21435-PIERCE,EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit-琼脂糖

【简单介绍】

货号:21435,名称:EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit,品牌:PIERCE,规格:KIT

【详细说明】

订货:

 

EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

产品特点:

 

提供的试剂足以完成10次标记反应(货号:21455的试剂盒可以完成8次反应)。

每次反应可以标记1-10毫克蛋白,反应体系为0.5-2毫升。

• NHS-酯生物素专一地与伯胺反应(N-末端及赖氨酸残基的侧链)形成稳定的酰胺键。

• NHS酯的磺酸盐形式(或者是货号:21955的试剂盒中的PEG 间隔臂)促进生物素试剂的水溶性。

使用Zeba脱盐离心柱(5毫升)可以进行快速的样品净化和高效的蛋白回收速度比透析及传统的凝胶过滤更快,蛋白回收率和脱盐效率比超滤的更好

提供测定标记效率的试剂和方法。

 

EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit现货供应

 

PIERCE

21425

EZ-Link (R) Sulfo-NHS Biotinylation Kit

KIT

PIERCE

21435

EZ-Link (R) Sulfo-NHS-LC      Biotinylation Kit

KIT

PIERCE

21441

EZ-Link (R) NHS-SS-Biotin,    50 mg.

50 MG

 

EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit相关产品

 

产品货号

产品名称

包装

21217

Sulfo-NHS-Biotin§

也提供非磺化的透膜的类似物

50毫克

21326

Sulfo-NHS-Biotin,无需称量。

8 x 2毫克

21425

Sulfo-NHS-Biotinylation试剂盒

试剂盒

21925

微量Sulfo-NHS-Biotinylation试剂盒

试剂盒

 

EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit

仅用于科研

 

21343-PIERCE,EZ-Link (tm) Biotin-HPDP-琼脂糖

【简单介绍】

货号:21343,名称:EZ-Link ™ NHS-LC-LC-Biotin,品牌:PIERCE,规格:50 MG

【详细说明】

订货:

 

EZ-Link (tm) NHS-LC-LC-Biotin介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) NHS-LC-LC-Biotin现货供应

 

PIERCE

21343

EZ-Link (tm) NHS-LC-LC-Biotin

50 MG

PIERCE

21360

EZ-Link (R) Biotin-PEG4-Hydrazide, 50 mg.

50 MG

PIERCE

21425

EZ-Link (R) Sulfo-NHS Biotinylation Kit

KIT

 

EZ-Link (tm) NHS-LC-LC-Biotin品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (tm) NHS-LC-LC-Biotin仅用于科研

 

Thermo Scientific EZ-Link NHS-LC-LC-Biotin is a doubly long-chain, NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for intracellular labeling.

 

This is one of three EZ-Link NHS-Biotin Reagents that enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution. Differing only in their spacer arm lengths, the three reagents offer researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.

 

Highlights:

  • Lysine-reactive – label any antibody or other protein with biotin for use in protein methods
  • Irreversible – forms stable amide bonds that are permanent
  • Solubility – must be dissolved in DMSO or DMF before further dilution in aqueous buffers
  • Long reach – spacer arm (total length added to target) is 30.5 angstroms

     

     

  • Membrane-permeable – can be used to label inside cells (intracellular)

     

21341-PIERCE,EZ-Link (tm) Biotin-HPDP-琼脂糖

【简单介绍】

货号:21341,名称:EZ-Link ™ Biotin-HPDP,品牌:PIERCE,规格:50 MG

【详细说明】

订货:

 

EZ-Link (tm) Biotin-HPDP介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) Biotin-HPDP现货供应

 

PIERCE

21341

EZ-Link (tm) Biotin-HPDP,     50 Mg.

50 MG

PIERCE

21343

EZ-Link (tm) NHS-LC-LC-Biotin

50 MG

PIERCE

21360

EZ-Link (R) Biotin-PEG4-Hydrazide, 50 mg.

50 MG

 

EZ-Link (tm) Biotin-HPDP品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (tm) Biotin-HPDP

仅用于科研

 

Thermo Scientific EZ-Link HPDP-Biotin is pyridyldithiol-activated, sulfhydryl-reactive biotinylation reagent that conjugates via a cleavable (reversible) disulfide bond to enable use in a variety of purification methods.

 

Highlights:

  • Pyridyldithiol reacts with -SH groups to form a stable disulfide bond
  • Pyridine-2-thione leaving group can be used to measure degree of biotinylation at 343nm
  • Disulfide bond is cleavable using 50mM DTT, or 100mM beta-mercaptoethanol
  • Extender spacer arm prevents steric hindrance
  • Must be dissolved in DMSO or DMF before use
  • Biotinylation occurs between pH 6 to 9

  •  

21336-PIERCE,EZ-Link (tm) NHS-LC-Biotin-琼脂糖

【简单介绍】

货号:21336,名称:EZ-Link ™ NHS-LC-Biotin,品牌:PIERCE,规格:50 MG

【详细说明】

订货:

 

EZ-Link (tm) NHS-LC-Biotin介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) NHS-LC-Biotin现货供应

 

PIERCE

21336

EZ-Link (tm) NHS-LC-Biotin

50 MG

PIERCE

21338

EZ-Link (tm) Sulfo-NHS-LC-LC-Biotin

50 MG

PIERCE

21340

EZ-Link (tm) Biotin-LC-Hydrazide, 50 Mg.

