日度归档：2024年12月23日

蛋白预制胶Hepes-Tris, 15％, 15孔, 1.5 mm

发表于由

蛋白预制胶Hepes-Tris, 15％, 15孔, 1.5 mm

货号：BL595A

规格：10块/盒

品牌： biosharp

商品详情：

Precast Gel, Hepes-Tris, 15％, 15 wells, 1.5 mm

蛋白预制胶Hepes-Tris, 15％, 15孔, 1.5 mm

产品编号

产品名称

规格

BL595A

蛋白预制胶Hepes-Tris, 15％, 15孔, 1.5 mm

10块

 

产品简介：

Jinpan蛋白预制胶Hepes-Tris是一款性能理想的凝胶，适用于多种样本类型和宽范围分子量的蛋白。它们可提供清晰笔直的条带，带有易于上样的高容量上样孔，可用于变性或非变性凝胶电泳应用。

 

产品特点：

用途多样—可用于变性和非变性蛋白的分析

性能稳定—自动化的灌胶生产技术，确保产品质量稳定性和重复性

玻璃胶板—有效减少蛋白非特异性吸附，蛋白条带尖锐，清晰

兼容性广—适用BIORAD，Invitrogen，六一，天能和君意东方等品牌mini电泳槽

缩短时间—恒压下快速分离蛋白质，只需40-50 min

保质期长—在4℃下保存凝胶可长达18个月

 

基本信息：

胶板尺寸(宽×高×厚)

98×84×4.1 mm

凝胶厚度

1.5 mm

凝胶尺寸(宽×高×厚)

81×74×1.5 mm

孔数

15孔

浓缩胶（浓度，高度）

4%，1.5 cm

最大上样量

30 μL

分离胶（浓度）

15%

缓冲体系

Hepes体系

 

使用方法：

1. 请根据需求选择合适浓度的预制胶，以便进行更好的蛋白电泳条带分离。

2. 将蛋白预制胶从包装袋中取出。

3. 将蛋白预制胶固定在电泳槽中。

4. 在电泳槽中加入电泳液（内槽加满电泳液，外槽电泳液最低须加到1/3液面处，最高不可超过胶板），再缓慢将梳子拔出，用移液器吸取电泳液轻轻吹打加样孔，去除加样孔内残余的储存缓冲液。（每盒胶赠送10包电泳缓冲液粉末，每包粉末可用500 mL去离子水进行溶解，得到500 mL 1×电泳液。）

5. 将混合好loading buffer（变性还原：BL502；变性非还原：BL511；非变性非还原：BL529）的蛋白样品加入上样孔。注意枪头不要戳破凝胶，不要过度插入梳孔使胶板变形造成漏夜。

6. 建议电泳条件：150 V，40~50 min，当溴酚蓝指示带电泳至胶板底部，或实验预定位置时，即可结束电泳。

7. 取出凝胶，用刀在侧边硅胶处，沿着两片玻璃缝隙切开硅胶，即可打开玻璃板。取胶时，需在胶和玻璃条之间，沿着玻璃条划一刀，防止发生粘连。

 

注意事项：

1. 蛋白预制胶Hepes-Tris使用的是中性的Hepes缓冲系统，请勿使用Tris-Glycine等其他电泳缓冲液进行电泳。

推荐使用赠送的Hepes电泳缓冲液，或根据以下配方自行配制：

变性胶缓冲液配方

非变性胶缓冲液配方

50 mM Tris

50 mM Tris

50 mM HEPES

50 mM HEPES

0.1% SDS

2 mM EDTA

2 mM EDTA

2. 如果需要蛋白条带更加清晰、平直，可降低电压至100-120V，适当延长电泳时间。

3. 电压为150V电泳时，1块胶的电流在80mA左右，2块胶的电流在140mA左右，随着时间增加电流会逐步降低。

4. 如要重复使用电泳缓冲液，建议每次更换内槽电泳缓冲液，外槽根据电泳实际情况更换。为了保证最佳电泳效果，不建议重复使用电泳缓冲液。

5. 电泳结束后可以使用Tris-Glycine转膜液进行转膜。将凝胶浸泡在转膜液中10-15 min，使凝胶中的缓冲液得到充分平衡，再进行转膜。

6. 湿转时120V恒压转膜60-90 min。为达到更好的转膜效果，可以根据预制胶上残留的预染marker及膜上的预染marker确定转膜效率，并对转膜条件进行适当调整。目的蛋白的分子量，凝胶浓度及转膜液中的甲醇浓度都会影响转膜效率。大蛋白尽量选择低浓度的胶。蛋白分子量100KD以上，建议甲醇浓度5%；10-100KD，建议甲醇浓度10%；10KD以下，建议甲醇浓度20%-30%。

