标签归档：purification

Purification ReagentWHATMAN FTA纯化试剂WB120204

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【简单介绍】

WHATMAN FTA纯化试剂WB120204 Purification Reagent，500ml。Our Whatman FTA Purification Reagent is used for purifying nucleic acids stored on FTA Cards.

【详细说明】

WHATMAN FTA纯化试剂WB120204

FTA Purification Reagent 500 ml

Our Whatman FTA Purification Reagent is used for purifying nucleic acids stored on FTA Cards.

  • For use with Whatman FTA Cards, Indicating FTA Cards, and EasiCollect device for the purification of nucleic acids.
  • Proprietary chemistry ensures high-quality DNA for STR analysis.
  • 500 ml bottle for an increased number of reactions.

FTA Purification Reagent is used to purify DNA captured and stored on FTA Cards. This reagent can be used to wash punches in the “punch-in” procedure for standard STR amplification.

FTA Purification Reagent allows for excellent results by removing PCR inhibitors and contaminations, which can lower PCR yields. High-quality DNA can be achieved from samples washed with the FTA Purification Reagent due to the propriety chemistry that facilitates DNA release from the FTA matrix. The versatility of the FTA Purification Reagent allows the user to purify DNA captured on FTA Cards, Indicating FTA Cards, and EasiCollect devices.

