亮抑酶肽

亮抑酶肽

货号:
IL0070

品牌:
Jinpan

亮抑酶肽

暂无详情
产品简介
MDL MFCD00037012
EC EINECS 600-443-5
别名 亮抑蛋白酶肽;LeupeptinHemisulfate
英文名称 Leupeptin
CAS 103476-89-7
分子式 C20H38N6O4·1/2 H2SO4
分子量 475.59
纯度 ≥98%
单位
SMILES O=C[C@@H](NC([C@@H](NC([C@@H](NC(C)=O)CC(C)C)=O)CC(C)C)=O)CCCNC(N)=N.[0.5H2SO4]
靶点 Cathepsin;Serine Protease
规格 1mg 5mg 10mM*1mL (in DMSO) 10mM*1mL in Water

是一种可透过膜的巯基蛋白酶抑制剂,可抑制 Cathepsin B, Cathepsin H 和 Cathepsin L,并损害两性溶酶体的融合。

使用本产品的应用案例(仅供参考

In Vitro

Western blotting. (1 µM Leupeptin)

MCF-7 cells were treated with the aforementioned CoCl2 concentrations for 24 h and cells were lysed with ice-cold radioimmunoprecipitation assay buffer (150 mM NaCl, 1.0% NP-40, 0.1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS and 50 mM Tris-HCl, pH 8.0) and protease inhibitors (AEBSF at 2 mM, Aprotinin at 0.3 µM, Bestatin at 116 µM, E-64 at 14 µM, Leupeptin at 1 µM and EDTA at 1 mM; Beijing Jinpan Science & Technology Co., Ltd., Beijing, China)

来源文献:Li Q, Ma R, Zhang M. CoCl2 increases the expression of hypoxic markers HIF-1α, VEGF and CXCR4 in breast cancer MCF-7 cells. Oncol Lett. 2018 Jan;15(1):1119-1124. doi: 10.3892/ol.2017.7369. Epub 2017 Nov 8. PMID: 29391899; PMCID: PMC5769392.