7. 由于本预制胶改进了BIORAD的mini胶板两侧上端与硅胶垫接触的凹陷结构，使其兼容所有厂家的mini胶电泳槽。使用时需将BIORAD的绿色硅胶封闭垫取出后反过来安装，使其没有凸起的平滑面朝外，防止漏液。使用Life的电泳槽时，需配合特制挡板一起使用，请联系经销商索取。

8. 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。

9. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

 

保存条件：

1. 4℃储存，可存放18个月；常温下储存，可存放12个月。

2. 请勿置于0℃以下，以免凝胶发生冻裂。

3. 常温运输；冬季泡沫盒保温运输。

货号 BL595A
规格 10块/盒
品牌 biosharp
说明书下载 点击下载

0155-500G-8-HYDROXYQUINOLINE-amresco试剂

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【简单介绍】

货号：0155-500G，产品名称：8-HYDROXYQUINOLINE，品牌：Amresco，规格：500G

【详细说明】

0155
8-HYDROXYQUINOLINE
Grade:ACS GRADE
CAS Number: 148-24-3Synonym(s): None
Molecular Weight: 145.16Molecular Formula: C9H7NO
Storage Condition: ROOM TEMPERATURE
CodeSizePriceQuantity
0155-100G
0155-250G
0155-500G
Helps to stabilize phenol and retards the formation of phenol oxidation products.

   

olympus奥林巴斯工业显微镜OLYMPUS Stream图像分析软件

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olympus奥林巴斯工业显微镜OLYMPUS Stream图像分析软件

olympus奥林巴斯工业显微镜OLYMPUS Stream图像分析软件

产品编号：
市场价：￥0.00元
会员价：￥0.00元
品牌：Olympus奥林巴斯
生产厂家：olympus奥林巴斯

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OLYMPUS Stream Designed with Your Workflow in Mind

Keep Your Workflow Streamlined

BX51M and OLYMPUS Stream Your time is just as important as your working conditions. That’s why the OLYMPUS Stream system can be personalized to fit your process flow. An easy-to-use interface guides you effortlessly through every step from image adjustment, image capture, measurements, report creations and data basing or whatever end result you need to achieve. As a result, you’ll find that you can complete your tasks more efficiently regardless of the complexity.

Build Your Olympus System Your Way

The OLYMPUS Stream software system can be purchased in a variety of packages designed to fit your needs. Whether you require the capabilities found in the entry level OLYMPUS Stream Start or the advanced automation controls found in OLYMPUS Stream Motion. The packages are also expandable with specific applications modules to meet unique analysis needs. There is always the right package for your requirements. As a result, you can have your system exactly as you wish – your way.

Easily Expandable To Future Applications

With the OLYMPUS Stream system, you’re prepared not only for today’s applications but for tomorrow’s. Whatever the future may bring, you’ll be ready to keep up with advances in technology with OLYMPUS Stream system solutions.

Streamlined User Interface

User Interface Concept

OLYMPUS Stream user interface As you progress from image capture to report creation, the tool windows you need are always displayed at every stage. You can quickly and easily access control parameters.

Dynamic User Interface

Depending on your needs, you can arrange the layout of the tool window to best fit your workflow. You can create customized layouts with all the necessary functions for each activity.

Microsoft Office Integration

OLYMPUS Stream can create professional reports directly into Microsoft Word. Report templates and headers can be flexibly modified according to your company’s needs. The images can be zoomed and annotated along with the metadata.
Statistical analysis and graphing are also accomplished using Excel with the output easily imported into your report. OLYMPUS Stream uses a unique compression method that allows you to store view reports without the need for any unique applications software.

 

My Function

You can create intuitive workflows based on the most frequently used functions, simplifying repetitive tasks so that even new users are able to operate the software easily and efficiently.

OLYMPUS Stream Adapts to Your Workflow

Live Image Navigation

Using the OLYMPUS Stream image navigation allows you to pan and zoom on live or stored images. The image navigator interface provides immediate updates at your location on both the zoomed and unmagnified images. The navigation system provides you with fast, immediate views of your sample before you acquire an image. All image viewing and manipulation is carried out with a clean, simple user interface, providing fast and intuitive results.


Peeled integrated circuit

Optimized Focus and Exposure

The Olympus Stream focus indicator enables users to select a region of interest and bring it into optimum focus using the focus control of the microscope. This function is essential when a large optical depth of field makes it difficult to find the best focus position by eye. OLYMPUS Stream’s, live histogram display and over-exposure indicator let you easily find the optimum exposure time to avoid over exposed images that cause a loss of detail. Your digital camera’s exposure time can then be adjusted manually or automatically when using the family of Olympus DP series cameras.

 

Instant Extended Focus Image (EFI)

OLYMPUS Stream software easily provides images for samples that extend beyond the depth of focus. The manual Extended Focus Image allows you to use the fine focus adjustment to combine many images in the z-axis to provide you with a single combined output that can be used for visualization or measuring in x and y.

