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Lumi-Phos ™ WB, 100 mL 介绍
Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。
Lumi-Phos ™ WB, 100 mL 现货供应
PIERCE
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34150
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Lumi-Phos ™ WB, 100 mL.
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100 ML
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PIERCE
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35035
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Pierce Fast Semi-Dry Transfer Buffer, 10X Methanol-free
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PIERCE
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35035
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Pierce Fast Semi-Dry Transfer Buffer, 10X Methanol-free
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500 ML
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Lumi-Phos ™ WB, 100 mL 品牌介绍
美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html
主要产品包括:
1)化学发光体和比色底物
2)分析试剂
3)荧光标记探针
4)酶联免疫分析试剂盒等
Lumi-Phos ™ WB, 100 mL 仅用于科研
Thermo Scientific Lumi-Phos WB Substrate is a chemiluminescent substrate for sensitive detection of alkaline phosphatase activity from AP-conjugated antibodies and probes in Western blotting.
Lumi-Phos WB Chemiluminescent Substrate overcomes the limitations posed by conventional chemiluminescent substrates for alkaline phosphatase (AP). Lumi-Phos WB Substrate provides Western blot detection of low-picogram (attomole) levels of protein targets, which is comparable in sensitivity to many ECL substrates for horseradish peroxidase (HRP). Lumi-Phos WB Substrate also produces less background noise than other commercially available alk-phos chemiluminescent substrates, providing a better signal:noise ratio and a clearer image when exposed to autoradiography film or CCD imagers. Because signal generation is immediate, there’s no need to wait 15 to 30 minutes for a measurable signal. All of these benefits are combined with an affordable price.
Highlights:
- Alk-phos substrate – a chemiluminescent substrate for detection of alkaline phosphatase (AP or alk-phos) activity from antibodies and other Western blot probes
- Highly sensitive – detect low-picogram amounts of protein targets; detected 1.2pg (71 attomoles) of mouse IL-2 in example experiment
- Low background – provides high signal:noise ratios to yield clear blots that are easily documented by exposure to x-ray film or imagers
- Inexpensive – less costly to use than CDP-Star Substrate (based on 2008 U.S. list prices), and there is no need to purchase additional enhancers for nitrocellulose membranes
- Long signal duration – when conditions are optimized, blots generate usable signal for up to 8 hours (Em-max equals 440nm)
- Immediate strong signal – no more waiting 15 to 30 minutes for the signal to become strong enough to detect with imagers
- Ready to use – no mixing required with this one-component system
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