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IgM Purification Kit 介绍
Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。
IgM Purification Kit 现货供应
PIERCE
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44897
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IgM Purification Kit
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EA
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PIERCE
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44985
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Pierce Fab Preparation Kit, 10 rnxs, (0.25-4mg Ab per rxn)
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KIT
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PIERCE
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44988
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Pierce F(ab’)2 Preparation Kit, 10 rnxs,
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KIT
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IgM Purification Kit 品牌介绍
美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html
主要产品包括:
1)化学发光体和比色底物
2)分析试剂
3)荧光标记探针
4)酶联免疫分析试剂盒等
IgM Purification Kit 仅用于科研
The Thermo Scientific Pierce IgM Purification Kit is an easy-to-use affinity matrix and buffer system to purify IgM from mouse ascites fluid; the method uses immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.
Pierce MBP Agarose is purified mannan binding protein (also called mannose-binding lectin, MBL) that has been immobilized on 4% beaded agarose. When used with an optimized buffer system based on temperature- and calcium-dependent binding, this resin enables specific affinity purification of mouse IgM from ascites fluid and other concentrated samples. The resin and essential buffers are available individually or bundled together in a complete IgM Purification Kit containing a pre-packed 5mL column of the MBP Agarose. Immobilized mannan binding protein also is available as Thermo Scientific UltraLink Resin, a durable polyacrylamide support that is compatible with high flow rates and pressures.
Highlights:
- Immobilized MBP – affinity system based on mannan binding protein (MBP) allows specific purification of mouse IgM from ascites fluid or partial isolation of human IgM from serum
- Two resin options – choose 4% beaded agarose resin or polyacrylamide-based UltraLink Resin format to suit your chromatography system
- Complete kit – IgM Purification Kit includes a pre-packed MBP column and ready-to-use buffers for convenient purification of at least 1.5mg IgM from 0.5mL ascites samples per column-use
- Mild conditions – nondenaturing, near-neutral buffer conditions ensure recovery of functional antibodies
- Reusable – stable ligand and resins can be regenerated and reused at least 10 times without significant decline in binding capacity
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