Manual Multiple Image Alignment (MIA)

OLYMPUS Stream software provides MIA to enable the creation of panoramic images of samples that extend beyond the field of view. The simple step-by-step process allows you to quickly combine the images. The OLYMPUS Stream software then quickly stitches them together, providing you with an output ready for simple visualization or complex measurement.

Olympus Offers Solutions to Save You Time

Autofocus

For precise sample focusing and speedy image acquisition, OLYMPUS Stream offers two autofocus options. For surfaces with high contrast, OLYMPUS Stream can also use its contrast-based software autofocus. For surfaces with low contrast, such as polished or transparent materials, the laser autofocus unit is a fast, repeatable option when integrated with the Olympus BXiS microscope system. Both systems provide perfectly focused images with the simple click of a button.

Macro Recorder

OLYMPUS Stream provides a way to preset complex imaging and measuring tasks with the Macro Recorder. The Macro Recorder creates step-by-step processes, which, after completion, are stored and then implemented with a single click. This capability provides all operators with the same settings and consistent output.

Automatic MIA/EFI


Multiple Image acquisition Pt wires cross sections		 When creating large, high-resolution automated MIA (montage overview) images, the combination of OLYMPUS Stream with a motorized stage easily provides a fast result. The operator selects MIA scanning by using the stage navigator tool, which steps the operator though the entire process. When using the automatic EFI function with a motorized focus unit, the software recommends the optimum number of images required to provide perfectly focused images that are outside the depth of field.

Tilt Compensation

OLYMPUS Stream can predict a sample’s tilt by taking three or more points on the coordinate data when performing automated MIA using motorized systems. This shortens the time and increases the repeatability for stage height adjustment when acquiring images.

Gets You the Image You Want

3D Image Creation

By using the a motorized focus unit, images with different focus positions can be recorded automatically. In addition, calibrated z-profile measurements using z-stack data and 3D image creation using the 3D-EFI function are made possible. The three-dimensional view of the sample becomes crystal clear with a few mouse clicks.

Image Processing

OLYMPUS Stream has a variety of filters for edge detection, smoothing, and other purposes. You can visualize image features by enhancing and modifying with a rocessing filter on the acquired image. For best results, you can check or adjust the filter results in a preview display. Additionally, image manipulations such as subtraction and erosion are also easily carried out without losing your valuable original image.

From Simple Measurements to Particle Analysis

Basic Measurement


Measurement Operation		 Optical inspection and quality control require frequent measurements. OLYMPUS Stream provides interactive measurement functions such as distances, angles, rectangles, circles, ellipses and polygons. The measurements are made with your mouse and show immediate feedback indicated on the image or in the live data table. All results are saved with the image files as records. To further improve on accuracy, optical data such as system magnification are automatically available when combined with the Olympus Microscope system.

Measurement Result

Included with the OLYMPUS Stream Basic package and other more advanced packages is an integrated data analysis package. The data analysis provides statistical analysis on your data based on your needs. You can calculate mean standard deviation, min, max, and more. All of the data gathered is exported to Microsoft Excel with a single click.

Magic Wand

OLYMPUS Stream Essentials includes a wand for automatically detecting the edges of the sample you wish to measure. The Magic Wand allows you to select the measurement points-free hand for simple or multiple lines, plus free hand polygon measurements for irregularly shaped objects.

Layer Management

Each image as it is captured and annotated is broken into layers (original image, measurement data, annotations etc.), allowing you to change processing files (smoothing and/ or sharpening) without affecting your measurement results. You can easily retain your measuring data as you carry out your analysis and measurements.

Traceability

For quality control and traceability recording the conditions of image acquisition is essential. With OLYMPUS Stream, you can quickly create a calibration report for important information like magnification, objective lens type, pixel size, scale bar, etc. The Info Stamp created from updated calibration information can be used for overlaying on the acquired image and reports.

Phase Analysis

OLYMPUS Stream Essentials and other more-advanced packages offer a very flexible and powerful phase analysis tool with ROI selection. It speeds and simplifies the extraction of phase fraction data of more than one compound in the same image.

Count and Measure

Object Detection and Classification Object detection and size distribution measurement are among the most important applications in digital imaging. OLYMPUS Stream incorporates a detection engine that utilizes threshold methods to reliably separate objects (e.g. particles, scratches) from the background. OLYMPUS Stream offers more than 50 different parameters for shape, size, position, and pixel properties (intensity, gray value) for object classification.

Intuitive Workflow Interfaces

Easy-to-use Operation

With the click of an icon on the Materials Solution tool window, you can execute the most complex image analysis task quickly, precisely, and in compliance with most common international standards.

