Rabbit anti-Asialoglycoprotein Receptor 1 Recombinant Monoclonal Antibody(292-48)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
ASGP-R 1; ASGPR 1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1; ASGR1; CLEC4H1
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB: 1:1000-1:5000, IHC-P: 1:2000
|
| 免疫原 |
Recombinant protein
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
292-48
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Asialoglycoprotein receptors (ASGP-R; exist as subtypes 1 and 2) are located on the surface of hepatic cell membranes and are involved in the binding and endocytosis of glycoproteins that have exposed carbohydrate or N-acetylgalactosamine residues. In addition, the decreased level of ASGP-R on the hepatic cells has been shown to correlate clinically with the degree of liver function retained due to the development of cirrhosis, cancer, or viral hepatitis [PMID: 20641215].
|
| 细胞定位 |
Secreted, Membrane
|
| UniProt |
P07306
|
实验结果图
WB result of Asialoglycoprotein Receptor 1 Rabbit mAb Primary antibody: Asialoglycoprotein Receptor 1 Rabbit mAb at 1/5000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: HepG2 whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 33kDa Observed MW: 40~50kDa Exposure time: 5s
WB result of Asialoglycoprotein Receptor 1 Rabbit mAb Primary antibody: Asialoglycoprotein Receptor 1 Rabbit mAb at 1/5000 dilution Lane 1: mouse liver lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 33kDa Observed MW: 40kDa Exposure time: 30s
WB result of Asialoglycoprotein Receptor 1 Rabbit mAb Primary antibody: Asialoglycoprotein Receptor 1 Rabbit mAb at 1/1000 dilution Lane 1: rat liver lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 33kDa Observed MW: 40kDa Exposure time: 60s
IHC shows positive staining in paraffin-embedded human liver. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.