Rabbit anti-Progesterone Receptor Monoclonal Antibody(JRMR-205)别名宿主反应种属应用纯化方法类型克隆号同种型储存/保存方法背景说明细胞定位UniProt
| 概述 | |
| 别名 |
Progesterone Receptor 抗体;NR3C3; PGR; PR
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse
|
| 应用 |
IHC-P:1:200-1:1000;
|
| 性能 | |
| 纯化方法 |
Protein A
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
JRMR-205
|
| 同种型 |
IgG
|
| 储存/保存方法 |
储存温度：-20℃，避免反复冻融
|
| 靶标 | |
| 背景说明 |
类固醇激素及其受体参与调控真核生物基因表达，影响靶组织的细胞增殖和分化。根据异构体的不同，孕激素受体起转录激活或抑制的作用。
|
| 细胞定位 |
细胞核
|
| UniProt |
P06401
|
BioVendor热销产品——Soluble Receptor for Advanced Glycation End P
晚期糖基化终产物受体(RAGE)属于免疫球蛋白超家族，由319个氨基酸长的胞外区、疏水跨膜区和43个氨基酸组成，高度带电，胞质尾部。受体的胞外区由三部分组成:一个免疫球蛋白样V型结构域和两个免疫球蛋白样C型结构域。表明分子的氨基末端部分负责配体的结合。另一方面，羧基末端部分负责调节信号作用。
RAGE表达在几类细胞的整个发育过程中降低，包括神经元、内皮细胞、周细胞、平滑肌细胞、单核吞噬细胞、心肌细胞、肝细胞、肾小球上皮细胞或足细胞以及视网膜的穆勒和双极神经节细胞。有趣的是，成人组织中肺泡上皮细胞中RAGE的表达水平仍然很高。
RAGE与几种疾病和病理状态有关(例如糖尿病并发症、血管和炎症疾病)。其特征是晚期糖基化终产物(AGEs)受体，但该受体也能结合其他配体，如促炎性S100蛋白/钙粒蛋白、两性霉素或淀粉样β肽。AGEs是通过还原糖与蛋白质、脂类和核酸的游离氨基的非酶反应形成的物质组。
除了全长RAGE，还发现了这种受体的几种截短亚型。第一，编码分子的剪接变异体缺乏氮末端区域，第二，变异体缺乏碳末端。缺乏碳末端部分的RAGE剪接变异体缺少跨膜区，分泌到细胞外环境后，它作为内源性分泌RAGE (esRAGE)在血液中循环。esRAGE成功地与AGEs结合，由于这种能力，它可以通过阻止细胞表面全长RAGE的激活而作为拮抗剂。在血清中，可以检测到esRAGE以及另一种可能由基质金属蛋白酶蛋白水解切割产生的可溶性RAGE。sRAGE水平降低是人类炎症控制不足和/或改变的生物标志物。研究表明，sRAGE水平的降低与冠心病的高风险相关。与血管性痴呆患者和对照组相比，阿尔茨海默病患者的血清sRAGE降低。
产品详情：
检测极限： 19.2 pg/ml
检测范围：50–3200 pg/m
检测样本类型：Serum, Plasma
标准曲线：
订购详情：
|
产品名称 |
货号 |
规格 |
|
RD191116200R |
Soluble Receptor for Advanced Glycation End Products (sRAGE) Human ELISA |
96 wells (1 kit) |
更多详情请联系BioVendor中国代理商上海金畔生物
上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。
| 单嗜性受体增强剂Ecotropic Receptor Booster | ||||||
| 品牌 | Code No. | 产品名称 | 包装量 | 价格（元） | 说明书 | 数量 |
| Clontech | 631471 | Ecotropic Receptor Booster | 20 Rxns | ￥3,941 |  |
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产品详情请点击： |
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页面更新：2024-02-29 10:20:04
上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。
| 单嗜性受体增强剂Ecotropic Receptor Booster | ||||||
| 品牌 | Code No. | 产品名称 | 包装量 | 价格（元） | 说明书 | 数量 |
| Clontech | 631471 | Ecotropic Receptor Booster | 20 Rxns | ￥3,941 | |
|
| Ecotropic Receptor Booster用于临时提高您希望感染的任何靶细胞表面上的单嗜性受体蛋白质mCAT-1的密度。比如，可以使用单嗜性逆转录病毒或慢病毒有效转导人细胞，而原本它们只能感染啮齿类动物细胞。使用这项技术，还可以提高抵抗病毒感染的任何细胞类型的感染效率。 |
| 推荐使用以下产品来制备单嗜性假型病毒： |
| ·单嗜性慢病毒—Lenti-X Packaging Single Shots(Ecotropic) ·单嗜性逆转录病毒—Retro-X Universal Packaging System |
| 它的工作机制是什么呢？ |
| Ecotropic Receptor Booster由一小瓶浓缩的外泌体样囊泡组成，mCAT-1受体蛋白质密集包被在这些囊泡上。将囊泡增强剂作用于靶细胞（6孔板每孔10-20 μl，作用～2小时），它们会与靶细胞细胞膜相融合，从而使细胞表面受体水平升高。这样使用单嗜性假型慢病毒或逆转录病毒就可以更有效地转导靶细胞。 |
| ■ 概述/特点 |
| · 使用单嗜性假型慢病毒或逆转录病毒转导任何细胞类型，包括人细胞 · 高感染效率，不论要转导的是何种细胞类型 · 通过临时提高靶细胞病毒受体蛋白质的浓度来提高转导效率 · 病毒感染之前，只需将目标细胞与这些浓缩外泌体样囊泡预孵育2小时即可 · Ecotropic Receptor Booster可为靶细胞提供高密度mCAT-1受体 |
| ■ 实验例 |
 |
| 图1. Receptor boosters是在病毒感染前作用于靶细胞的浓缩外分泌体样囊泡。这种增强剂可提高转导效率，原因是经过处理后增加了细胞表面慢病毒（单嗜性）或逆转录病毒（单嗜性或兼嗜性）识别受体的密度。使用这项技术，比如，可以用单嗜性受体（否则就不存在）包被人细胞，使它们能够被单嗜性逆转录病毒或单嗜性慢病毒所转导。 |
 |
| 图2. 无论哪种细胞系类型，使用单嗜性慢病毒转导人细胞都能获得高感染效率。Panel A. 转导前24小时将HT1080细胞接种在6孔板中，添加10 μl Ecotropic Receptor Booster孵育2小时。然后使用Lenti-X HTX Ecotropic Packaging System (该产品已终卖，升级产品为Lenti-X Packaging Single Shots (Ecotropic) (Code No. 631278) )制备的Lenti-X ZsGreen1慢病毒（MOI=～15）转导HT1080细胞，48小时后分析ZsGreen1的表达情况。Panel B. 使用Ecotropic Receptor Booster进行预处理后，使通常抵抗单嗜性假型慢病毒载体转导的多种人细胞类型变得易于转导。 |