Example: Grains Planimetric

Olympus Broad Range of Digital Cameras

DP72

  With this color digital camera, resolution, sensitivity, frame rate, and color fidelity combine to provide the highest level of performance. The DP72 is compatible with all samples and observation modes, including fluorescence, and produces clear
12.8M-pixel sample images

DP25

This high-resolution 5M pixel color CCD camera provides the highest performance in brightfield observation for most applications. The DP 25 accommodates all techniques with high color fidelity.

DP21

This 2M-pixel color CCD camera can be controlled from a space-saving, intuitively operated hand- switch (PC required). The hand-switch incorporates the 12 most frequently used measurement functions for efficient inspection of industrial parts.

 

OLYMPUS Stream Expands to Meet Your Future Needs

Database Management

When you need to efficiently browse through thousands of images and other files created in the past, OLYMPUS Stream can streamline your workflow from image capture through data management.
The software incorporates a client-server database based on Microsoft SQL Server Express. It allows you to assign user-definable fields (creation date, project ID, parts number, deadline, and metadata) into image and other files and folders, permitting efficient data sharing and quick searches.

Simple Network Connections

The OLYMPUS Stream NETCAM option lets any authorized network user connect to your OLYMPUS Stream PC and visualize the same image in real time with a web browser. What’s more, the DP21 also provides self-contained integration into your local network, allowing you to share your work across your office or around the world.

Microsoft Office 2010

Stay up to date with OLYMPUS Stream. The report tool can use the new version of the very popular Microsoft Office suite. OLYMPUS Stream is there and is designed for the future.

Windows 7 64-bit

The 64-bit edition of Microsoft Windows 7 is the new reference in operating systems. OLYMPUS Stream fully utilizes all of the advanced capabilities of 64-bit computing for your everyday tasks. You can smoothly execute advanced image processing tasks (like counting more than 2 million objects simultaneously) that were impossible before.

Motion
Basic Image Acquisition
Live image acquisition Captures live image in various format ■ ■ ■ ■
Live pan and zoom Zoom into live image using mouse wheel, displays crosshair in overlay ■ ■ ■ ■
Basic Image Tools
Image history and properties Displays image history and properties ■ ■ ■ ■
Image navigator Enables tool window for image navigation and zooming ■ ■ ■ ■
Gallery view Displays thumbnails of open images in a gallery ■ ■ ■ ■
Layers Enables viewing, extraction and deletion of single image layers ■ ■ ■ ■
Image processing and filters Enables contract adjustment, edge detection, smoothing and sharpening of images and shading correction ■ ■ ■ ■
Static annotations Draws text, arrows, lines, rectangles and ellipses on the image ■ ■ ■ ■
Interactive Measurements
Field of view measurement Measures distances, angles, rectangles, circles, ellipses and polygons ■ ■ ■ ■
Advanced measurement Interpolated polygon, Magic wand – – ■ ■
Olympus Device Control
Olympus microscope control Controls motorised and coded Olympus microscope systems BX2, GX, SZX, SZX2 and MX, reads out Prior SZX-ZE ■ ■ ■ ■
Olympus cameras MX61A, MX61, MX61L, SZX-MDCU, SZX2-MDCU, BX-UCB, IX-UCB, BX-REMCB, U-CBS, Prior SZX-ZE ■ ■ ■ ■
Extended Image Acquisition
Movie Create movie files (*.avi format) – ■ ■ ■
Software autofocus Perform contrast-based sorftware autofocus3 – ■ ■ ■
Reporting
Data export and statistics Exports measurement data to Microsoft Excel and OLYMPUS Stream workbook, including statistical analysis of measurements – ■ ■ ■
Report composer Creates Microsoft Word documents, directly from OLYMPUS Stream (requires Microsoft Word 2003, 2007 or 2010) – ■ ■ ■
Extension to Microsoft Word Creates additional menu for report creation into Microsoft Word – ■ ■ ■
Basic Customization
MyFunctions Creates a workflow and large button bar for frequently used commands – ■ ■ ■
Extended Device Control
Non-Olympus cameras Controls non-Olympus cameras4 – ■ ■ ■
Imaging source converter Controls DFG-1394 A-D (CON 800) converter (FireWire and USB 2.0) – ■ ■ ■
Advanced Customization
Layout management Allows recording and editing of layouts – ■ ■ ■
Macro recorder Allows recording and editing of macros – – ■ ■
Advanced Acquisition Process
Instant EFI Instantly creates an EFI image while focusing – Option ■ ■
Manual MIA Creates panoramic images over areas (requires manual XY stage) – Option ■ ■
Time lapse Captures still images over time frequency – – ■ ■
Extended Image Tools
Image arithmetic Performs arithmetic and logical operations with image – – ■ ■
Basic Image Analysis
Phase analysis Perform threshold-based phase segmentation on full images and ROIs5, calculates area, area fraction and objects count – – ■ ■
Advanced Automated Acquisition Process
Automated EFI Automatically creates an EFI image via a predefined number of frames, step size and top/bottom range (requires motorised Z) – – – ■
Automated MIA Creates panoramic images over areas (requires motorised stage) – – – ■
Automated Z-stack acquisition Automatically acquires Z-stacks (requires motorised Z) – – – ■
Stage navigator Position list and stage navigator to capture images at several stage positions or over stage areas – – – ■
Stage Control
3rd party stage controls Controls XY stage controllers from Objective Imaging, Prior ProScan, Ludl MAC, Märzhauser, ITK and LANG – – – ■
Advanced Image Tools
Intensity calibration Performs intensity calibration of channels – – – ■
Z-stacks projection Calculates projections over Z-Stacks – – – ■
Data Management
Client-server database Provides image and data management solution for microscopy (utilises Microsoft SQL Server Express 2005 or 2008) – – – ■
User rights management Provides user rights management for granting access to different records and record types – – – –
Large Database and LIMS support Support of Oracle 11g and Microsoft SQL databases and allow the communication with external LIMS – – – –

