Anti-SLC40A1 Reference Antibody(LY2928057)

Anti-SLC40A1 Reference Antibody(LY2928057)描述宿主反应种属应用分子量纯化方法类型同种型储存/保存方法背景说明

概述
描述
内毒素:<1 eu>纯度:>95%
宿主
CHO
反应种属
Human
应用
ELISA, Kinetics (BLI), Kinetics (SPR), Flow Cyt, Functional assay
分子量
145.5 kDa
性能
纯化方法
Protein A purified
类型
Monoclonal Antibody
同种型
IgG
储存/保存方法
Store at -80℃ for one year.
靶标
背景说明
Anti-SLC40A1 Reference Antibody(LY2928057) is expressed from CHO. It has a predicted MW of 145.5 kDa.

MLN2238(Ixazomib)

MLN2238(Ixazomib)CAS号: 1072833-77-2分子式: C14H19BCl2N2O4分子量: 361.0 描述纯度储存/保存方法别名外观可溶性/溶解性靶点In vitro(体外研究)In vivo(体内研究)

产品描述
描述

MLN2238 inhibits the chymotrypsin-like proteolytic (β5) site of the 20S proteasome with IC50 and Ki of 3.4 nM and 0.93 nM in cell-free assays, respectively, also inhibits the caspase-like (β1) and trypsin-like (β2) proteolytic sites, with IC50 of 31 and 3500 nM. Phase 3.

纯度
≥98%
储存/保存方法
Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.
基本信息
别名
Ixazomib;MLN 2238;MLN-2238
外观
白色至类白色粉末
可溶性/溶解性
DMSO : 67 mg/mL (185.6 mM)

Ethanol : 9 mg/mL (24.92 mM)

生物活性
靶点
20S proteasome
In vitro(体外研究)
At higher concentrations, MLN2238 also inhibits the caspase-like (β1) and trypsin-like (β2) proteolytic sites with IC50 of 31nM and 3.5uM, respectively. MLN2238 inhibits Calu-6 cell with IC50 of 9.7 nM. MLN2238 is a selective, potent, and reversible inhibitor of the proteasome in tumor cells. MLN2238 shows time-dependent reversible proteasome inhibition. Both MLN2238 and Bortezomib shows time-dependent reversible proteasome inhibition; however, the proteasome dissociation half-life for MLN2238 is determined to be ∼6-fold faster than that of Bortezomib (18 and 110 minutes, respectively). MLN2238 dissociates more rapidly from the proteasome than Bortezomib, consistent with faster recovery of proteasome activity observed in the Proteasome-Glo assay. MLN2238 has a greater overall tumor pharmacodynamic effect than Bortezomib as assessed by 20S inhibition. MLN2238 is the biologically active form of MLN9708.
In vivo(体内研究)
MLN2238 induces a greater pharmacodynamic response than Bortezomib in xenograft tumors. MLN2238 shows greater maximum and sustained tumor proteasome inhibition compared with Bortezomib in xenograft models. These results confirm that the improved tumor exposure seen with MLN2238 translates into an improved tumor pharmacodynamic response both at the level of and downstream from the proteasome. MLN2238 shows antitumor activity in the CWR22 xenograft model. MLN2238 shows greater tumor pharmacodynamic responses in WSU-DLCL2 xenografts compared with Bortezomib. Similarly, Bortezomib treatment only led to a minor increase in GADD34 levels in WSU-DLCL2 xenograft tumors, whereas MLN2238 strongly induces its expression. MLN2238 has an improved pharmacodynamic profile and antitumor activity compared with Bortezomib in both OCI-Ly10 and PHTX22L models.

分子结构图

MLN2238(Ixazomib)

LY-2624803

LY-2624803CAS号: 879409-35-5分子式: C22H25N3O3分子量: 379.45描述纯度储存/保存方法

产品描述
描述
LY-2624803 is a 5-HT2A receptor antagonist and histamine receptor inverse agonist that can be used to improve slow wave sleep.
纯度
98%
储存/保存方法
Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.

AZD-9291 mesylate (Osimertinib,Mereletinib)

AZD-9291 mesylate (Osimertinib,Mereletinib)CAS号: 1421373-66-1分子式: C29H37N7O5S分子量: 595.71描述纯度储存/保存方法别名外观可溶性/溶解性靶点In vivo(体内研究)

产品描述
描述

AZD-9291 is a potent and selective mutated forms EGFR inhibitor(Exon 19 deletion EGFR IC50=12.92 nM, L858R/T790M EGFR IC50= 11.44 nM, wild type EGFR IC50= 493.8 nM).