 |
| 图3. 通常情况下，使用单嗜性假型慢病毒无法感染Jurkat细胞、人间充质干细胞或人原代神经祖细胞。然而，这些细胞类型经过Ecotropic Receptor Booster预处理后都可进行非常有效的转导。 |
 |
| 图4. 631471: Ecotropic Receptor Booster |
| 产品详情请点击： |
页面更新：2024-02-29 10:19:24
Rabbit anti-Asialoglycoprotein Receptor 1 Recombinant Monoclonal Antibody(292-48)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
ASGP-R 1; ASGPR 1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1; ASGR1; CLEC4H1
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB: 1:1000-1:5000, IHC-P: 1:2000
|
| 免疫原 |
Recombinant protein
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
292-48
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Asialoglycoprotein receptors (ASGP-R; exist as subtypes 1 and 2) are located on the surface of hepatic cell membranes and are involved in the binding and endocytosis of glycoproteins that have exposed carbohydrate or N-acetylgalactosamine residues. In addition, the decreased level of ASGP-R on the hepatic cells has been shown to correlate clinically with the degree of liver function retained due to the development of cirrhosis, cancer, or viral hepatitis [PMID: 20641215].
|
| 细胞定位 |
Secreted, Membrane
|
| UniProt |
P07306
|
实验结果图
WB result of Asialoglycoprotein Receptor 1 Rabbit mAb Primary antibody: Asialoglycoprotein Receptor 1 Rabbit mAb at 1/5000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: HepG2 whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 33kDa Observed MW: 40~50kDa Exposure time: 5s
WB result of Asialoglycoprotein Receptor 1 Rabbit mAb Primary antibody: Asialoglycoprotein Receptor 1 Rabbit mAb at 1/5000 dilution Lane 1: mouse liver lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 33kDa Observed MW: 40kDa Exposure time: 30s
WB result of Asialoglycoprotein Receptor 1 Rabbit mAb Primary antibody: Asialoglycoprotein Receptor 1 Rabbit mAb at 1/1000 dilution Lane 1: rat liver lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 33kDa Observed MW: 40kDa Exposure time: 60s
IHC shows positive staining in paraffin-embedded human liver. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Rabbit anti-sFlt-1/VEGF Receptor 1 Recombinant Monoclonal Antibody(233-29)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
VEGFR-1, FRT
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
IHC-P: 1:1000
|
| 免疫原 |
Recombinant protein
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.25 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
233-29
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Vascular endothelial growth factor (VEGF) represents a growth factor with important pro-angiogenic activity, having a mitogenic and an anti-apoptotic effect on endothelial cells, increasing the vascular permeability, promoting cell migration, etc. VEGF binds to tyrosine kinase cell receptors (VEGFRs): VEGFR-1 [Fms-like tyrosine kinase 1 (Flt-1)], VEGFR-2 [kinase insert domain receptor (KDR) in human; fetal liver kinase 1 (Flk-1) in mouse] and VEGFR-3 [Fms-like tyrosine kinase 4 (Flt-4)]. While VEGFR-1 and VEGFR-2 are expressed predominantly on vascular endothelial cells, VEGFR-3 is expressed especially on lymphatic endothelial cells [PMID: 30173249].