1. Altra 20, DP20, DP21, DP25, DP26, DP70, DP71, DP72, SC20, SC30, SC100, XC10, XM10, XM10IR, UC30, UC50, XC30, XC50
2. CC12, CVI, CVII, CVIII, CVIIIu, F-View II
3. Requires Olympus microscope with motorized Z-axis or external motorised Z-axis (supported in OLYMPUS Stream Motion)
4. Qimaging cameras: MicroPublisher 3.3 RTV, MicroPublisher 5 RTV; Jenoptik cameras: ProgRes C3, ProgRes CT3, ProgRes C5
5. Rectangle, circle, polygons and magic wand.

Materials Solution
Layer Thickness Measurement
Description Measures the thickness of single or multiple layers (cross section view)
Supported standards n/a
Measurement types Layer boundaries can be specified using automatic detection, magic wand or manual mode.
Individual measurements can be added or deleted later on.
  Measurement of any type of layers (with even or uneven boundaries) is supported.
Layer thickness measurement calculates mean, maximum and minimum values as well as statistical data for each individual layer.
Output Spreadsheet with individual measurements. Report automatically created in Microsoft Word.
Cast Iron Analysis
Description Measures the distribution, shape and size of graphite content in cast iron samples
Supported standards EN ISO 945-1, ASMT A247, JIS G5502, KS D 4302, GB/T 9441
Measurement types On polished samples: Automatic measures the characteristics of the graphite content (Size, Shape and Distribution)
On etched samples: Measures the ferrite to pearlite ratio
  Integrated workflow which take into account the sample status (etched or polished)
Stores all results in the image property for additional data mining.
Output Spreadsheet with individual measurements. Report automatically created in Microsoft Word.
Grain Sizing Intercept
Description Measures the mean grain index using the intercept method
Supported standards ASTM E112, GB/T 6394, GOST 5639, ISO 643, DIN 50601, JIS G0551, JIS G0552
Measurement types Selection of pattern (Circles, Cross, Cross & Circles, Vertical Lines, Horizontal Lines, Horizontal & Vertical Lines).
Definition of the number of test lines for determination of grain elongation.
  Display the g-value in the Material Solution Tool Window.
Stores all results in the image property for additional data mining.
Output Spreadsheet with individual measurements. Report automatically created in Microsoft Word.
Grain Sizing Planimetric
Description Measures the distribution of grain index using the planimetric method
Supported standards ASTM E112, GB/T 6394, GOST 5639, ISO 643, DIN 50601, JIS G0551, JIS G0552
Measurement types Automatic extraction of grain boundaries.
User interaction using Stream sliders for improved usability.
  Display the g-value histogram in the Material Solution Tool Window for direct interaction
Stores all results in the image property for additional data mining.
Output Spreadsheet with individual bins measurements, Stream chart with the distribution. Report automatically created in Microsoft Word.
Non Metallic Inclusion Rating in Steel
Description Measures the non metallic inclusion rating in steel using the worst field method
Supported standards ASTM E45 Method A, DIN 50602 Method M, ISO 4967 Method A, GB/T 10561 Method A, JIS G0555 Method A, UNI 3244 Method M
Measurement types Automatic detection of non metallic inclusion using colors, shape and size.
Automatic classification of oxydes, sulfides, silicates and aluminates
  Live display of the detected inclusion with its rating.
Stores all results in the image property for additional data mining.
Output Spreadsheet with individual measurements. Report automatically created in Microsoft Word.
Chart Comparison
Description Allows the user to compare live or selected images with standard charts
Supported standards ASTM E112, ISO 643 (Option), DIN 50602 (Option), ISO 945 (Option) and DIN 50601 (Option)
Measurement types Multiple displays available, including live overlay.
User interaction using Stream sliders for improved usability.
  Calculates statistics on the selected values.
Stores all results in the image property for additional data mining.
Output Report automatically created in Microsoft Word using selection of pre-defined templates.

