22981-PIERCE,1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMI-琼脂糖

发表于由

【简单介绍】

货号：22981，名称： 1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCL （EDC） ，规格：25 GM

【详细说明】

订货：，，

1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCL   （EDC）介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCL   （EDC）现货供应

PIERCE

22981

1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCL   （EDC）

25 GM

PIERCE

23011

（MMTS） Methyl methanethiolsulfonate, 200 mg.

200 MG

PIERCE

23030

N-ETHYLMALEIMIDE

25 GM

1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCL   （EDC）品牌介绍

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括：

1）化学发光体和比色底物

 

2）分析试剂

 

3）荧光标记探针

 

4）酶联免疫分析试剂盒等

1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCL   （EDC）仅用于科研

Thermo Scientific Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.

1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride （EDC or EDAC） is a zero-length crosslinking agent used to couple carboxyl groups to primary amines. This crosslinker has been used in diverse applications such as forming amide bonds in peptide synthesis, attaching haptens to carrier proteins to form immunogens, labeling nucleic acids through 5’ phosphate groups and creating amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide （Sulfo-NHS）, EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding Sulfo-NHS.

Highlights:

  • Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS
  • By combining EDC and Sulfo-NHS, amine reactive Sulfo-NHS esters can be created on any carboxyl-containing molecule
  • Amide bonds formed with this crosslinking reaction provide a neutral linkage
  • Excess reagent and crosslinking byproducts are easily removed by washing with water or dilute acid
  • EDC is water soluble, so crosslinking can be done in physiologic solutions without adding organic solvent
  • High purity, crystalline EDC can be used to create high-purity activated derivatives