Rabbit anti-Ep300 Polyclonal Antibody别名宿主特异性反应种属预测反应种属应用分子量免疫原浓度纯化方法类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt
| 概述 | |
| 别名 |
Ep300抗体;CREBBP/EP300 inhibitory protein 1; Cyclic AMP responsive enhancer binding protein; E1A associated protein p300; E1A binding protein p300; E1A-associated protein p300; EC 2.3.1.48; EP300; EP300: E1A binding protein p300; EP300_HUMAN; istone acetyltransferase p300; 300 HAT; RB and P300 binding protein EID 1; Retinoblastoma protein associated protein; RSTS2
|
| 宿主 |
Rabbit
|
| 特异性 |
Ep300
|
| 反应种属 |
Human, Mouse
|
| 预测反应种属 |
Rat;Dog;Pig;Cow;Horse;Sheep;
|
| 应用 |
ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
|
| 分子量 |
Predicted molecular weight: 264kDa
|
| 免疫原 |
KLH conjugated synthetic peptide derived from human KAT3B/p300 (1461-1520aa):1451-1600/2414
|
| 性能 | |
| 浓度 |
1mg/ml
|
| 纯化方法 |
affinity purified by Protein A
|
| 类型 |
Polyclonal Antibody
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
|
| 研究领域 |
肿瘤 细胞生物 信号转导 干细胞 转录调节因子 结合蛋白 新陈代谢 表观遗传学
|
| 靶标 | |
| 背景说明 |
Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.
|
| 细胞定位 |
细胞核 细胞浆
|
| UniProt |
Q09472
|