Rabbit anti-ZNF219 Polyclonal Antibody(N-term)描述别名宿主特异性反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Rabbit Polyclonal Antibody (Pab)
|
| 别名 |
ZNF219抗体;Zinc finger protein 219; ZNF219
|
| 宿主 |
Rabbit
|
| 特异性 |
This ZNF219 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human ZNF219.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:1000
IHC-P~~1:10~50 |
| 分子量 |
Predicted molecular weight: 77kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|
| 研究领域 |
Developmental Biology;Signal Transduction
|
| 靶标 | |
| 背景说明 |
May be involved in transcriptional regulation.
|
| 细胞定位 |
Nucleus.
|
| UniProt |
Q9P2Y4
|
| 参考文献 | |
| 参考文献 |
Olsen,J.V., Cell 127 (3), 635-648 (2006)
Paces-Fessy,M., Biochem. J. 378 (PT 2), 353-362 (2004) Sakai,T., DNA Res. 10 (4), 155-165 (2003) |
实验结果图
Western blot analysis of lysate from SH-SY5Y cell line, using ZNF219 Antibody (N-term)(Cat. #JP104077). JP104077 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot analysis of lysate from human heart tissue, using ZNF219 Antibody (N-term) (Cat. #JP104077). JP104077 was diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot analysis of anti-ZNF219 Antibody (N-term) (Cat.#JP104077) in 293 cell line lysates (35ug/lane).ZNF219(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human testis tissue reacted with ZNF219 antibody (N-term) (Cat.#JP104077), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.