WHATMAN FTA纯化试剂WB120204订购信息：

WB120028 IND CLONESAVER 5/PK
WB120052 CLONESAVER STARTER 1/PK
WB120055 FTA MINICARD 100/PK
WB120056 IND FTA MINICARD 100/PK
WB120065 FTA PLANTSAVER 100/PK
WB120067 FTA KIT 1/PK
WB120068 FTA PLANT KIT 1/PK
WB120205 FTA CLASSIC 100/PK
WB120206 IND FTA CLASSIC 100/PK
WB120208 FTA GENECARD/100/PK
WB120210 FTA MICROCARD 100/PK
WB120211 IND FTA MICROCARD 100/PK
WB120305 FTA CLASSIC 25/PK
WB120306 IND FTA CLASSIC 25/PK
WB120308 FTA GENECARD 25/PK
WB120310 FTA MICROCARD 25/PK
WB120311 IND FTA MICROCARD 25/PK
WB120312 FTA CLASSIC 250/PK
WB120313 FTA MINICARD 250/PK
WB120315 IND FTA CLASSIC 250/PK
WB120316 FTA MICROCARD 250/PK
WB120317 IND FTA MINICARD 250/PK
WB120318 IND FTA MICROCARD 250/PK
WB120355 FTA MINICARD 25/PK
WB120356 IND FTA MINICARD 25/PK
WB120365 FTA PLANT SAVER 25/PK
WB120462 EASICOLLECT DEVICE 50/PK
WB120319 FTA PLANTSAVER CARD 250PK
WB120314 FTA GENECARD CASE OF 250
WB120237 EASICOLLECT KIT 50/PK
WB120239 FTA BUCCAL COLLECTION KIT 50PK
WB120238 FTA BLOOD COLLECTION KIT 50/PK
WB100003 DESICCANT 1000/PK
WB100005 MICROPUNCH 1.2MM 1/PK
WB100006 MICROPUNCH TIP 1.2MM 1/PK
WB100007 MICROPUNCH 2.0MM 1/PK
WB100008 MICROPUNCH TIP 2.0MM 1/PK
WB100014 BLOODSTAIN CARD 100/PK
WB100020 CUTTING MAT 1/PK
WB100024 CLONESAVER RESEAL POUCH 50/PK
WB100025 MICROPUNCH PLUNGER 1.2MM 1/PK
WB100026 MICROPUNCH PLUNGER 2.0MM 1/PK
WB100028 UNICORE PUNCH 1.2MM 4/PK
WB100029 UNICORE PUNCH 2.0MM 4/PK
WB100030 FTA GENECARD TRAY 20/PK
WB100032 FOAM APPLICATOR 100/PK
WB100034 SPOT HOLDER 1/PK
WB100035 OMNISWAB 100/PK
WB100036 POUCH 3.75INX3IN 100/PK
WB100037 POUCH 4.37INX6.5IN 100/PK
WB100038 MICROPUNCH 3.0MM 1/PK
WB100039 UNICORE PUNCH 3.0MM 4/PK
WB100040 UNICORE PUNCH 6.0MM 4/PK
WB100041 MICROPUNCH PLUNGER 3.0MM 1/PK
WB100042 MICROPUNCH TIP 3.0MM 1/PK
WB120112 FTA REAGENT 25ML 1/PK
WB120204 FTA REAGENT 500ML 1/PK
WB300028 UNICORE PUNCH 1.20MM 4/PK
WB300204 FTA REAGENT 500ML 1/PK
WB100048 HARRIS E-CORE 2.0MM
WB100052 HARRIS E-CORE 1.2mm
28977442 FTA Reagent 1 L
WB100059 HARRIS MICROPUNCH 0.50MM PLAST
WB100060 HARRIS MICROPUNCH 1.00MM PLAST
WB100061 HARRIS MICROPUNCH 1.20MM PLAST
WB100062 HARRIS MICROPUNCH 2.00MM PLAST
WB100063 HARRIS MICROPUNCH 3.00MM PLAST
WB100064 HARRIS MICROPUNCH 0.50MM ALUM
WB100065 HARRIS MICROPUNCH 1.00MM ALUM
WB100066 MICROPUNCH TIP 0.50MM
WB100067 HARRIS MICROPUNCH TIP 1.00MM
WB100068 HARRIS PLUNGER FOR 0.50MM TIP
WB100069 HARRIS PLUNGER FOR 1.00MM TIP
WB100070 HARRIS 25XUNI-CORE 0.35MM
WB100071 HARRIS 25XUNI-CORE 0.50MM
WB100072 25X UNI-CORE 0.75MM
WB100073 25X UNI-CORE 1.00MM
WB100074 25XUNI-CORE 1.20MM
WB100075 25X UNI-CORE 1.50MM
WB100076 25X UNI-CORE 2.00MM
WB100077 HARRIS 25XUNI-CORE 2.50MM
WB100078 HARRIS 25XUNI-CORE 3.00MM
WB100079 HARRIS 25XUNI-CORE 3.50MM
WB100080 HARRIS 25XUNI-CORE 4.00MM
WB100081 25XUNI-CORE 5.00MM
WB100082 HARRIS 25XUNI-CORE 6.00MM
WB100083 25XUNI-CORE 7.00MM
WB100084 25XUNI-CORE 8.00MM
WB100085 HARRIS 25XUNI-CORE 10.00MM
WB100086 HARRIS 25XUNI-CORE 12.00MM
WB100088 CUTTING MAT 2 X 3 IN 1/PK
80-5123-17 foam swab
WB100004 STERILE OMNI SWAB 400/PK
WB120203 FTA A4 SHEETS F572-2 100/PK
WB129111 NY FTA MICROCARD 100/PK
WB129125PN POLICE NATIONALE FTA KIT 1/PK
WB129136 ISRAEL FTA CARD 100/PK
WB129139 RCMP FTA CARD 100/PK
WB129142 SOUTH DAKOTA FTA CARD 100/PK
WB129147 ME/VT FTA CARD 100/PK
WB129151 CONNECTICUT FTA KIT 1/PK
WB129152 GENVAULT F572-02 120MM
WB129153 HALLY LABELS F572-02 13MM
WB129163 FBI FTA CARD FITZCO 100/PK
WB129164 CIFS FTA KIT 1/PK
WB129173 IMESC FTA CARD 100/PK
WB129174 MISSOURI FTA CARD 100/PK
WB129180 SPANISH ARMY FTA KIT 1/PK
WB129181 TIANJIM SECURITY FTA 100/PK
WB129183 TAIWAN CIB FTA KIT 1/PK
WB129184 DENMARK CARD 100/PK
WB129185 MICHIGAN ST FTA KIT 1/PK
WB129191 ISRAEL FTA CARD 100/PK
WB129193 MERIAL FTA CARD PK 100
WB129203 LUXEMBOURG FTA KIT 1/PK
WB129206 FTA CARD 100/PK
WB129211 SHANGHAI MP FTA CARD 100/PK
WB129214 HAWAII FTA KIT 1/PK
WB129219 JAPAN OMNISWAB KIT 1/PK
WB300032 FOAM APPLICATOR 100/PK
WB300055 FTA MINI CARD 100/PK
WB300205 FTA CLASSIC CARD 100/PK
WB300210 FTA MICRO CARD 100/PK
WB129228 MARYLAND FTA ELUTE KIT
WB129232 FTA NORWAY POLICE 1/PK
WB129233 FBI BUCCAL KIT 1/PK
WB129234 FTA KIMIA CARD 100/PK
WB129236 CUSTOM FTA CARD 100/PK
WB129237 CUSTOM FTA CARD 100/PK
WB129235 GUARDIA CIVIL FTA KIT
WB129238 SINGAPORE WITNESS KIT 1
WB129240 SINGAPORE SUSPECT KIT 1
WB129239 SINGAPORE OFFENDER KIT 1
WB129227 WASTATE POLICE OFFND 100/PK
WB129212 TEXAS FTA SHEETS 100/PK
WB129140 NJ STATEPOLICE FTA CRD 100/PK
WB100051 FBI DATA FORM 5/PK
WB100054 DESICCANT (2/POUCH) 100PK
WB129244 FTA KIT BRUNEI 1/PK
WB129245 ANGUS FTA CARD 100/PK
WB129246 FTA TAIWAN CIB 100/PK
WB100056 FBI CLEAR POUCH 100PK
WB129249 FRENCH POLICE FTA KIT 1/PK
WB129252 FTA VERMONT 100PK
WB129257 ITALIAN HAIRCARD PROTOCOL
WB129263 ALABAMA BUCCAL KIT B
WB129264 ALABAMA BUCCAL KIT A
WB129261 FTA BUCCAL KIT FOR INDIA
WB129262 FTA BLOOD KIT FOR INDIA
WB129268 MICHIGAN FTA CARD 100/PK
WB129271 MAINE FTA CARD 100/PK
WB129270 CUSTOM EASICOLLECT CARD 100/PK
WB129266 ICMP DNA PROGRAM KIT, 1/PK
WB100057 STERILE 6IN COTTON SWAB PLASTIC SHAFT
WB129233AA FBI BUCCAL KIT 1/PK REPACK
WB129273 NEBRASKA EASICOLLECT KIT 1/PK
WB129258 MALAYSIA FTA KIT 1/PK
WB129182 FINGERPRINT AMERICA FTA 100PK
WB100087 STERILE OMNISWAB 1000/PK
WB129277 CALIFORNIA FTA CARD 100/PK
WB129282 NEVADA EASICOLLECT KIT 1/PK
WB129269 FBI BUCCAL KIT No2 1/PK
WB129281 NEXT OF KIN IDENTIFICATION SINGAPORE FTA KIT 1/PK
WB129285 IOWA CUSTOM FTA KIT 1/PK
WB120226 Magazine for Large Format Plastic Cassettes
WB120227 Magazine for Large Format Cards
WB120228 Magazine for Plastic Format Easicollect Cards
WB120229 Magazine for Paper format Easicollect
WB129290 PHILIPPINES CUSTOM FTA CARD 100/PK
WB650075 LAS VEGAS CUSTOM EASICOLLECT 50/PK
WB129297 CHINA EASICOLLECT CARD 100/PK
WB120088 FTA MINICARD (CUSTOM) 100/PK
WB100089 CLEAR POUCH 100/PK
WB129301 FBI FTA BUCCAL KIT 1/PK

