[1]. Liu B, et al. Diosmetin induces apoptosis by upregulating p53 via the TGF-β signal pathway in HepG2 hepatoma cells. Mol Med Rep. 2016 Jul;14(1):159-64. [2]. Yu G, et al. Diosmetin ameliorates the severity of cerulein-induced acute pancreatitis in mice by inhibiting the activation of the nuclear factor-κB. Int J Clin Exp Pathol. 2014 Apr 15;7(5):2133-42.
金雀异黄素抑制血清刺激的MCF-7和T47D ER +细胞的生长，分别通过染料排除分别为7.6和8.7μg/ mL，通过[3H]胸苷掺入分别为8.7和10.6μg/ mL。这些值类似于分别用MTT测定法获得的MCF-7和T47D ER +细胞的IC50值9.4和7μg/ mL。此外，与孵育8小时的对照细胞相比，浓度高达20μg/ mL的染料木黄酮不会改变MTT线粒体减少。此外，发现生物素A或大豆苷元都不会干扰IC50浓度下的MTT测定。因此，在研究的系统中，MTT分析对于确定Genistein在20μg/ mL浓度下的生长抑制作用是有效的[1]。
[1]. Peterson G, et al. Genistein inhibits both estrogen and growth factor-stimulated proliferation of human breast cancer cells. Cell Growth Differ. 1996 Oct;7(10):1345-51. [2]. Jalili C, et al. Effect of Genistein on reproductive parameter and serum nitric oxide levels in morphine-treated mice. Int J Reprod Biomed (Yazd). 2016 Feb;14(2):95-102. [3]. Ding S, et al. Environmentally Relevant Dose of Bisphenol A Does Not Affect Lipid Metabolism and Has No Synergetic or Antagonistic Effects on Genistein’s Beneficial Roles on Lipid Metabolism. PLoS One. 2016 May 12;11(5):e0155352
To investigate the endocytosis mechanism involved in the uptake process, cells were pre-treated with endocytic inhibitors including chlorpromazine (100 μM), genistein (200 μM), and amiloride (200 µM) for 30 min. Ten the cells were treated with 1 μg of FITC-labeled DMSNs for 6 h. In a control group, cells were not treated with the inhibitors prior to nanoparticle treatment. Cellular uptakes of nanoparticles were quantitated by fow cytometer as described above.
来源文献：Mo C, Lu L, Liu D, Wei K. Development of erianin-loaded dendritic mesoporous silica nanospheres with pro-apoptotic effects and enhanced topical delivery. J Nanobiotechnology. 2020 Mar 30;18(1):55. doi: 10.1186/s12951-020-00608-3. PMID: 32228604; PMCID: PMC7104482.
