Recombinant human PRKCB protein
货号：
P03728
品牌：
Jinpan
产品简介
| 基因名 | PRKCB |
| 英文全称 | protein kinase C, beta |
| 别称 | PKC-beta;PKCB;PRKCB1;PRKCB2 |
| 中文名称 | 蛋白激酶C,β型 |
| Uniprot | P05771 |
| GeneID | 5579 |
| 交叉反应(推测) | Human Mouse Rat (Bovine Rabbit Zebrafish) |
| 氨基酸大小 | 38kDa |
| 表达氨基酸 | 324-673aa |
| Background | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation.Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation.Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at ‘Ser-559’, ‘Ser-644’ and ‘Ser-652’.Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1.Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at ‘Ser-180’, which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity.Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates ‘Ser-36’ of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer.Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of ‘Thr-6’ of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 ‘Lys-4’ (H3K4me) by LSD1/KDM1A.In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade.Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4.Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1.Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression.In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction.Also involved in triglyceride homeostasis.Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. |
| Application | Immunology research |
| Derived from | E.coli |
| Purification | NI-NTA affinity purification |
| Formulation | Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0. |
| Shipping | The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| Storage | Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years. |
| Reconstitution | Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Purity | Purity≥85% as determined by reducing SDS-PAGE. |
| Endotoxin | / |
| Bioactive | Unknown. (Ban’t be used in biological activity experiments such as cell culture, treatment and diagnosis.) |
| 规格 | 10ug 50ug 100ug 1mg |