|
| 细胞定位 |
Secreted
|
| UniProt |
P17948-2
|
实验结果图
IHC shows positive staining in paraffin-embedded human placenta. Anti-sFlt-1/VEGF Receptor 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-sFlt-1/VEGF Receptor 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-sFlt-1/VEGF Receptor 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-sFlt-1/VEGF Receptor 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
阿普洛尔盐酸盐
| EC | EINECS 237-244-4 |
| MDL | MFCD00069212 |
| 描述 | 是beta adrenergic receptor拮抗剂，也是5-HT1A受体的拮抗剂。 |
| 别名 | 烯丙心胺;盐酸阿普洛尔 |
| 英文名称 | Alprenolol Hydrochloride |
| CAS | 13707-88-5 |
| 分子式 | C15H24ClNO2 |
| 分子量 | 285.81 |
| 纯度 | ≥98% |
| 单位 | 瓶 |
| SMILES | OC(COC1=CC=CC=C1CC=C)CNC(C)C.[H]Cl |
| 靶点 | Adrenergic Receptor; 5-HT Receptor |
| 规格 | 10mg 50mg |
清道夫受体B2抗体（Scavenger Receptor B2/CD36 Monoclonal Antibody） Item No. 10009893
本文是对Cayman热销产品清道夫受体B2抗体（Scavenger Receptor B2/CD36 Monoclonal Antibody）的产品简单介绍，该产品货号为10009893，如需购买或咨询该产品其它详情，请联系Cayman代理商上海金畔生物。
别名：GPIIIb
GPIV
Platelet Glycoprotein 4
Fatty Acid Translocase
PAS IV
Platelet Collagen Receptor
Thrombospondin Receptor
免疫原：Recombinant full-length mouse CD36 expressed in adenovirus
克隆号：JC63.1
缓冲液：PBS, pH 7.2
宿主：CD36 null mouse
亚型：IgA
应用：FC, ICC
产品介绍
CD36也被称为清道夫受体B2或脂肪酸转位酶，是一种跨膜糖蛋白，在脂肪酸摄取、脂质代谢和信号转导中发挥作用。它由N和C端胞质尾区，两个跨膜域和一个细胞外循环，与多种脂质结合，包括长链脂肪酸，氧化低密度脂蛋白和氧化磷脂和蛋白质配体。多种细胞可表达CD36，包括血小板、单核细胞、巨噬细胞等造血细胞，以及脂肪细胞、肠细胞、心肌和骨骼肌细胞、内皮细胞和上皮细胞。
CD36的表达受多种转录因子调控，包括过氧化物酶体增殖物激活受体(PPAR)、STAT3和HIF-1a，并以一种组织依赖的方式进行调控。Cayman的Scavenger Receptor B2/CD36单克隆抗体(Clone JC63.1)内毒素水平≤2 EU/mg可用于流式细胞术(FC)和免疫细胞(ICC)应用。
详细使用说明请见https://cdn.caymanchem.com/cdn/insert/10009893.pdf
产品信息
|
货号 |
名称 |
规格 |
|
10009893 |
Scavenger Receptor B2/CD36 Monoclonal Antibody (Clone JC63.1) (Low Endotoxin) |
100μg / 500μg |
详情请咨询 Cayman 中国代理-上海金畔生物科技
阿拉莫林
| 有效期 | 2年 |
| 描述 | 是一种新型 ghrelin receptor 激动剂。 |
| 别名 | RC-1291 |
| 英文名称 | Anamorelin |
| CAS | 249921-19-5 |
| 分子式 | C31H42N6O3 |
| 分子量 | 546.70 |
| 储存条件 | -20℃ |
| 纯度 | ≥98% |
| 外观（性状） | White to off-white Solid |
| 单位 | 瓶 |
| 生物活性 | Anamorelin (RC-1291) is a potent ghrelin receptor agonist with EC50 value of 0.74 nM in the FLIPR assay.[1] |
| IC50 | Ki: 0.7 nM (ghrelin receptor)[1] |
| In Vitro | In the FLIPR assay, Anamorelin (ANAM) shows significant agonist activity on the ghrelin receptor, with EC50 value of 0.74 nM. No significant antagonist activity is observed with Anamorelin at concentrations of up to 1,000 nM. In the binding experiments, Anamorelin binds to the ghrelin receptor with a binding affinity constant (Ki) of 0.70 nM. In the competition assay with radiolabeled ibutamoren (35S-MK-677; another ghrelin receptor agonist) Anamorelin (ANAM) is also found to bind with high affinity to the ghrelin receptor (IC50=0.69 nM). In rat pituitary cells incubated with Anamorelin, there is a dose-dependent stimulatory effect on GH release and the potency (EC50) is 1.5 nM. Anamorelin is screened for activity against a set of over 100 receptors, ion channels, transporters, and enzymes. Anamorelin demonstrates binding to the tachykinin neurokinin 2 (NK2) site (IC50=0.021 μM); however, a subsequent NK2 functional assay demonstrates no functional activity[1]. |
