maleimidobutyryloxysulfosuccinimide Este

50 MG

PIERCE

22330

1,6-BISMALEIMIDOHEXANE, BMH

50 MG

（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA品牌介绍

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 主要产品包括：

1）化学发光体和比色底物

 2）分析试剂

 3）荧光标记探针

 4）酶联免疫分析试剂盒等

（SULFO-SMCC）SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA用于科研

Thermo Scientific Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm （8.3 angstroms）.

Sulfosuccinimidyl-4-（N-maleimidomethyl）cyclohexane-1-carboxylate （Sulfo-SMCC） is a water-soluble, non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide （NHS ester） and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ~10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.

Sulfo-SMCC Highlights

  • Amine reactive Sulfo-NHS ester crosslinks rapidly with primary amine-containing molecule
  • Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
  • Sulfo-SMCC is water soluble, so crosslinking can be done in physiologic solutions
  • High purity, crystalline Sulfo-SMCC can be used to create high-purity maleimide-activated derivatives
  • Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.