Podophyllotoxin 鬼臼毒素

Podophyllotoxin 鬼臼毒素

货号:
IP0310

品牌:
Jinpan

Podophyllotoxin  鬼臼毒素

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产品简介
EC EINECS 208-250-4
MDL MFCD00075290
别名 Podofilox; Podophyllotoxin (8CI)
CAS 518-28-5
分子式 C22H22O8
分子量 414.41
纯度 HPLC≥98%
单位
SMILES O=C1OC[C@]2([H])[C@@H](O)C3=C(C=C4OCOC4=C3)[C@@H](C5=CC(OC)=C(OC)C(OC)=C5)[C@]21[H]
靶点 Topo II;Microtubule/Tubulin
规格 10mg 10mM*1mL (in DMSO) 50mg

是DNA拓扑异构酶II和微管组装抑制剂。

使用本产品的应用案例(仅供参考

In Vitro

Cell(MB-231 and MDA-MB-468 cells),0.75~24 mmol/mL podophyllotoxin,48h

The effect of podophyllotoxin(purity:≥ 98%, Jinpan, China) on cell viability was determined by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, for which MDA MB-231 and MDA-MB-468 cells were cultured in 96-well plates. The density of MDA-MB-231 cells was 1.2 × 104 per well, while that of MDA-MB-468 cells was 1.5 × 104 cells per well. Before adding the drug, the cells were cultured in a serumfree medium for 6h and treated with varying concentrations of podophyllotoxin (0.75, 1.5, 3, 6, 12, and 24 mmol/ml) for 48 h. After that, each well was incubated with 15 ml of MTT for 4 h, and 150-ml dimethyl sulfoxide was added after incubation. After shaking, the microplate reader was used to detect the absorbance at 570 nm. 

来源文献:Zhang W, Liu C, Li J, Liu R, Zhuang J, Feng F, Yao Y, Sun C. Target Analysis and Mechanism of Podophyllotoxin in the Treatment of Triple-Negative Breast Cancer. Front Pharmacol. 2020 Aug 7;11:1211. doi: 10.3389/fphar.2020.01211. PMID: 32848800; PMCID: PMC7427588.