Thermo Scientific Pierce Plus Activated Peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins!
Sugar groups on native HRP have been oxidized with periodate to produce amine-reactive aldehydes. The activated peroxidase spontaneously and efficiently crosslink with primary amines on antibody or other proteins. The method is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and conjugate inactivation.
This high-quality prepared enzyme eliminates the difficulties inherent in preparing and validating activated peroxidase from scratch. The kit provides the enzyme, accessory reagents and protocol to easily produce high-performance conjugates for Western blotting, ELISA and other detection techniques.
Highlights:
- Reacts with readily accessible primary amines to form covalent secondary amine bonds with antibodies or other proteins
- Provides more efficient and stable crosslinking than glutaraldehyde methods, resulting in conjugates having higher activity and overall performance in most applications
- Can be stored for at least 12 months at -20°C
- Enzyme activity is 120 to 200 units/mg
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