纯度
>99%
储存/保存方法
Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.
基本信息
别名
Mereletinib mesylate; EGFR inhibitor; AZD9291 mesylate; AZD 9291 mesylate,Tagrisso
外观
Powder
可溶性/溶解性
DMSO
生物活性
靶点
EGFR
In vivo(体内研究)
The tumor-bearing mice are treated with AZD-9291 (5 mg/kg/day) for one to two weeks. Within days of treatment, 5 of 5 C/L858R mice displays nearly 80% reduction in tumor volume by magnetic resonance imaging MRI after therapy with AZD-9291, while 5 of 5 mice treated with vehicle shows tumor growth. AZD-9291 demonstrates improved rat PK, reduced hERG affinity, and improved IGF1R margins relative to the previously described compounds, and so this compound is selected for further investigation. AZD-9291 also offers an additional degree of broader chemical and profile diversity when compared to the previously described lead compounds. Upon dosing AZD-9291 in three efficacy models, The comparable efficacy is observed at relatively low doses (10 mg/kg per day). The excellent efficacy is also observed when AZD-9291 is dosed at 5 mg/kg per day.

分子结构图

AZD-9291 mesylate (Osimertinib,Mereletinib)

ML-241

ML-241CAS号: 1346528-06-0分子式: C23H24N4O分子量: 372.46描述纯度储存/保存方法别名外观可溶性/溶解性

产品描述
描述
ML241 is a potent, selective, and reversible inhibitor of the AAA ATPase p97 (IC50=100 nM). It blocks p97-dependent proteasome substrate with an IC50 of 3500 nM.
纯度
>98%
储存/保存方法
Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.
基本信息
别名
ML241,ML 241
外观
Powder
可溶性/溶解性
10 mM in DMSO

分子结构图

ML-241

Mouse anti-P100 Monoclonal Antibody(19AT953.286.93)

Mouse anti-P100 Monoclonal Antibody(19AT953.286.93)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献

概述
描述
Purified Mouse Monoclonal Antibody (Mab)
别名
P100抗体;Targeting protein for Xklp2; Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; Protein fls353; Restricted expression proliferation-associated protein 100; p100; TPX2; C20orf1; C20orf2; DIL2; HCA519
宿主
Mouse
特异性
This monoclonal antibody is generated from mice immunized with a collection of antigenic peptides from different regions of P100.
反应种属
Human, Mouse
应用
WB~~1:100~500
分子量
Predicted molecular weight: 86kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
性能
类型
Monoclonal Antibody
克隆号
19AT953.286.93
同种型
Mouse IgG1
储存/保存方法
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
存储溶液
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
研究领域
Cancer;Cell Biology
靶标
背景说明
Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at ‘Thr-288’ and protects this residue against dephosphorylation.
细胞定位
Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules
UniProt
Q9ULW0
参考文献
参考文献
Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.
A cancer-associated aurora A mutant is mislocalized and misregulated due to loss of interaction with TPX2. Bibby RA, et al. J Biol Chem, 2009 Nov 27. PMID 19801554.
Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity. Hosgood HD 3rd, et al. Occup Environ Med, 2009 Dec. PMID 19773279.
Overexpression of the receptor for hyaluronan-mediated motility, correlates with expression of microtubule-associated protein in human oral squamous cell carcinomas. Shigeishi H, et al. Int J Oncol, 2009 Jun. PMID 19424574.
Dynamic release of nuclear RanGTP triggers TPX2-dependent microtubule assembly during the apoptotic execution phase. Moss DK, et al. J Cell Sci, 2009 Mar 1. PMID 19208764.

实验结果图

Mouse anti-P100 Monoclonal Antibody(19AT953.286.93)

Western blot analysis of lysates from HT29, Jurkat, MCF-7 cell line (from left to right), using P100 Antibody(19AT953. 286. 93). 19AT953. 286. 93 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Mouse anti-P100 Monoclonal Antibody(19AT953.286.93)

Western analysis of extracts from Jurkat cells using P100 antibody.

Rabbit anti-Synaptotagmin(pT202/199) Polyclonal Antibody

Rabbit anti-Synaptotagmin(pT202/199) Polyclonal Antibody描述别名宿主特异性反应种属应用分子量免疫原纯化方法类型储存/保存方法UniProt

概述
描述
Rabbit polyclonal antibody to Synaptotagmin (pT202/199)
别名
Synaptotagmin抗体;SYT1; SVP65; SYT; Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT2; Synaptotagmin-2; Synaptotagmin II; SytII
宿主
Rabbit
特异性
Recognizes endogenous levels of Synaptotagmin (pT202/199) protein.
反应种属
Human, Mouse, Rat
应用
WB 1:500 – 1:1000, IHC 1:50 – 1:100
分子量
Detects a band of approximately 47kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Synaptotagmin. The exact sequence is proprietary.
性能
纯化方法
The antibody was purified by immunogen affinity chromatography.
类型
Polyclonal Antibody
储存/保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
靶标
UniProt
P21579

实验结果图

Rabbit anti-Synaptotagmin(pT202/199) Polyclonal Antibody

Western blot analysis of Synaptotagmin (pT202/199) expression in K562 (A), HEK293T (B), U87MG (C), A549 (D) whole cell lysates.