质粒提取试剂盒TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0酶试剂盒Takara Clontech

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上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。

质粒提取试剂盒TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Takara 9760 TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 50 Rxns ￥381 质粒提取试剂盒TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 质粒提取试剂盒TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 质粒提取试剂盒TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0
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■ 制品内容 (50 次量)
本试剂盒分试剂Set 与Column Set两部分。
□ 试剂Set  
RNase A (10 mg/ml) 140 μl
Solution Ⅰ 14 ml
Solution Ⅱ*1 14 ml
Solution Ⅲ*2 24 ml
Buffer WA*2 28 ml
Buffer WB*3 24 ml
Elution Buffer 2 ml × 2
*1含有强碱溶液，应避免与皮肤、衣物等接触。若不小心接触到眼睛或皮肤时，请立即到医院进行处理。
*2 含有强变性剂，应避免与皮肤、衣物等接触。若不小心接触到眼睛或皮肤时，请立即到医院进行处理。
*3 首次使用前，向Buffer WB中添加56 ml的100%乙醇。
 
□ Column Set  
Spin Columns 50 支
Collection tubes 50 支
 
■ 制品说明
本试剂盒是用于质粒 (Plasmid) DNA小量纯化的试剂盒。试剂盒采用了传统的SDS碱裂解法，结合硅胶膜技术，具有高效、快速、方便之特点，全套操作只需1小时便可完成。使用本试剂盒可从1～4 ml LB培养基过夜培养的菌液中纯化得到1～20 μg的高纯度质粒DNA (OD260/OD280 = 1.8～2.0)，制备过程无需苯酚抽提、乙醇沉淀等步骤，所得质粒DNA溶解于Tris Buffer或水中，纯度较高，可直接用于转化、DNA序列分析、体外转录、限制酶切以及其他各种酶促反应。
 
■ 保存与运输
1. 本试剂盒可以在室温下（15-25℃）保存，但温度较低时，有的Buffer会出现沉淀，使用前需37℃加热直至沉淀消失。
2. SolutionⅠ中加入RNase A后可于4℃保存约3个月。
3. RNase A可于室温下 (15-25℃) 保存6个月，长期保存需存放于-20℃。
4. 本试剂盒于室温下 (15-25℃) 运输。
 
■ 实验操作流程图
质粒提取试剂盒TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0
 
■ 注意事项
1. 每次起始的菌液量应控制在1～4 ml，菌量太大影响溶菌及质粒DNA的释放，纯化时会影响质粒DNA的纯度。菌体的培养时间不要超过16小时，否则难以裂解。
2. 加入Solution Ⅱ和Solution Ⅲ后，不要剧烈混合（Vortex等），剧烈混合会导致基因组DNA的污染。
3. 加入Solution Ⅲ后，应充分混合使蛋白质、基因组DNA等形成白色沉淀，离心后沉降于离心管底部。若离心后沉淀仍悬浮于溶液中时，请将离心管上下翻转混合数次后高速离心3～5分钟。
4. 纯化的质粒DNA用于DNA序列分析时，最好使用灭菌蒸馏水洗脱质粒DNA。
5. 质粒DNA需长期保存时，建议在Elution Buffer中保存。
 
 

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