 

Other Optional Software
Particle Detection
Description Automatically Count & Measure of objects and particles
Supported standards n/a
Measurement types Multiple threshold methods are available (automatic, manual HSV, manual and adaptative).
  The system can automatically measure multiple parameters on all segmentd objects (Area, Aspect Ratio, Bisector, Bounding box, Gravity Center, ID, Mass Center, Intensities Values, Convexity, Diameters, Elongation, Feret, Extent, Next Neighbor Distance, Orientation, Perimeter, Radius, Shape, Sphericity …).
Output Spreadsheet and charts with individual measurements. Report automatically created in Microsoft Word.
3D Surface View
Description Displays a 3D perspective of an image stack acquired with a motorised Z. (available with Stream Motion)
Display Creates a new image with a 3D perspective.
Easy navigation and rendering with the mouse
  Measurement of height profiles on the 2D projection
Output The result image can be exported. Animation can also be created automatically.
Netcam
Description Live transmission of images over the network
Display Remote Live view of the image using Microsoft Internet Explorer
Output Several users can share the same image at the same time.

 

PC Requirements
CPU Intel Core series 1.8 GHz or higher (Intel Core i5-650, 3.2 GHz recommended)
RAM 2GB or more recommended (4GB for Windows 7 64bit)
Hard disk 2 GB or more free space
OS Windows XP Professional SP3 (32 bit) / Windows Vista Business, Ultimate SP2 (32 bit) / Windows 7 Professional (32 bit/64 bit)
Graphic card 1280×1024 monitor resolution with 32 bit video card

1 kb Plus DNA Ladder，用于安全染料凝胶 #N0559S 125-250 gel lanes-NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品资料 – Markers 和 Ladders (DNA/RNA 和蛋白质) – DNA Ladders

1 kb Plus DNA Ladder，用于安全染料凝胶                              收藏

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价 格(元)
北京库存
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#N0559S
125-250 gel lanes
959.00
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特性

  • 针对 GelRed®，GelGreen® 和 SYBR™ Safe™ 琼脂糖预制凝胶进行了可视化优化（有关详细信息，请参阅下面的产品说明）
  • 使用方便，可直接上样
  • 片段范围：100 bp 至10 kb（100 bp 和 1 kb的 DNA Ladder 合二为一！）
  • 条带清晰易识别
  • 可上样 125 至 250 次

概述

 1 kb Plus DNA Ladder，用于安全染料凝胶，是一种预混的并可直接上样的分子量标准品，含有一种肉眼可见示踪染料。它已针对 GelRed®，GelGreen® 和 SYBR™ Safe™ 琼脂糖预制凝胶进行了优化。


1 kb Plus DNA Ladder，用于安全染料凝胶，由专有质粒经限制性内切酶消化后产生的 19 条带组成,适用于琼脂糖凝胶电泳分子量的标准条带。消化的 DNA 片段范围从 100 bp 至 10 kb。其中 0.5、1.0 和3.0 kb 条带为增加亮度的参考条带。
推荐凝胶浓度：1-1.4％
胶浓度为 1.2％ 时分离效果最佳
 
1 kb Plus DNA Ladder，用于安全染料凝胶 #N0559S 125-250 gel lanes
1 kb Plus DNA Ladder，用于安全染料凝胶，在1.0％ TBE 琼脂糖 GelRed 预制胶中的电泳条带。上样量为 0.5 μg/泳道。建议上样体积为：5 至 10 μl/泳道。
 
产品类别:
DNA Markers & Ladders 产品
应用: 
DNA 检测

条带碱基数量
1 kb Plus DNA Ladder，用于安全染料凝胶 #N0559S 125-250 gel lanes
 
有效检测范围
100 bp 至 10002 bp
储存温度
4℃
储存条件
5 mM Tris-HCl 
5 mM EDTA 
5% Glycerol 
0.002% Xylene Cyanol 
pH 8.0 @ 25℃

8ml 本色 HDPE广口试剂瓶

发表于由

8ml 本色 HDPE广口试剂瓶

货号：BS-RB-HDPE-0008-C

规格：20个/包

品牌：biosharp

商品详情：

本色 高密度聚乙烯（HDPE）试剂瓶

 

– 高密度聚乙烯，化学耐受性强，坚固耐用

– 颜色：本色，方便观察试剂状态

– 耐受温度范围-80℃至121℃

– 辐照灭菌

– 无内衬，螺纹处有专业防渗漏设计，高效防漏。

– 广口设计，溶液倾倒方便

货号 BS-RB-HDPE-0008-C
规格 20个/包
品牌 biosharp

6-keto-PGF1α ELISA kit——ENZO热销产品

发表于由

6-keto-PGF1α ELISA kit——ENZO热销产品

高灵敏ELISA试剂盒，3小时内可检测低至1.40 pg/ml 6-酮前列腺素F1α

 

