21435-PIERCE,EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit-琼脂糖

【简单介绍】

货号:21435,名称:EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit,品牌:PIERCE,规格:KIT

【详细说明】

订货:

 

EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

产品特点:

 

提供的试剂足以完成10次标记反应(货号:21455的试剂盒可以完成8次反应)。

每次反应可以标记1-10毫克蛋白,反应体系为0.5-2毫升。

• NHS-酯生物素专一地与伯胺反应(N-末端及赖氨酸残基的侧链)形成稳定的酰胺键。

• NHS酯的磺酸盐形式(或者是货号:21955的试剂盒中的PEG 间隔臂)促进生物素试剂的水溶性。

使用Zeba脱盐离心柱(5毫升)可以进行快速的样品净化和高效的蛋白回收速度比透析及传统的凝胶过滤更快,蛋白回收率和脱盐效率比超滤的更好

提供测定标记效率的试剂和方法。

 

EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit现货供应

 

PIERCE

21425

EZ-Link (R) Sulfo-NHS Biotinylation Kit

KIT

PIERCE

21435

EZ-Link (R) Sulfo-NHS-LC      Biotinylation Kit

KIT

PIERCE

21441

EZ-Link (R) NHS-SS-Biotin,    50 mg.

50 MG

 

EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit相关产品

 

产品货号

产品名称

包装

21217

Sulfo-NHS-Biotin§

也提供非磺化的透膜的类似物

50毫克

21326

Sulfo-NHS-Biotin,无需称量。

8 x 2毫克

21425

Sulfo-NHS-Biotinylation试剂盒

试剂盒

21925

微量Sulfo-NHS-Biotinylation试剂盒

试剂盒

 

EZ-Link (R) Sulfo-NHS-LC Biotinylation Kit

仅用于科研

 

21425-PIERCE,EZ-Link (R) Sulfo-NHS Biotinylation Kit-琼脂糖

【简单介绍】

货号:21425,名称:EZ-Link (R) Sulfo-NHS Biotinylation Kit,品牌:PIERCE,规格:50 MG

【详细说明】

订货:

 

EZ-Link (R) Sulfo-NHS Biotinylation Kit介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (R) Sulfo-NHS Biotinylation Kit现货供应

 

PIERCE

21425

EZ-Link (R) Sulfo-NHS Biotinylation Kit

KIT

PIERCE

21435

EZ-Link (R) Sulfo-NHS-LC      Biotinylation Kit

KIT

PIERCE

21441

EZ-Link (R) NHS-SS-Biotin,    50 mg.

50 MG

 

EZ-Link (R) Sulfo-NHS Biotinylation Kit品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (R) Sulfo-NHS Biotinylation Kit仅用于科研

 

Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

 

EZ-Link Sulfo-NHS-Biotin Reagents enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.

 

Highlights:

  • Label almost any antibody with biotin to facilitate immobilization, purification or detection
  • Short spacer arm (13.5 angstroms) adds minimal mass to tagged molecules (226.3 daltons)
  • Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
  • Sulfonate group attached to the biotinylation reagents increases reagent water solubility compared to ordinary NHS esters
  • Sulfo-NHS-Biotin is water soluble, so biotin labeling can be done under physiologic conditions
  • High purity, crystalline biotin labeling reagents can be used to create high-quality biotin-labeled derivatives

  •  

21360-EZ-Link (R) Biotin-PEG4-Hydrazide-琼脂糖

【简单介绍】

货号:21360,名称:EZ-Link (R) Biotin-PEG4-Hydrazide,品牌:PIERCE,规格:50 MG

【详细说明】

订货:

 

EZ-Link (R) Biotin-PEG4-Hydrazide介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (R) Biotin-PEG4-Hydrazide现货供应

 

PIERCE

21360

, 50 mg.

50 MG

PIERCE

21425

EZ-Link (R) Sulfo-NHS Biotinylation Kit

KIT

PIERCE

21435

EZ-Link (R) Sulfo-NHS-LC      Biotinylation Kit

KIT

 

品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

仅用于科研

 

Thermo Scientific EZ-Link Hydrazide-PEG4-Biotin (also called Biotin-PEG4-Hydrazide) is an aldehyde-reactive biotinylation reagent that contains a 4-unit polyethylene glycol (PEG) spacer arm for increased hydrophilicity.

Biotin-PEG4-Hydrazide enables biotinylation of glycoproteins and other molecules containing aldehydes or oxidizable sugar groups. The hydrazide group reacts spontaneously with aldehyde or ketone groups to form a stable hydrazone bond. Aldehyde groups can be created by gentle periodate oxidation of cis-diols in sialic acid and other sugars in carbohydrates. The hydrophilic, 4-unit polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG segment also adds length and flexibility to the spacer arm, minimizing steric hindrance involved with binding to avidin molecules.

