6-氯嘌呤
货号：
IC2180
品牌：
Jinpan
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产品简介
| 有效期 | 2年 |
| 描述 | 是一种化合物，具有生物或化学活性。 |
| MDL | MFCD00075825 |
| EC | EINECS 201-745-6 |
| 别名 | 6-Chloro-9H-purine |
| 英文名称 | 6-Chloropurine |
| CAS | 87-42-3 |
| 分子式 | C5H3ClN4 |
| 分子量 | 154.56 |
| 储存条件 | 2-8℃ |
| 纯度 | ≥98% |
| 外观（性状） | Light yellow Powder |
| 单位 | 瓶 |
| 生物活性 | 6-Chloropurine is a building block in chemical synthesis. Antitumor activities.Furthermore, the induction of apoptosis was established and cell cycle analysis was accomplished demonstrating a G2/M cell cycle arrest.[1]. |
| In Vitro | 6-Chloro-7-(2,3,4,6-tetra-O-benzyl-b-D-galactopyranosyl) purine (3) and 6-chloro-9-(2,3,4,6-tetra-O-benzyl-b-D-galactopyranosyl)purine (4) were obtained by the reaction of methyl 2,3,4,6-tetra-O-benzyl a-D-galactopyranoside (280 mg, 0.50 mmol) and 6-chloropurine (122 mg, 0.75 mmol) according to the general procedure. Purification by column chromatography (ethyl acetate/ cyclohexane 1:1) afforded 3 (51 mg, 15%) and 4 (151 mg, 44%).[1] |
| SMILES | ClC1=C2NC=NC2=NC=N1 |
| 靶点 | Others |
| 细胞实验 | Cytotoxicity assay: The cytotoxicity of the compounds was evaluated using the sulforhodamine-B (SRB) microculture colorimetric assay. In short, exponentially growing cells were seeded into 96-well plates on day 0 at the appropriate cell densities to prevent confluence of the cells during the period of experiment. After 24 h, the cells were treated with serial dilutions of the compounds (0e100 mM) for 96 h. The final concentration of DMSO or DMF solvent never exceeded 0.5%, which was non-toxic to the cells. The percentages of surviving cells relative to untreated controls were determined 96 h after the beginning of drug exposure. After a 96 h treatment, the supernatant medium from the 96 well plates was discarded and the cells were fixed with 10% TCA. For a thorough fixation, the plates were allowed to rest at 4 _x005f C. After fixation, the cells were washed in a strip washer. The washing was done four times with water using alternate dispensing and aspiration procedures. The plates were then dyed with 100 ml of 0.4% SRB (sulforhodamine B) for about 20 min. After dying, the plates were washed with 1% acetic acid to remove the excess of the dye and allowed to air dry overnight. 100 ml of 10 mM Tris base solution were added to each well, and absorbance was measured at l ? 570 nm. [1] Cell cycle analysis: Approximately 1*106 cells (HT29, A2780 or NiH 3T3) were seeded in cell culture flasks (25 cm2), and the cells were allowed to grow for 24 h. After removing of the used medium, the substance loaded medium was reloaded (or a blank fresh medium as a control). After 24 or 48 h, the living cells were harvested, washed with PBS (with Mg2t and Ca2t) twice and ethanol fixed (70%, 4℃, 1 h). After removing of the fixation and permeabilization agent, the cells were washed with PBS buffer (with Mg2t and Ca2t, containing 1% BSA and 0.1% NaN3, 3×1 mL, 1000 rpm) and adjusted to 1*105 million cells. The pellet was gently suspended in staining buffer (PBS buffer containing BSA, RNAase, NaN3 and PI analog) and incubated for 30 min at 37 C. [1] |
| 数据来源文献 | [1] Schwarz S , Siewert B , Csuk R , et al. New antitumor 6-chloropurine nucleosides inducing apoptosis and G2/M cell cycle arrest[J]. European Journal of Medicinal Chemistry, 2015, 90:595-602. |
| 规格 | 100mg 250mg 500mg |