维生素C[L-抗坏血酸]检测试剂盒 L-Ascorbic Acid (L-Ascorbate) 货号：K-ASCO Megazyme中文站

发表于由

分析物意义：  蔬菜水果中的天然成分或加工食品中的添加成分

Megazyme检测试剂盒优点：反应快、试剂稳定 

For the specific assay of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products. Content:40 assays per kit

Colourimetric method for the determination of L-Ascorbic Acid 
in foodstuffs, feed, wine and other materials

Principle:
                        (5-methylphenazinium methosulphate)
(1) L-Ascorbic acid + R-H2 + MTT → dehydroascorbate +                                                                                         MTT-formazan + H+
                            (ascorbic acid oxidase)
(2) L-Ascorbic acid + ½O2 → dehydroascorbate + H2O

Kit size:                             40 assays (manual) / 400 (microplate) 
                                          / 400 (auto-analyser)
Method:                             Spectrophotometric at 578 nm
Reaction time:                    ~ 8 min
Detection limit:                   0.175 mg/L
Application examples: 
Wine, beer, fruit juices, soft drinks, jam, milk, dairy products 
(e.g. cheese), dietetic foods, baby foods, processed meat, baking
additives, fruit and vegetables (e.g. tomato and potato), 
pharmaceuticals, feed and other materials (e.g. biological cultures,  
samples, etc.)
Method recognition:     
Methods based on this principle have been accepted by MEBAK

Advantages

  • Very competitive price (cost per test)
     
  • All reagents stable for > 6 months after preparation
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats 

 

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