Rabbit anti-5-HT Polyclonal Antibody别名宿主特异性反应种属应用分子量免疫原浓度纯化方法类型同种型储存/保存方法存储溶液研究领域背景说明UniProt
| 概述 | |
| 别名 |
5-HT抗体;Serotonin; 5-hydroxytryptamine; 3-(2-Aminoethyl)-5-hydroxyindole hydrochloride; 5 hydroxy tryptophan; 5 Hydroxytryptophan; 5HTP; 5-HT; 5-Hydroxytryptamine hydrochloride 5-羟色胺
|
| 宿主 |
Rabbit
|
| 特异性 |
5-HT
|
| 反应种属 |
Rat,
|
| 应用 |
ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
|
| 分子量 |
Predicted molecular weight: 0.21268kDa
|
| 免疫原 |
KLH conjugated to 5-HT:
|
| 性能 | |
| 浓度 |
1mg/ml
|
| 纯化方法 |
affinity purified by Protein A
|
| 类型 |
Polyclonal Antibody
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
|
| 研究领域 |
肿瘤 细胞生物 免疫学 神经生物学 药物及化合物
|
| 靶标 | |
| 背景说明 |
Serotonin (pronounced) (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesized in serotonergic neurons in the central nervous system (CNS) and enterochromaffin cells in the gastrointestinal tract of animals including humans. Serotonin is also found in many mushrooms and plants, including fruits and vegetables.
|
| UniProt |
N/A
|
实验结果图
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-5-HT Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining