分类目录归档：日本 Jaica老化

日本 JaICA老化Anti 4-HNE monoclonal antibody (HNEJ-2)

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日本 JaICA老化Anti 4-HNE monoclonal antibody (HNEJ-2)
日本 JaICA 老化 Antibody系列产品
上海金畔生物作为日本 JaICA 老化中国代理商，欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
抗4-羟基壬烯醛单克隆抗体
Anti 4-hydroxy-2-nonenal(4-HNE) monoclonal antibody(HNEJ-2)
货号：MHN-100P
品牌：JaICA
规格： 100µg
产品说明 / Product Overview：
Anti 4-HNE monoclonal antibody (HNEJ-2)

Structure of 4-hydroxy-2-nonenal
 
脂质氧化标志物
适用于免疫组织化学和免疫印迹.（仅供研究使用）
关于4-羟基壬烯醛（4-hydroxy-2-nonenal; 4-HNE）
活性氧（ROS）参与活体内的脂质过氧化，膜脂质是ROS的主要靶标之一。 ROS在过氧化过程中形成各种醛。 4-羟基-2-壬烯醛（4-HNE）是一种α，β不饱和醛，可以通过omega-6不饱和脂肪酸（例如亚油酸和花生四烯酸）的过氧化形成。 特别需要指出的是，据报道在活体内形成的4-HNE源自磷脂结合的花生四烯酸。 4-HNE可能是脂质过氧化最可靠的生物标志物之一。
大肠癌细胞的高倍视图：腺癌（箭头所示）和非肿瘤性上皮细胞(star x240)。
(S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)

技术参数
克隆号＃：HNEJ-2；
免疫原：4-HNE修饰的钥孔血蓝蛋白(KLH)；
亚基：小鼠IgG1（kappa）；制备为腹水，并纯化硫酸铵；
性状：冻干粉（含20μg或100μg IgG）；
特异性：该抗体对于用其它醛（如2-壬烯醛，2-己烯醛，1-己醛，4-羟基-2-己烯醛，甲醛或戊二醛）处理的蛋白质显示出几乎可以忽略的反应性；
应用：免疫组织化学和免疫印迹；
储存：-20°C以下5年；运输时，室温下至少可稳定保持7天。

Clone #: HNEJ-2
Immunogen: 4-HNE-modified Keyhole Limpet Hemocyanin
Subclass: Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified.
Form: Lyophilized Powder (containing 20 碌g or 100 碌g of IgG)
Specificity: This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.
Applications: Immunohistochemistry and western blotting.
Storage: Less than -20掳C for 5 years. When transport, stable at least 7 days at room temperature.

References

Immunohistochemistry:
T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997)
Western blotting:
S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995)
Product name Code Content
Anti 4-HNE monoclobal antibody MHN-020P 20 ug of IgG/ vial
MHN-100P 100 ug of IgG/ vial

使用说明书
免疫组织化学操作方法
技术资料

日本JaICA Test kit for Potential Anti Oxidant (PAO)

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日本JaICA Test kit for Potential Anti Oxidant (PAO)
日本老化制御研究所 Jaica代理
上海金畔生物作为日本 JaICA 老化中国代理商，欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
Suitable for detection of total antioxidant capacity in serum and food extracts. For research use only.
Antioxidant assay:

Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.
Principle of this assay:

In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++ to Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.
Specifications:

Method: Colormetric assay(detection: 480 – 492 nm)
Assay range: 21.9 – 4378 碌mol/L (cupric ion reducing power)
Format: 96 wells
Storage: Room temperature (10 – 25掳C)
Applications: Human and animal serum samples, foods and beverage samples.
Required but not provided: A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are prepared with distilled water only).

 
Content of this kit: 试剂盒组成

Standard (Uric acid powder): 1 vial
Sample diluent: 1 bottle
Cu++ solution: 1 bottle
Stop solution: 1 bottle
Micro titer plate: 1 plate (96 wells)

Assay procedure:

1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 碌L of Sample Diluent, and add 10 碌L of standards or diluted samples.
3) Pour 200 碌L of mixture to Micro titer plate. Use 200 碌L of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 碌L of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 碌L of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 – READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (碌mol/L).
1mM of uric acid = 2189 碌mol/L (copper reducing power)