50 MG

 

EZ-Link (tm) NHS-LC-Biotin品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

 

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (tm) NHS-LC-Biotin仅用于科研

 

Thermo Scientific EZ-Link NHS-LC-Biotin is a long-chain, NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for general intracellular labeling.

 

This is one of three EZ-Link NHS-Biotin Reagents that enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution. Differing only in their spacer arm lengths, the three reagents offer researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.

 

Highlights:

  • Lysine-reactive – label any antibody or other protein with biotin for use in protein methods
  • Irreversible – forms stable amide bonds that are permanent
  • Solubility – must be dissolved in DMSO or DMF before further dilution in aqueous buffers
  • Medium length – spacer arm (total length added to target) is 22.4 angstroms
  • Membrane-permeable – can be used to label inside cells (intracellular)

  •  

21335-PIERCE,EZ-Link (tm) Sulfo-NHS-LC-Biotin-琼脂糖

【简单介绍】

货号:21335,名称:EZ-Link ™ Sulfo-NHS-LC-Biotin, 100 mg,品牌:PIERCE,规格:100 mg

【详细说明】

订货:

 

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg现货供应

 

PIERCE

21335

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg

100 MG

PIERCE

21336

EZ-Link (tm) NHS-LC-Biotin

50 MG

PIERCE

21338

EZ-Link (tm) Sulfo-NHS-LC-LC-Biotin

50 MG

 

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

 

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg

仅用于科研

 

Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

 

EZ-Link Sulfo-NHS-Biotin Reagents enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.

 

Highlights:

  • Label almost any antibody with biotin to facilitate immobilization, purification or detection
  • Intermediate spacer arm (22.4 angstroms) provides excellent balance of adequate length (minimal steric hindrance for biotin binding) and modest mass (339.5 daltons)
  • Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
  • Sulfonate group attached to the biotinylation reagents increases reagent water solubility compared to ordinary NHS esters
  • Sulfo-NHS-LC-Biotin is water soluble, so biotin labeling can be done under physiologic conditions
  • High purity, crystalline biotin labeling reagents can be used to create high-quality biotin-labeled derivatives

  •  

企业内控号LTS1077006-人外周血淋巴细胞分离液(用于生物治疗细胞的分离) 内毒素<0.06EU 医用级-细胞分离液

【简单介绍】

货号:HY2015(企业内控号:LTS1077006) ,产品名称:样本密度分离液(人外周血淋巴细胞分离液)(医用级分离液,用于生物治疗细胞的分离) 内毒素<0.06EU 医用级 ,品牌:TBD,规格:200ml/瓶

【详细说明】

货号:HY2015(企业内控号:LTS1077006) ,产品名称:样本密度分离液(人外周血淋巴细胞分离液)(医用级分离液,用于生物治疗细胞的分离) 内毒素<0.06EU 医用级 ,品牌:TBD,规格:200ml/瓶

  

21331-EZ-Link (tm) Sulfo-NHS-SS-Biotin, 100 mg-琼脂糖

【简单介绍】

货号:21331,名称:EZ-Link ™ Sulfo-NHS-SS-Biotin, 100 mg,品牌:PIERCE,规格:100 mg

【详细说明】

订货:

 

EZ-Link (tm) Sulfo-NHS-SS-Biotin, 100 mg介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) Sulfo-NHS-SS-Biotin, 100 mg现货供应

 

PIERCE

21331

100 MG

PIERCE

21335

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg

100 MG

PIERCE

21336

EZ-Link (tm) NHS-LC-Biotin

50 MG

 

品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

 

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

仅用于科研

 

Thermo Scientific EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

Sulfo-NHS-SS-Biotin is a thiol-cleavable amine-reactive biotinylation reagent that contains an extended spacer arm to reduce steric hindrances associated with avidin binding. This reagent is water-soluble, enabling biotinylation to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents or are complicated by their inclusion.

Highlights:

  • Label almost any protein with biotin to facilitate immobilization, purification or detection
  • Ideal for reversible biotinylation; disulfide bond in the spacer arm allows the biotin label to be removed as desired
  • Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
  • Sulfonate group attached to the biotinylation reagent increases water solubility to a minimum of 6 mg/ml, so biotin labeling can be done under physiologic conditions
  • Long spacer arm on the biotin reduces steric hindrance when binding to avidin molecules

21327-EZ-Link(R) Sulfo-NHS-LC-Biotin, No-Weigh(tm) Forma-Pierce试剂盒

【简单介绍】

货号:21327,名称:EZ-Link(R) Sulfo-NHS-LC-Biotin, No-Weigh(tm) Format,品牌:PIERCE,规格:EA (8X1 MG)

【详细说明】

订货:

 

EZ-Link(R) Sulfo-NHS-LC-Biotin, No-Weigh(tm) Format介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link(R) Sulfo-NHS-LC-Biotin, No-Weigh(tm) Format现货供应

 

PIERCE

21327

t,

EA (8X1 MG)

PIERCE

21329

EZ-Link(R) NHS-PEG4-Biotin,   No-Weigh(tm) Format,

8 X 2 MG

PIERCE

21331

EZ-Link (tm) Sulfo-NHS-SS-Biotin, 100 mg

100 MG

 

t品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

t

仅用于科研

 

Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

 

EZ-Link Sulfo-NHS-Biotin Reagents enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.

