Rabbit anti-CD155 Recombinant Monoclonal Antibody(289-68)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
PVR; Poliovirus receptor; Nectin-like protein 5; NECL-5; PVS
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:1000, IHC-P: 1:1000-1:2000
|
| 免疫原 |
Recombinant protein
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.25 mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
289-68
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Poliovirus receptor (PVR, CD155), a member of the nectin-like family of proteins, is recently emerging as a promising target in immunotherapy that enhances the existing anti-tumor responses. Similar to other members of the family, PVR is involved in important cellular processes, such as adhesion, contact inhibition, migration, proliferation, and the immune response [PMID: 18648374]. PVR is dramatically overexpressed in several human malignancies, whereas its expression is low or JPent in most healthy tissues [PMID: 10841575, PMID: 11454801, PMID: 15604290, PMID: 15536144, PMID: 19801517]. Consistent with PVR biology, its overexpression promotes tumor cell invasion, migration, and proliferation and is associated with a poor prognosis and enhanced tumor progression [PMID: 16322240, PMID: 17893876, PMID: 25743219, PMID: 25862891]. Simultaneously, PVR has a considerable immu noregulatory potential. It interacts with the (co)stimulatory receptor DNAM-1 and the inhibitory receptors TIGIT and CD96, resulting in either immune cell activation or inhibition, respec tively [PMID: 28870470].
|
| 细胞定位 |
Cell membrane, Secreted
|
| UniProt |
P15151
|
实验结果图
WB result of CD155 Rabbit mAb Primary antibody: CD155 Rabbit mAb at 1/1000 dilution Lane 1: Raji whole cell lysate 20ug Lane 2: HUVEC whole cell lysate 20ug Lane 3: HeLa whole cell lysate 20ug Negative control: Raji whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 45kDa Observed MW: 70kDa Exposure time: 90s
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD155/PVR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-CD155/PVR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CD155/PVR antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CD155/PVR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.