References

1) Oxidative imbalance and cathepsin D changes as peripheral blood biomarkers of Alzheimer disease: A pilot study
E Strafacea, P Matarresea, L Gambardella, R Vona, A Sgadari,MC Silveri, W Malorni
FEBS Letters 579, p2759-766 (2005)
2) Oxidative stress and its association with coronary artery disease and different atherogenic risk factors
C. VASSALLE, L. PETROZZI , N. BOTTO, M. G. ANDREASSI and G. C. ZUCCHELLI
Journal of Internal Medicine 256, p308-315(2004)
3) Antioxidant capacity as a reliable marker of stress in dairy calves transported by road
P Pregel, E Bollo, FT Cannizzo, B Biolatti, E Contato, and PG Biolatti
Veterinary Record 156, p53-54 (2005)
4) Vitamin E-coated dialyzers reduce oxidative stress related proteins and markers in hemodialysis ? a molecular biological approach.
LA Calo, A Naso, E Pagnin, PA Davis, M Castoro, R Corradin, P Riegler, C Cascone, W Huber and A Piccoli
Clinical Nephrology, Vol.62(5), p355-361 (2004)
5) Oxidative stress-related factors in Bartter’s and Gitelman9s syndrome: relevance for angiotensin IIsignalling.
Calo LA, Pagnin E, Davis PA, Sartori M, Semplicini A.
Nephrol Dial Transplant 18(8) p1518-1525 (2003)
6) Effect of epoetin on HO-1 mRNA level and plasma antioxidants in hemodialysis patients.
Calo LA, Stanic L, Davis PA, Pagnin E, Munaretto G, Fusaro M, Landini S, Semplicini A, Piccoli A.
Int. J Clin. Ther 41(5), p187-192 (2003)
7) Restored Antioxidant Capacity Parallels the Immunologic and Virologic Improvement in Children with Perinatal Human Immunodeficiency Virus Infection Receiving Highly Active Antiretroviral Therapy.
M Martino, F Chiarelli, M Moriondo, M Torello, C Azzari, and L Galli
Clinical Immunology 100(1),p82-86 (2001)
Product name Code Assay range Assay time Format
Test kit for Potential Anti Oxidant (PAO) KPA-050 21.9-4378 碌mol/L 5 minutes 96 wells

日本JaICA Test kit for Potential Anti Oxidant (PAO)   说明书  技术资料

Instruction manual Technical information

日本 JaICA 老化Anti Acrolein (ACR) monoclonal antibody

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日本 JaICA 老化Anti Acrolein (ACR) monoclonal antibody
上海金畔生物作为日本 JaICA 老化中国代理商，欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
Anti Acrolein (ACR) monoclonal antibody.
Suitable for immunohistocheistry. For research use only.
Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. ACR is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with ACR and lysine residue leads to the formation of numerous numbers of adducts, such as formyl-dehydropiperidino-lysine (FDP-lysine) type derivative. This antibody is specific for the ACR-modified protein, especially FDP-lysine type derivative.

Specification

Clone #: 5F6
Antigen: ACR-modified keyhole-lympet hemocyanine
Form: Frozen (100 碌g/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A.
Application: Immunohistochemistry.
Recommended antibody concentration is 0.5-1.0 碌g/mL on paraformaldehyde fixed tissue.
Specificity: Specific for ACR-modified protein (especially FDP-lysine type derivative)
Subclass: Mouse IgG1,kappa
Storage: Less than-20°C

抗丙烯醛单克隆抗体适用于免疫组织化学。
丙烯醛（ACR）是在环境中普遍存在并且通过氧化反应（例如脂质过氧化和髓过氧化物酶催化的氨基酸氧化）内源性形成的代表性致癌醛。 ACR是高度反应性的醛，并与蛋白质中的赖氨酸残基反应。与ACR和赖氨酸残基的反应导致大量的加合物的形成，例如formyl-dehydropiperidino-lysine（FDP-赖氨酸）类型衍生物。
该抗体对ACR修饰的蛋白，特别是FDP-赖氨酸类型衍生物具有特异性。
技术参数
克隆＃：5F6；
抗原：ACR修饰的钥孔血蓝蛋白；
形式：冻结（100μg/ mL抗体在含有0.1％NaN3和0.5％BSA的10mM PBS中）。通过蛋白A纯化；
应用：免疫组织化学；
推荐抗体浓度：0.5-1.0μg/ mL对于多聚甲醛固定的组织；
特异性：特定针对ACR修饰的蛋白（特别是FDP-赖氨酸类型衍生物）；
亚型：小鼠IgG1，kappa；
储存：小于-20°C

References
1)Protein-bound acrolein: Potential markers for oxidativestress. K.Uchida, M.Kanematsu, K.Sakai, T.Matsuda, N.Hattori,Y.Mizuno, D.Suzuki,T.Miyata, N.Noguchi, E.Niki,T.Osawa
Proc.Natl.Acad.Sci.USA,95,4882-4887(1998)
2)Protein-bound acrolein: A novel markers of oxidativestress in Alzheimer’s Disease. Noel Y. Calingasan, Koji Uchida, and Gary E.Gibson
Journal of Neurochemistry.72(2),751-756(1999)

Product name Code Content
Anti Acrolein monoclonal antibody MAR-020n 20 ug of IgG
MAR-100n 100 ug of IgG

日本 JaICA 老化Anti Acrolein monoclonal antibody
抗丙烯醛（ACR）单克隆抗体 使用使用说明书
技术资料