 

Highlights:

  • Label almost any antibody with biotin to facilitate immobilization, purification or detection
  • Intermediate spacer arm (22.4 angstroms) provides excellent balance of adequate length (minimal steric hindrance for biotin binding) and modest mass (339.5 daltons)
  • Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
  • Sulfonate group attached to the biotinylation reagents increases reagent water solubility compared to ordinary NHS esters
  • Sulfo-NHS-LC-Biotin is water soluble, so biotin labeling can be done under physiologic conditions
  • High purity, crystalline biotin labeling reagents can be used to create high-quality biotin-labeled derivatives

21324-IMMUNOPURE STREPTAVIDIN, ALKALINE PHOSPHATASE CONJ-Pierce试剂盒

【简单介绍】

货号:21324,名称:IMMUNOPURE STREPTAVIDIN, ALKALINE PHOSPHATASE CONJUGATED,品牌:PIERCE,规格:1 MG

【详细说明】

订货:

 

 

IMMUNOPURE STREPTAVIDIN, ALKALINE PHOSPHATASE CONJUGATED品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

  •  

 

IMMUNOPURE STREPTAVIDIN,ALKALINE PHOSPHATASE CONJUGATED仅用于科研

 

Thermo Scientific Pierce Streptavidin and Conjugates include purified recombinant streptavidin biotin-binding protein, plus standard SA-HRP, SA-AP and fluorescent conjugates for biotin-based assays.

 

These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer.

 

Highlights:

  • Recombinant streptavidin – high-quality protein, 53kDa, pI = 6.8 to 7.5
  • Pure protein and enzyme conjugates – choose pure, unconjugated protein for biotin blocking and controls; enzyme conjugates for substrate-based detection
  • Fluorophore conjugates – standard fluorescent forms (fluorescein, rhodamine, etc.) for traditional immunofluorescence techniques
  • Activated preparations – choose a maleimide-activated or hydrazide-activated form to prepare a custom streptavidin conjugate with sulfhydryl- or carbohydrate-containing molecule

 

IMMUNOPURE STREPTAVIDIN, ALKALINE PHOSPHATASE CONJUGATED介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

UGATED现货供应

 

PIERCE

21324

IMMUNOPURE STREPTAVIDIN,ALKALINE PHOSPHATASE CONJUGATED, 1

1 MG

PIERCE

21327

EZ-Link(R) Sulfo-NHS-LC-Biotin, No-Weigh(tm) Format,

EA (8X1 MG)

PIERCE

21329

EZ-Link(R) NHS-PEG4-Biotin,   No-Weigh(tm) Format,

8 X 2 MG

21277-ProFound (tm) Pull-Down PolyHis Protein:Protein-Pierce试剂盒

【简单介绍】

货号:21277,名称:ProFound ™ Pull-Down PolyHis Protein:Protein,品牌:PIERCE,规格:KIT

【详细说明】

订货:

 

ProFound (tm) Pull-Down PolyHis Protein:Protein介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

产品特点:

 

ml树脂可结合10-25mg His标签融合蛋白

钴螯合树脂比镍螯合树脂对于多组氨酸标签更特异,产生更少的非特异性结合

说明书包含针对不同来源的诱饵和猎物蛋白的操作步骤

 

ProFound (tm) Pull-Down PolyHis Protein:Protein现货供应

 

PIERCE

21277

KIT

PIERCE

21324

IMMUNOPURE STREPTAVIDIN, ALKALINE PHOSPHATASE CONJUGATED, 1

1 MG

PIERCE

21327

EZ-Link(R) Sulfo-NHS-LC-Biotin, No-Weigh(tm) Format,

EA (8X1 MG)

 

品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

相关产品

 

产品货号

产品描述

包装

89964

HisPur钴树脂

用于纯化组氨酸标签蛋白。

介质为6%交联的微珠状琼脂糖l

瓶装,10ml

89965

HisPur钴树脂

用于纯化组氨酸标签蛋白。

介质为6%交联的微珠状琼脂糖

瓶装,100ml

89966

HisPur钴树脂

用于纯化组氨酸标签蛋白。

介质为6%交联的微珠状琼脂糖

瓶装,500ml

89967

HisPur钴树脂,离心柱型,0.2ml

用于快速纯化组氨酸标签蛋白。

介质为6%交联的微珠状琼脂糖

25个柱

 

仅用于科研