Rabbit anti-Synaptotagmin(pT202/199) Polyclonal Antibody

Immunohistochemical analysis of Synaptotagmin (pT202/199) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

JPH203

JPH203CAS号: 1037592-40-7分子式: C23H19Cl2N3O4分子量: 472.32描述纯度储存/保存方法别名外观可溶性/溶解性靶点In vitro(体外研究)In vivo(体内研究)

产品描述
描述

JPH203 is a potent and selective L-type amino acid transporter 1 (LAT-1) inhibitor. 

纯度
>98%
储存/保存方法
Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.
基本信息
别名
KYT-0353;JPH-203;JPH 203
外观
light-yellow to off-white solid
可溶性/溶解性
0.1N hydrochloric acid : 7.3 mg/mL (15.46 mM; Need ultrasonic and warming)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

生物活性
靶点
LAT1
In vitro(体外研究)
JPH203 is a selective inhibitor of LAT1. JPH203 (KYT-0353) inhibits 14C-leucine uptake in S2-hLAT1 and HT-29 cells, with IC50s of 0.14 μM and 0.06 μM. JPH203 (3-1000 μM) exhibits concentration-dependent inhibitory effects on S2-hLAT1 cell growth with an IC50 of 16.4 μM. JPH203 also displays inhibitory activities against HT-29 cell growth, with an IC50 value of 4.1 μM. JPH203 (0.001-100 μM) inhibits the 14C-leucine (1.0 μM) uptake in a concentration dependent way by the YD-38 cells with an IC50 value of 0.79 ± 0.06 μM. JPH203 slightly shows such effects in normal human oral keratinocytes (NHOKs). JPH203 (0.01-30 mM, 1-4 d) completely inhibits the proliferation of YD-38 cells in a dose- and time-dependent manner. However, JPH203 slightly inhibits the proliferation of NHOKs. JPH203 (30 mM) induces apoptosis of YD-38 cells. JPH203 (3 mM) also increases the level of cleaved PARP in activation of the caspases cascade. JPH203 (30 mM) induces mitochondria-dependent apoptosis in Saos2 human osteosarcoma cells. JPH203 (0.001-100 µM) inhibits 14C-leucine (1.0 µM) uptake slightly in FOB cells with an IC50 value of 92.12 ± 10.71 µM, but potently exihibts such effects in Saos2 cells with an IC50 value of 1.31 ± 0.27 µM. JPH203 (0.01 to 30 mM, 1-4 d) potently inhibits cell proliferation in Saos2 cells in a dose- and time-dependent manner, with an IC50 of 4.09-0.09 mM, but slightly inhibits that of FOB cells, with an IC50 of 24.1-2.8 mM.
In vivo(体内研究)
JPH203 (6.3, 12.5, and 25.0 mg/kg, i.v. for 14 days) exhibits dose-dependent inhibition on HT-29 tumor growth in nude mice.

分子结构图

JPH203

Rabbit Anti-Phospho-S6 Ribosomal Protein(Ser240/Ser244) Monoclonal Antibody(JRMR-259)

Rabbit Anti-Phospho-S6 Ribosomal Protein(Ser240/Ser244) Monoclonal Antibody(JRMR-259)别名宿主反应种属应用分子量纯化方法类型克隆号同种型储存/保存方法背景说明细胞定位UniProt

概述
别名
Phospho-S6 Ribosomal Protein抗体;Phosphoprotein NP33; RPS6
宿主
Rabbit
反应种属
Human, Mouse, Rat
应用
WB:1:500-1:1000,IHC-P:1:200-1:1000,ICC/IF:1:50
分子量
Predicted molecular weight: 29kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
性能
纯化方法
Protein A
类型
Monoclonal Antibody
克隆号
JRMR-259
同种型
IgG
储存/保存方法
储存温度:-20℃,避免反复冻融
靶标
背景说明
40S小核糖体亚基的组成部分。 通过选择性翻译特定类别的mRNA在控制细胞生长和增殖中起重要作用。
细胞定位
胞质溶胶,内质网,核
UniProt
P62753