天然免疫学热点领域科研抗体(AbD Serotec®)—报告蛋白抗体

发表于2025年1月9日由上海金畔生物科技有限公司

报告蛋白通过酶催化或荧光直读的方式来监测细胞转染、蛋白表达模式以及启动子功能。这些报告蛋白可以直接检测，或者像下图利用报告蛋白特异性抗体对报告蛋白进行western blot检测。

使用mouse anti-GST(MCA1352)和anti-mouse IgG-HRP(STAR13B )对GST-ubiquitin 进行Western blot检测

AbD Serotec公司最受欢迎的的报告蛋白抗体

产品名称	克隆号	形式	应用	产品编号
B- Galactosidase	4722 HuCAL	Purified	E	HCA033
	4723 HuCAL	Purified	E	HCA034
	4724 HuCAL	Purified	E	HCA035
Green Fluorescent Protein (GFP)	264-449-2	Purified	C, IB, IP	OBT1643
	338 HuCAL	Purified	E	HCA060
	N/K	Purified	C, E, P, WB	OBT1820
	Poly	Purified	E, IF	4745-1051
	Poly	Purified	E, IF, WB	AHP975
	Poly	Purified	E, IB	OBT1645
Glutathione-S- Transferase (GST)	2021 HuCAL	Purified	E, WB	HCA055
	2031 HuCAL	Purified, FITC, HRP	E, WB	HCA056
	3937 HuCAL	Purified	E, WB	HCA054
	Vpg66	S/N,Biotin	E, IF, WB	MCA1352
Luciferase	BGN/06/942	Purified	E	5709-4360
	BGN/06/944	Purified	E	5709-4380
	LUC-Y	Purified	C, E, F, WB	MCA2076

Bevacizumab antibody | AbD17976_hIgG1

发表于2024年12月28日由上海金畔生物科技有限公司

Bevacizumab antibody | AbD17976_hIgG1

人Anti-Bevacizumab Antibody, clone AbD17976_hIgG1是重组抑制性抗独特型IgG抗体，可特异性的识别人单克隆抗体药bevacizumab, ranibizumab及其他生物类似物，可在Anti-Drug Antibody (ADA) Bridging ELISA中用作阳性对照或者校准品。

人Anti-Bevacizumab Antibody, clone AbD17976_hIgG1与抗体药ranibizumab (Lucentis)有交叉反应。Ranibizumab是一种人源化Fab抗体（VH3-23/kappa1），来源于与bevacizumab相同的亲代小鼠。

Bevacizumab (商品名称为Avastin)是人源的单克隆抗体(IgG1/kappa)，抑制通过结合血管内皮生长因子α（VEGF-A）抑制血管生成。VEGF-A是正常细胞和癌细胞血管生成的刺激因子，是血管生成的调节因子。Bevacizumab用于治疗多种癌症，包括结直肠癌、肺癌、乳腺癌、胶质母细胞瘤、肾癌和卵巢癌。

产品信息

货号HCA185 HCA185G

包装规格0.1 mg 1mg

类型单克隆抗体

克隆号AbD17976_hIgG1

亚型IgG1

免疫原Bevacizumab

形式液体，人源细胞表达

来源Protein A亲和层析

缓冲液磷酸盐

防腐剂0.01%硫柳汞

浓度Pack Size: 0.1 mg 抗体浓度1.0 mg/ml

Pack Size: 1 mg 总蛋白浓度1.0 mg/ml

保存条件

室温运输，建议到货后分装保存在-20°C

解冻后可在2-8°C最长保存4周，其余保存在-20°C

不建议储存在无霜冰箱

避免反复冻融，以免抗体变性

应用

ELISA

可在Anti-Drug Antibody (ADA) Bridging ELISA中用作参考物

更多详情请咨询Bio-Rad优势代理商-上海金畔生物

Denosumab antibody | AbD26296

发表于2024年12月26日由上海金畔生物科技有限公司

Denosumab antibody | AbD26296

Human Anti-Denosumab Antibody, clone AbD26296 是一个抗原决定簇特异的，专门识别单抗药物denosumab的抗独特型抗体。这个抗体不识别free RANKL (receptor activator of nuclear factor kappa-B ligand)或与人RANKL结合的denosumab。该抗体用于检测free denosumab及相似的产品，推荐在pharmacokinetic (PK) bridging ELISA中用于捕获抗体，HCA280作为检测抗体。

Denosumab (Prolia, Xgeva)是一种人单克隆抗体 (IgG2/kappa)，用于治疗骨质疏松症、治疗性骨质流失、骨转移、多发性骨髓瘤和骨巨细胞瘤。该药物与人RANKL特异性结合，该蛋白质作为主要信号促进骨去除/吸收并防止RANKL激活破骨细胞及其前体表面上的受体RANK。

