Rabbit anti-PI3K p85 α(pY467/199) Polyclonal Antibody描述别名宿主特异性反应种属应用分子量免疫原纯化方法类型储存/保存方法UniProt
| 概述 | |
| 描述 |
Rabbit polyclonal antibody to PI3K p85 alpha/p55 gamma (pY467/199)
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| 别名 |
PI3K p85 alpha抗体, p55 gamma抗体;PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R3; Phosphatidylinositol 3-kinase regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK
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| 宿主 |
Rabbit
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| 特异性 |
Recognizes endogenous levels of PI3K p85 alpha/p55 gamma (pY467/199) protein.
|
| 反应种属 |
Human, Mouse, Rat, Bovine
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| 应用 |
WB 1:500 – 1:1000 , IHC 1:50 – 1:200
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| 分子量 |
Detects a band of approximately 85kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PI3K p85 alpha/p55 gamma (pY467/199). The exact sequence is proprietary.
|
| 性能 | |
| 纯化方法 |
The antibody was purified by immunogen affinity chromatography.
|
| 类型 |
Polyclonal Antibody
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| 储存/保存方法 |
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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| 靶标 | |
| UniProt |
P27986
|
实验结果图
Western blot analysis of PI3K p85 alpha/p55 gamma (pY467/199) expression in HEK293T (A), NIH3T3 (B) whole cell lysates.
Immunohistochemical analysis of PI3K p85 alpha/p55 gamma (pY467/199) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.