6-酮-前列环素F1α（6-keto-PGF1α）是前列环素（PGI2）的稳定水解产物。由于前列环素在缓冲液中的半衰期很短（2-3分钟），通常通过测量尿液中的6-酮-前列环素F1α代谢物及其衍生物2,3-二酮-前列环素F1α来监测前列环素的产生。前列环素具有自分泌功能，包括抑制血小板聚集、抑制加压激素导致的血管收缩反应、血小板解聚等特性以及生殖功能。这些效应是由受体介导的cAMP的升高所引起的。前列腺素在动脉粥样硬化和保护心肌缺血方面有临床应用。

Enzo Life Sciences的6-酮-前列环素F1α比色法ELISA试剂盒，可在3小时内得到检测结果，在405nm处读取OD值。该试剂盒特异性高，与结构相关的分子交叉反应性低。6-酮-前列环素F1α ELISA试剂盒已经在各种类型样品中得到了充分验证。上海金畔生物作为Enzo life sciences中国代理，可提供该产品，欢迎咨询。

 

产品特点

◆ 三小时内可快速得到38份样品（带复孔）的实验结果

◆ 高特异性，与结构相关分子交叉反应性低

◆ 已使用各种样品进行了充分验证，可靠性高

◆ 广泛的文献引用

 

产品信息

产品货号

ADI-900-004/ ADI-901-004

产品名称

6-keto-PGF1α ELISA kit（6-酮前列腺素F1α ELISA试剂盒）

规格

96 wells/ 5×96 wells

其他名称

6-keto Prostaglandin F1α

灵敏度

1.40 pg/ml

检测范围

3.2 – 50,000 pg/ml

应用

ELISA, Colorimetric detection

适用样本类型

细胞培养上清，血清，唾液和尿液

检测波长

405 nm

适用种属

不限种属

运输

Blue Ice Not Frozen

长期保存

+4°C

试剂盒组分

DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, 

Standard, pNpp Substrate, Stop solution

 

标准曲线示例

6-keto-PGF1α ELISA kit——ENZO热销产品 

 

部分产品引用文献

1. Laser Speckle Contrast Imaging-based diagnosis of severe mesenteric traction syndrome: Hemodynamics and prostacyclin – A prospective cohort study: A.A. Olsen, et al.; Microvasc. Res. 147, 104505 (2023)

2. Contribution of cyclooxygenase-1-dependent prostacyclin synthesis to bradykinin-induced dermal extravasation: M. Krybus, et al.; Biomed. Pharmacother. 148, 112786 (2022)

3. Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage: K.M. Mirabito Colafella, et al.; Clin. Sci. (Lond.) 136, 675 (2022)

4. Estrogen modulates metabolic risk profile after resistance training in early postmenopausal women: a randomized controlled trial: T.V. Dam, et al.; Menopause 28, 1214 (2021)

5. Aspirin blocks formation of metastatic intravascular niches by inhibiting platelet-derived COX-1/thromboxane A2: S. Lucotti, et al.; J. Clin. Invest. 129, 1845 (2019)

6. Evidence for increased content of PGF2α, PGE2 and 6-keto-PGF1α in endometrial tissue cultures from heavy draft mares in anestrus with endometritis: M.J. Siemieniuch, et al.; J. Equine Vet. Sci. 77, 107 (2019)

7. Alterations in NO- and PGI2- dependent function in aorta in the orthotopic murine model of metastatic 4T1 breast cancer: relationship with pulmonary endothelial dysfunction and systemic inflammation: E. Buczek, et al.; BMC Cancer 18, 582 (2018)

8. Inhibitory Effects of an Orally Active Thromboxane A2 Receptor Antagonist, nstpbp5185, on Atherosclerosis in ApoE-Deficient Mice: S.W. Huang, et al.; Thromb. Haemost. 118, 401 (2018)

9. Advanced age in mares affects endometrial secretion of arachidonic acid metabolites during equine subclinical endometritis: M.J. Siemieniuch, et al.; Theriogenology 103, 191 (2017), Application(s): ELISA using culture supernatants

10. Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol. 89, 19 (2017), Application(s): Determine the concentrations of prostanoids in conditioned medium from myofibroblasts and HUVECs, and in HUVEC lysates

 

 

