Rabbit anti-RPS6 Polyclonal Antibody(Ser240/244)描述别名宿主特异性反应种属预测反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明UniProt参考文献
| 概述 | |
| 描述 |
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
|
| 别名 |
RPS6抗体;40S ribosomal protein S6; Phosphoprotein NP33; RPS6
|
| 宿主 |
Rabbit
|
| 特异性 |
This RPS6 (Ser240/244) Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from human RPS6.
|
| 反应种属 |
Human
|
| 预测反应种属 |
B, C, Pr, R, X
|
| 应用 |
WB~~1:1000
IHC-P~~1:50~100 FC~~1:10~50 |
| 分子量 |
Predicted molecular weight: 29kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
| 研究领域 |
Cell Biology
|
| 靶标 | |
| 背景说明 |
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
|
| UniProt |
P62753
|
| 参考文献 | |
| 参考文献 |
Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
Fujita, K., et al. Acta Neuropathol. 116(4):439-445(2008) Robledo, S., et al. RNA 14(9):1918-1929(2008) Glover, E.I., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295 (2), R604-R610 (2008) : Ma, X.M., et al. Cell 133(2):303-313(2008) |
实验结果图
Western blot analysis of lysate from HeLa cell line, using RPS6 Antibody(Ser240/244)(Cat. #JP100640). JP100640 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
RPS6 antibody (Ser240/244) (Cat. #JP100640) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6 antibody (Ser240/244) for immunohistochemistry. Clinical relevance has not been evaluated.
RPS6 Antibody (Ser240/244) (Cat. #JP100640) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.