Highlights:

  • Carbohydrate-reactive – forms stable hydrazone bond with periodate-oxidized sialic acid and other sugar groups in glycoproteins
  • Good choice for labeling polyclonal antibodies in the carbohydrate-rich Fc region, thereby avoiding steric hindrance of the antigen-binding sites
  • Use to biotinylate cell surface glycoproteins
  • PEGylation increases solubility and helps prevent aggregation of labeled molecules
  • PEG spacer arm reduces steric hindrance when binding to avidin molecules
  • Reactive group: hydrazide
  • Reactive toward: aldehyde and ketone groups (oxidized cis-diols in glycoproteins)

21343-PIERCE,EZ-Link (tm) Biotin-HPDP-琼脂糖

【简单介绍】

货号:21343,名称:EZ-Link ™ NHS-LC-LC-Biotin,品牌:PIERCE,规格:50 MG

【详细说明】

订货:

 

EZ-Link (tm) NHS-LC-LC-Biotin介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) NHS-LC-LC-Biotin现货供应

 

PIERCE

21343

EZ-Link (tm) NHS-LC-LC-Biotin

50 MG

PIERCE

21360

EZ-Link (R) Biotin-PEG4-Hydrazide, 50 mg.

50 MG

PIERCE

21425

EZ-Link (R) Sulfo-NHS Biotinylation Kit

KIT

 

EZ-Link (tm) NHS-LC-LC-Biotin品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (tm) NHS-LC-LC-Biotin仅用于科研

 

Thermo Scientific EZ-Link NHS-LC-LC-Biotin is a doubly long-chain, NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for intracellular labeling.

 

This is one of three EZ-Link NHS-Biotin Reagents that enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution. Differing only in their spacer arm lengths, the three reagents offer researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.

 

Highlights:

  • Lysine-reactive – label any antibody or other protein with biotin for use in protein methods
  • Irreversible – forms stable amide bonds that are permanent
  • Solubility – must be dissolved in DMSO or DMF before further dilution in aqueous buffers
  • Long reach – spacer arm (total length added to target) is 30.5 angstroms

     

     

  • Membrane-permeable – can be used to label inside cells (intracellular)

     

21341-PIERCE,EZ-Link (tm) Biotin-HPDP-琼脂糖

【简单介绍】

货号:21341,名称:EZ-Link ™ Biotin-HPDP,品牌:PIERCE,规格:50 MG

【详细说明】

订货:

 

EZ-Link (tm) Biotin-HPDP介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) Biotin-HPDP现货供应

 

PIERCE

21341

EZ-Link (tm) Biotin-HPDP,     50 Mg.

50 MG

PIERCE

21343

EZ-Link (tm) NHS-LC-LC-Biotin

50 MG

PIERCE

21360

EZ-Link (R) Biotin-PEG4-Hydrazide, 50 mg.

50 MG

 

EZ-Link (tm) Biotin-HPDP品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (tm) Biotin-HPDP

仅用于科研

 

Thermo Scientific EZ-Link HPDP-Biotin is pyridyldithiol-activated, sulfhydryl-reactive biotinylation reagent that conjugates via a cleavable (reversible) disulfide bond to enable use in a variety of purification methods.

 

Highlights:

  • Pyridyldithiol reacts with -SH groups to form a stable disulfide bond
  • Pyridine-2-thione leaving group can be used to measure degree of biotinylation at 343nm
  • Disulfide bond is cleavable using 50mM DTT, or 100mM beta-mercaptoethanol
  • Extender spacer arm prevents steric hindrance
  • Must be dissolved in DMSO or DMF before use
  • Biotinylation occurs between pH 6 to 9

  •  

21340-PIERCE,EZ-Link (tm) Biotin-LC-Hydrazide-琼脂糖

【简单介绍】

货号:21340,名称:EZ-Link ™ Biotin-LC-Hydrazide,品牌:PIERCE,规格:50 MG

【详细说明】

订货:

 

EZ-Link (tm) Biotin-LC-Hydrazide介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) Biotin-LC-Hydrazide现货供应

 

PIERCE

21336

EZ-Link (tm) NHS-LC-Biotin

50 MG

PIERCE

21338

EZ-Link (tm) Sulfo-NHS-LC-LC-Biotin

50 MG

PIERCE

21340

EZ-Link (tm) Biotin-LC-Hydrazide, 50 Mg.

50 MG

 

EZ-Link (tm) Biotin-LC-Hydrazide品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (tm) Biotin-LC-Hydrazide仅用于科研

 

Thermo Scientific EZ-Link Hydrazide-Biotin is the shortest and simplest hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes.

 

Thermo Scientific EZ-Link Hydrazide-LC-Biotin is a mid-length, simple, hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes.

 

These biotin hydrazide reagents enable simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of antibodies with sodium periodate produces reactive aldehydes on the carbohydrate moieties of the Fc portion that can be modified by hydrazides. This approach is advantageous for labeling antibodies because biotinylation occurs only at the sites of glycosylation, which are primarily in the Fc region of the antibody, far from the antigen binding site.