产品信息

货号HCA288

包装规格0.1 mg

类型单克隆抗体

克隆号AbD26296

亚型HuCAL单价Fab

免疫原Denosumab

形式液体， E. coli中表达，携带一个 DYKDDDDK 标签和一个HIS-tag (HHHHHH)标签

来源金属螯合亲和层析

缓冲液磷酸盐

防腐剂0.01%硫柳汞

抗体浓度0.5mg/ml

保存条件

室温运输，建议到货后分装保存在-20°C

解冻后可在2-8°C最长保存4周，其余保存在-20°C

不建议储存在无霜冰箱

避免反复冻融，以免抗体变性

应用

ELISA

更多详情请咨询Bio-Rad优势代理商-上海金畔生物

犬CD分子抗体 Abd serotec公司

发表于2024年12月25日由上海金畔生物科技有限公司

犬CD分子抗体 Abd serotec公司

狗CD标志物抗体

AbD Serotec 可提供多种针对狗CD标志物抗原的抗体。同时还提供即用型双染或三染流式抗体试剂盒，使用更方便。

Dog CD Marker Antibodies

Specificity	Clone	Applications	Product Code	Format	T cell	B cell	Dendritic cell	NK cell	Monocyte/macrophage
CD3	CA17.2A12	C, F, IF, IP, P	MCA1774GA	Purified, FITC	✓
CD3/CD4	CA17.2A12, YKIX302.9	F	DC046	FITC/RPE	✓
CD3/CD8	CA17.2A12, YCATE55.9	F	DC047	FITC/RPE	✓
CD3/CD4/CD8	CA17.2A12, YKIX302.9, YCATE55.9	F	TC014	FITC/RPE/Alexa, Fluor® 647	✓
CD4	YKIX302.9	C, F	MCA1038GA	Purified, Alexa Fluor 488, Alexa Fluor 647, APC, FITC, Pacific Blue™, RPE	✓
CD4	CA13.1E4	C, F, IP	MCA1998S	S/N	✓
CD4/CD8	YKIX302.9,YCATE55.9	F	DC048	FITC/RPE	✓
CD5	YKIX322.3	C, F, IP	MCA1037GA	Purified, Alexa Fluor488, Alexa Fluor647, APC, FITC, RPE	✓			✓
CD8	YCATE55.9	C, F, IF	MCA1039GA	Purified, Alexa Fluor647, Alexa Fluor700, FITC, Pacific Blue, RPE	✓
CD8 Alpha	CA9.JD3	C, F, IP	MCA1999S	S/N	✓
CD8 Beta	CA15.4G2	C, F, IP	MCA1775S	S/N	✓
CD11/CD18	YKIX490.6.4	F, IP	MCA1040G	Purified, FITC	✓	✓	✓	✓	✓
CD11b	CA16.3E10	C, F, IP	MCA1777S	S/N				✓	✓
CD11c	CA11.6A1	C, F, IP	MCA1778S	S/N			✓		✓
CD11d	CA11.8H2	C, F, IP	MCA1779GA	Purified, Alexa Fluor 647, S/N	✓				✓
CD11d	CA16.3D3	C, F	MCA2039S	S/N
CD18	CA1.4E9	C, F, IF, IP	MCA1780GA	Purified, Alexa Fluor 647	✓	✓	✓	✓	✓
CD21	CA2.1D6	C, F, IP	MCA1781R	Purified, Alexa Fluor 647, RPE		✓

Dog CD Marker Antibodies

Specificity	Clone	Applications	Product Code	Format	T cell	B cell	Dendritic cell	NK cell	Monocyte/macrophage
CD25	P4A10	F, WB	MCA5916GA	Purified, FITC, RPE	✓
CD34	1H6	F, WB	MCA2411GA	Purified, Alexa, Fluor 647, Biotin, FITC, RPE
CD44	YKIX337.8.7	C, F	MCA1041GA	Purified, Alexa, Fluor 488	✓	✓			✓
CD45	YKIX716.13	F, IP	MCA1042GA	Purified, Alexa, Fluor 488, Alexa, Fluor 647, APC, Biotin, FITC, RPE	✓	✓			✓
CD45	CA12.10C12	C, F, P	MCA2035S	S/N	✓	✓			✓
CD45R	YKIX753.22.2	C, CT, F, IP	MCA1043G	Purified, Alexa, Fluor 488, Alexa, Fluor 647, FITC, RPE	✓	✓			✓
CD45RA	CA4.1D3	C, F	MCA2036S	S/N	✓	✓			✓
CD90	YKIX337.217	F, IP	MCA1036G	Purified	✓				✓
CD107b	AC17	F, IF, IP, WB	MCA2558	Purified, Alexa, Fluor 647, FITC, RPE					✓

Applications: C = Immunohistology – Frozen, CT= Cytotoxic Assays, E = ELISA, F = Flow Cytometry, FN= Functional Assays,
IF= Immunofluorescence, IP = Immunoprecipitation, P = Immunohistology – Paraffin, WB = Western Blotting.

