Mouse anti-VWF Monoclonal Antibody(907CT12.1.9)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab) |
| 别名 |
VWF抗体;von Willebrand factor; vWF; von Willebrand antigen 2; von Willebrand antigen II; VWF; F8VWF
|
| 宿主 |
Mouse
|
| 特异性 |
This antibody is generated from a mouse immunized with .VWF recombinant protein.
|
| 反应种属 |
Human
|
| 应用 |
IHC-P~~1:25
FC~~1:100 WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 309kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
907CT12.1.9
|
| 同种型 |
IgG1
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Cancer;Cardiovascular;Signal Transduction
|
| 靶标 | |
| 背景说明 |
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
|
| 细胞定位 |
Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules
|
| UniProt |
P04275
|
| 参考文献 | |
| 参考文献 |
Bonthron D.,et al.Nucleic Acids Res. 14:7125-7128(1986).
Mancuso D.J.,et al.J. Biol. Chem. 264:19514-19527(1989). Scherer S.E.,et al.Nature 440:346-351(2006). Verweij C.L.,et al.EMBO J. 5:1839-1847(1986). Verweij C.L.,et al.EMBO J. 5:3074-3074(1986). |
实验结果图
Immunohistochemical analysis of paraffin-embedded H. kidney section using VWF(Cat#JP100459). JP100459 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of K562 cells using VWF(green, Cat#JP100459) compared to an isotype control of mouse IgG1(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysate from VWF protein, using VWF Antibody(Cat. #JP100459). JP100459 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35μg.