 

Highlights:

  • Label glycoproteins with biotin to facilitate immobilization, purification or detection
  • Attaches a biotin label to antibodies through carbohydrate modifications in the Fc region
  • Biotin hydrazides react with oxidized carbohydrates (aldehydes) at pH 4 to 6 to attach biotin through stable hydrazone bonds
  • Biotin hydrazides can be used to specifically label cell surface glycoproteins

21336-PIERCE,EZ-Link (tm) NHS-LC-Biotin-琼脂糖

【简单介绍】

货号:21336,名称:EZ-Link ™ NHS-LC-Biotin,品牌:PIERCE,规格:50 MG

【详细说明】

订货:

 

EZ-Link (tm) NHS-LC-Biotin介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) NHS-LC-Biotin现货供应

 

PIERCE

21336

EZ-Link (tm) NHS-LC-Biotin

50 MG

PIERCE

21338

EZ-Link (tm) Sulfo-NHS-LC-LC-Biotin

50 MG

PIERCE

21340

EZ-Link (tm) Biotin-LC-Hydrazide, 50 Mg.

50 MG

 

EZ-Link (tm) NHS-LC-Biotin品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

 

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (tm) NHS-LC-Biotin仅用于科研

 

Thermo Scientific EZ-Link NHS-LC-Biotin is a long-chain, NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for general intracellular labeling.

 

This is one of three EZ-Link NHS-Biotin Reagents that enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution. Differing only in their spacer arm lengths, the three reagents offer researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.

 

Highlights:

  • Lysine-reactive – label any antibody or other protein with biotin for use in protein methods
  • Irreversible – forms stable amide bonds that are permanent
  • Solubility – must be dissolved in DMSO or DMF before further dilution in aqueous buffers
  • Medium length – spacer arm (total length added to target) is 22.4 angstroms
  • Membrane-permeable – can be used to label inside cells (intracellular)

  •  

21335-PIERCE,EZ-Link (tm) Sulfo-NHS-LC-Biotin-琼脂糖

【简单介绍】

货号:21335,名称:EZ-Link ™ Sulfo-NHS-LC-Biotin, 100 mg,品牌:PIERCE,规格:100 mg

【详细说明】

订货:

 

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg现货供应

 

PIERCE

21335

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg

100 MG

PIERCE

21336

EZ-Link (tm) NHS-LC-Biotin

50 MG

PIERCE

21338

EZ-Link (tm) Sulfo-NHS-LC-LC-Biotin

50 MG

 

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

 

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg

仅用于科研

 

Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

 

EZ-Link Sulfo-NHS-Biotin Reagents enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.

 

Highlights:

  • Label almost any antibody with biotin to facilitate immobilization, purification or detection
  • Intermediate spacer arm (22.4 angstroms) provides excellent balance of adequate length (minimal steric hindrance for biotin binding) and modest mass (339.5 daltons)
  • Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
  • Sulfonate group attached to the biotinylation reagents increases reagent water solubility compared to ordinary NHS esters
  • Sulfo-NHS-LC-Biotin is water soluble, so biotin labeling can be done under physiologic conditions
  • High purity, crystalline biotin labeling reagents can be used to create high-quality biotin-labeled derivatives

  •  

企业内控号LTS1077006-人外周血淋巴细胞分离液(用于生物治疗细胞的分离) 内毒素<0.06EU 医用级-细胞分离液

【简单介绍】

货号:HY2015(企业内控号:LTS1077006) ,产品名称:样本密度分离液(人外周血淋巴细胞分离液)(医用级分离液,用于生物治疗细胞的分离) 内毒素<0.06EU 医用级 ,品牌:TBD,规格:200ml/瓶

【详细说明】

货号:HY2015(企业内控号:LTS1077006) ,产品名称:样本密度分离液(人外周血淋巴细胞分离液)(医用级分离液,用于生物治疗细胞的分离) 内毒素<0.06EU 医用级 ,品牌:TBD,规格:200ml/瓶

  

21331-EZ-Link (tm) Sulfo-NHS-SS-Biotin, 100 mg-琼脂糖

【简单介绍】

货号:21331,名称:EZ-Link ™ Sulfo-NHS-SS-Biotin, 100 mg,品牌:PIERCE,规格:100 mg

【详细说明】

订货:

 

EZ-Link (tm) Sulfo-NHS-SS-Biotin, 100 mg介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) Sulfo-NHS-SS-Biotin, 100 mg现货供应

 

PIERCE

21331

100 MG

PIERCE

21335

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg

100 MG

PIERCE

21336

EZ-Link (tm) NHS-LC-Biotin

50 MG

 

品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

 

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

仅用于科研

 

Thermo Scientific EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

Sulfo-NHS-SS-Biotin is a thiol-cleavable amine-reactive biotinylation reagent that contains an extended spacer arm to reduce steric hindrances associated with avidin binding. This reagent is water-soluble, enabling biotinylation to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents or are complicated by their inclusion.

Highlights:

  • Label almost any protein with biotin to facilitate immobilization, purification or detection
  • Ideal for reversible biotinylation; disulfide bond in the spacer arm allows the biotin label to be removed as desired
  • Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
  • Sulfonate group attached to the biotinylation reagent increases water solubility to a minimum of 6 mg/ml, so biotin labeling can be done under physiologic conditions
  • Long spacer arm on the biotin reduces steric hindrance when binding to avidin molecules