详情请咨询 ENZO 代理商-上海金畔生物科技

盐酸万古霉素

发表于由

盐酸万古霉素

货号：BS121-1g

规格：1g

品牌：biosharp

产品简介：
盐酸万古霉素是由东方链霉菌在控制条件下产生的一种两性糖肽类抗生素。它是一种窄
普抗生素，仅对革兰氏阳性菌有效。其作用机制是抑制细菌细胞壁的合成，通过与细菌细胞
壁结合，而使某些氨基酸不能进入细胞壁的糖肽中。在临床中，用于耐青霉素金葡菌引起的
严重感染，如肺炎、心内膜炎和败血症等，同时也对溶血性链球菌引起的感染及败血症起作
用。
英文名称：Vancomycin hydrochloride
别名：万古霉素盐酸盐；
CAS：1404-93-9
化学式：C66H75Cl2N9O24 · HCl
分子量：1485.73
含量：≥93%
PH：2.5~4.5
溶解度：50 mg/ml H2O
储存条件：2-8°C
外观（性状）：白色或类白色结晶粉末
单位：瓶
有效期：2 年
应用：1、用于革兰氏阳性菌感染，特别是对其它抗菌药耐药或疗效差的耐甲氧西林金黄色葡萄球
菌、表皮葡萄球菌、肠球菌所致严重感染。
2、用于治疗血液透析患者发生葡萄球菌属所致动、静脉分流感染。
3、口服适用于治疗假膜性结肠炎经甲硝唑治疗无效者，或多重耐药葡萄球菌小肠结肠炎。
使用方法：
根据实际需要参阅相关文献配制和使用。
注意：
1、本产品仅供科研使用，请勿用于医药、临床诊断或治疗，食品及化妆品等用途
2、为了您的安全和健康，请穿实验服并戴一次性手套操作
货号 BS121-1g
规格 1g
品牌 biosharp
说明书下载 点击下载

eppendorf 艾本德5810 / 5810 R台式高速大容量离心机

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eppendorf 艾本德5810 / 5810 R台式高速大容量离心机

eppendorf 艾本德5810 / 5810 R台式高速大容量离心机

产品编号：
市场价：￥0.00元
会员价：￥0.00元
品牌：EPPENDORF 艾本德
生产厂家：eppendorf 艾本德

  • 产品概览
  • 技术参数
  • 订购信息
  • 相关资料
  • 相关产品

5810 / 5810 R台式高速大容量离心机

历史悠久的Eppendorf台式离心机在过去的每一年中被广泛使用。因而，Eppendorf非常自然将开发的离心机新技术应用于活细胞处理。因而5804/5804R和5810/5810R虽是一款具备同样底盘的设备，但却拥有改良的控制面板和融汇先进工艺的内在 生命力！

在详细陈述优异的特性和创新技术之前，我们首先向那些使用过5804/5810的用户着重推荐新一代改良机型。

优化的操作界面：

新的彩色控制面板使参数输入更为简化，包括触觉和声音两方面。一个单独的short-spin键可以快速完成瞬时离心功能。对于新的冷冻离心机，Stand-by键可以使离心机待机时明显节约用电。在这个系列离心机中，新增设了at set rpm定速记时功能，当离心机达到设定速度时，方才开始倒数计时，从而提高了不同离心机严格按照操作规程的可比性。离心结束时的提示音可以根据客户的需要而予以消除。

最短的刹车时间：

我们再次通过优化发动机来减少离心机启动和刹车时间，尤其对于一些较大的水平转子来说，时间可以减少一半，从而使所有转子的刹车时间都低于1分钟。

制冷技术：

特别的成就在于使离心能力在不同的电压下有较大提高。在台式离心机面世以来，转子的设置一直作为关键因素来发展，几乎所有的转子均可以达到4°C，并且在正常室温条件下和最高转速时保持此温度。持有专利的压缩机中频率调制器大大降低了仪器运行时产生的振动：既有利于离心样品本身，又降低噪音的产生。气流调节器也是如此。

Eppendorf新推出的5804台式多功能高速离心机（冷冻型：5804R）和5810台式高速大容量离心机（冷冻型:5810R），为离心机的灵活性和处理能力建立了新标准。全面广泛的标准配件系统涵盖了PCR管套，1.5/2 ml标准管以及大容量的Falcon®管、Falcon®瓶和滤板系统，因而实际上适用于所有高达400 ml容量的冷冻和非冷冻型离心。

这两个型号的处理能力虽然不同，但在使用者针对个人要求进行选型后，两个型号的操作却是相同的。

使用水平转子的5804离心机处理能力可达400 ml,而使用水平转子的5810离心机处理能力可高达1,600 ml。通过选择不同 种类的转子，可以灵活使用一系列各种容量和形状的管子。

除了以往适用于多种通用管、微孔板和深孔板的水平转子和固定角转外，现在又推出两款新型转子：PCR管套型转子和水平转子A-4-81，后者在5810/5810R型离心机上使用，可放置多达20 x 50 ml Falcon®管。标准组合式适配器使转子更加适合用户的各种需要。

型号 5810 型离心机
尺寸(长x 宽x 高) cm： 54 x 61 x 35
重量(kg)： 68
最大功率要求： 900 W
电源： 所有型号均为230 V/50-60 Hz