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Pierce Rapid ELISA Mouse mAB Isotyping Kit 介绍
Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。
Pierce Rapid ELISA Mouse mAB Isotyping Kit 现货供应
PIERCE
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37503
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Pierce Rapid ELISA Mouse mAB Isotyping Kit
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PIERCE
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37503
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Pierce Rapid ELISA Mouse mAB Isotyping Kit
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KIT
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PIERCE
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37515
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SuperBlock Blocking Buffer in PBS, 1 liter
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1 LITER
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Pierce Rapid ELISA Mouse mAB Isotyping Kit 品牌介绍
美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html
主要产品包括:
1)化学发光体和比色底物
2)分析试剂
3)荧光标记探针
4)酶联免疫分析试剂盒等
Pierce Rapid ELISA Mouse mAB Isotyping Kit 仅用于科研
The Thermo Scientific Pierce Rapid ELISA Mouse mAb Isotyping Kit provides antigen-independent determination of mouse monoclonal antibody isotype using a microplate method that enables multiple samples to be tested in about one hour.
This fast assay uses ELISA strip-plates that are pre-coated in different wells with anti-mouse heavy-chain capture antibodies (anti-IgG1, IgG2a, IgG2b, IgG3, IgA and IgM) or anti-mouse light-chain capture antibody (kappa or lambda). This approach eliminates the need to purify and immobilize an antigen to determine immunoglobulin isotype (antibody subclass and light chain identity). A mouse monoclonal antibody sample applied to the wells can be isotyped within one hour. Results are evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450nm.
Highlights:
- Fast – determine antibody isotype in approx. 1 hour; considerably faster than traditional ELISA kits
- Convenient – eight-well strip format allows partial use of the plate; use one strip (column) for each sample (12 samples per plate)
- Specific – characterize antibodies for six different subclasses and two different light-chain types
- Sensitive – accurate characterization with samples containing at least 3ng/mL of test antibody
- Flexible – kit is compatible with hybridoma cell culture supernatant, ascites fluid or purified antibodies
- Cost effective – cost per assay is less than with single-use assay strips or cassettes
- No special equipment needed – assess results visually or measure quantitatively using an ordinary ELISA plate reader (450nm)
- Complete and easy to use – includes precoated plates, detecting antibody, buffers, and TMB substrate and stop solutions
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