| In Vivo | In rats, Anamorelin (ANAM) at an oral dose of 3, 10, or 30 mg/kg once daily significantly increases both food intake and body weight from Day 2 to Day 7 of treatment compared with the vehicle control. The cumulative change in food intake and weight gain increases dose-dependently, and these changes are significant at all dose levels (P<0.05) compared to the control. Administration of Anamorelin at a single oral dose of 3, 10, or 30 mg/kg induces a dose-dependent increase in plasma GH levels and GH AUC0-6h in rats[1]. |
| 激酶实验 | For the competition assay, Anamorelin (ANAM) concentrations (1 pM-10 μM) are added to the membranes together with 35S-MK-677. Nonspecific binding is determined by adding 10 μM nonlabeled MK-677. The mixture is incubated at 30°C for 60 min, followed by application of the samples to GF/B filters, which has been pretreated with 0.5 % PEI for 60 min. The filters are subsequently washed in 0.9 % NaCl and counted using an OptiPhase counter[1]. |
| SMILES | [H][C@](CC1=CNC2=CC=CC=C21)(NC(C(C)(C)N)=O)C(N3CCC[C@@](C(N(C)N(C)C)=O)(CC4=CC=CC=C4)C3)=O |
| 靶点 | ghrelin receptor |
| 细胞实验 | Rats[1]For the assessment of food intake and body weight, rats are divided into four groups: Anamorelin 3 mg/kg (n=7), 10 mg/kg (n=7), or 30 mg/kg (n=7), or vehicle control (n=8), and 100 μL blood samples are collected before and 0.25, 0.5, 1, 2, 3, 4, 5, and 6 h after single dosing. Rats are anesthetized with sodium pentobarbital 64.8 mg/kg. A catheter filled with heparinized saline solution is inserted in the left femoral artery for blood collection and fitted with an extension tube, 1 mL sampling syringe, and a three-way cock to allow excess blood to return. Plasma levels of GH are measured immunochemically using a Rat Growth Hormone EIA kit and microplate reader. Measurements are performed in duplicate. Area under the GH concentration curve from 0 to 6 h (AUC0-6h) postdose and the time course of GH plasma concentrations are evaluated. Pig[1]In pigs (n=6 per group), Anamorelin is dosed directly into the gastric lumen via the dosing catheter. Blood samples are collected for the stimulation profile of GH at 30 and 15 min before, and 0, 5, 15, 30, 45, 60, and 120 min following dosing. Animals received either a single dose (3.5 mg/kg), or once-daily administration (1 mg/kg) for 7 days and stimulation profiles are taken after the first and seventh dose of Anamorelin. To assess IGF-1 levels, pigs receive either placebo or Anamorelin for 7 days (1 mg/kg/day), and the following 7 days the two treatments are crossed over. A single blood sample is taken once a day immediately before dosing. |
| 数据来源文献 | [1]. Pietra C, et al. Anamorelin HCl (ONO-7643), a novel ghrelin receptor agonist, for the treatment of cancer anorexia-cachexiasyndrome: preclinical profile. J Cachexia Sarcopenia Muscle. 2014 Dec;5(4):329-37. |
| 规格 | 5mg 10mg |
盐酸罗替戈汀
| 有效期 | 2年 |
| 描述 | 是 Dopamine receptor 的激动剂，也是 5-HT1A receptor 的部分激动剂，以及 α2B-adrenergic receptor 的拮抗剂。 |
| EC | EINECS 603-080-0 |
| 别名 | 罗替戈汀盐酸盐, N-0923 Hydrochloride |
| 英文名称 | Rotigotine Hydrochloride |
| CAS | 125572-93-2 |
| 分子式 | C19H26ClNOS |
| 分子量 | 351.93 |
| 储存条件 | -20℃ |
| 纯度 | ≥98% |
| 外观（性状） | White to off-white Solid |
| 单位 | 瓶 |
| SMILES | OC1=C2CC[C@@H](CC2=CC=C1)N(CCC3=CC=CS3)CCC.[H]Cl |
| 靶点 | Dopamine Receptor |
| 规格 | 5mg 10mg 25mg |