BIX-01294 trihydrochlorideCAS号: 1392399-03-9分子式: C28H38N6O2·3HCl分子量: 600.02描述纯度储存/保存方法别名可溶性/溶解性靶点In vitro(体外研究)参考文献
产品描述 | |
描述 |
BIX-01294 trihydrochloride是一种G9a histone methyltransferase抑制剂,无细胞试验中IC50为2.7 μM,降低大部分组蛋白H3K9me2,也微弱抑制GLP(主要是H3K9me3),对其他组蛋白甲基转移酶没有显著的抑制活性。 |
纯度 |
>98%
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储存/保存方法 |
Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.
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基本信息 | |
别名 |
BIX 01294
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可溶性/溶解性 |
DMSO: 90 mg/mL ; Water: 90 mg/mL
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生物活性 | |
靶点 |
G9a
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In vitro(体外研究) |
BIX01294 is a selective inhibitor of G9a histone methyltransferase and does not affect SUV39H1(H320R) and PRMT1 within the tested concentration range. BIX-01294 specifically inhibited G9a (H3K9me2) and, to a lesser extent, the closely related GLP enzyme (primarily H3K9me3), with an IC50 of 1.7 μM for G9a and 38 μM for GLP. BIX-01294 inhibits G9a in an uncompetitive manner with SAM. BIX-01294 (4.1μM) reduces H3K9me2 Levels in Bulk Histone Preparations from wt ES cells, mouse embryonic fibroblasts and HeLa cells, but not in G9a deficient stem cells. BIX-01294 is a valuable inhibitor for the transient modulation of chromosomal H3K9me2. BIX-01294 Reduces H3K9me2 at Several G9a Target Genes including Bim1 and Serac1. BIX01294 could reactivate expression of HIV-1 from latently infected cells such as ACH-2 and OM10.1.
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参考文献 | |
参考文献 |
[1] Kubicek S, et al. Mol Cell, 2007, 25(3), 473-481. [2] Imai K, et al. J Biol Chem, 2010, 285(22), 16538-16545. |
分子结构图