CA-5f
货号：
IC3160
品牌：
Jinpan
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产品简介
| CAS | 1370032-19-1 |
| 分子式 | C24H24N2O3 |
| 分子量 | 388.46 |
| 储存条件 | -20℃ |
| 纯度 | ≥98% |
| 单位 | 瓶 |
| 生物活性 | CA-5f是一种有效的自噬晚期（late-stage autophagy）抑制剂，通过抑制自噬体-溶酶体融合发挥作用。[1] |
| In Vitro | CA-5f neither impairs the hydrolytic function nor the quantity of lysosomes. Treatment of human umbilical vein endothelial cells (HUVECs) with CA-5f for 1 h suppresses the levels of cytoskeletal proteins and membrane traffic proteins. CA-5f exhibits strong cytotoxicity against A549 non-small cell lung cancer (NSCLC) cells, but low cytotoxicity to normal human umbilical vein endothelial cells (HUVECs), by increasing mitochondrial-derived reactive oxygen species (ROS) production. CA-5f inhibits autophagic flux by blocking autophagosome-lysosome fusion instead of affecting the pH and hydrolytic function of lysosomes[1]. |
| In Vivo | CA-5f effectively suppresses the growth of A549 lung cancer xenograft as a single agent with an excellent tolerance in vivo. It inhibits autophagic flux, induced apoptosis, and does not affect the level of CTSB (cathepsin B) and CTSD (cathepsin D) in vivo[1]. |
| SMILES | O=C(/C(CN(C)C/1)=C/C2=CNC3=C2C=CC=C3)C1=CC4=CC=C(OC)C(OC)=C4 |
| 靶点 | Autophagy |
| 动物实验 | Animal Models: Four-week-old BALB/c nude mice (subcutaneous injection of A549 cells at the right scapula); Dosages: 40 mg/kg; Administration: injected via caudal vein[1] |
| 细胞实验 | A549 cells or HUVECs are seeded into E-Plate 16-well plates overnight and then treated with DMSO or CA-5f and continuously monitored for 96 h. The changes of cell index can be used to monitor cell viability.[1] |
| 数据来源文献 | [1] Zhang L, et al. Autophagy. 2019, 15(3):391-406. |
| 规格 | 5mg 10mg |
CVT-313是有效的CDK2抑制剂。