*信息来自内部实验室测试、同行评审刊物或研究人员的个人交流。

Bio-Rad（Abd serotec）–独特性抗体

发表于2024年11月12日由上海金畔生物科技有限公司

Bio-Rad（Abd serotec）–独特性抗体

当一种抗体与另一种抗体的独特型结合时，它被称为抗独特型抗体。抗体的可变部分包括独特的抗原结合位点，称为独特型。独特型(即独特型)内表位的组合对于每种抗体都是独特的。

Bio-Rad（Abd serotec）--独特性抗体

如今开发的大多数治疗性单克隆抗体是人的或人源化的，用于诱导抗药物抗体(ADA)的最可能免疫原性表位位于提供大多数结合接触的高变互补决定区(CDR)内。可以产生抗独特型抗体来特异性结合一种单克隆抗体药物。这些高度专业化的抗体可用于建立不同形式的药代动力学(PK)测定，以测量临床前和临床样品中的游离或总药物水平，或作为ADA测定中的阳性对照。

对于使用HuCAL技术产生抗独特型抗体，在存在作为阻断剂的同种型亚类匹配抗体的情况下对药物进行抗体选择，以避免与抗体药物的其他区域结合的特异性富集，并确保独特型特异性。此外，在人血清存在下进行的选择避免了最终分析中的基质效应。

Bio-rad可以指导选择方法产生具有不同结合模式和性质的抗独特型抗体，如图2所示。中和抗体(type1)是基于细胞的检测和LISA的理想选择。选择与药物抗原结合位点外的独特体结合的抗体，产生可用于检测样品中游离药物和结合药物的抗体(type2)bio-rad的独特之处在于分离罕见特异性的能力，例如可用于定量结合药物的药物-靶标复合结合物(type 3)，而不是游离药物水平。

（图2）

以下是bio-rad可以提供抗独特型抗体

Anti-Adalimumab Antibodies

货号	Antibody specificity	Binding Type	Format	Assay Development Recommendations
HCA202	Adalimumab	Type 1	Fab-FH	Direct and indirect ELISAPK bridging ELISA with HCA204 Inhibition ELISA
HCA203	Adalimumab	Type 1	Human IgG1	Direct ELISAADAassay Inhibition ELISA
HCA204	Adalimumab	Type 1	Human IgG1	PK bridging ELISA with HCA202 ADAassay Inhibition ELISA
HCA204P	Adalimumab	Type 1	Human IgG1 HRP labeled	PK bridging ELISA with HCA202 Inhibition ELISA
HCA271	Adalimumab	Type 1	Human IgE	ADAassay for IgE
HCA206	Adalimumab/TNF-α	Type 3	Fab-FH	PK antigen capture format
HCA207	Adalimumab/TNF-α	Type 3	Human IgG1	PK antigen capture format
HCA231	Adalimumab/TNF-α	Type 3	Fab-FH	PK antigen capture format
HCA232	Adalimumab/TNF-α	Type 3	Human IgG1	PK antigen capture format
HCA232P	Adalimumab/TNF-α	Type 3	Human IgG1 HRP labeled	PK antigen capture format

Anti-Adalimumab Antibodies

货号	Antibody specificity	Format	Assay Development Recommendations
HCA174	Alemtuzumab	Fab-V5Sx2	PK bridging ELISAcapture antibody
HCA175	Alemtuzumab	Human IgG1	PK bridging ELISAdetection antibody ADAassay
HCA175P	Alemtuzumab	Human IgG1 HRP labeled	PK bridging ELISAdetection antibody with HCA198
HCA198	Alemtuzumab	Fab-FH	PK bridging ELISAcapture antibody with HCA175P
HCA199	Alemtuzumab	Human IgG1	PK bridging ELISAdetection antibody ADAassay

Anti-Bevacizumab Antibodies

货号	Antibody specificity	Binding Type	Format	Assay Development Recommendations
HCA182	Bevacizumab	Type 1	Fab- V5Sx2	Indirect ELISAPK bridging ELISAwith HCA184P
HCA184	Bevacizumab	Type 1	Human IgG1	Indirect ELISAPK bridging ELISAwith HCA182
HCA184P	Bevacizumab	Type 1	Human IgG1 HRP labeled	Direct ELISAPK bridging ELISAwith HCA182
HCA185	Bevacizumab	Type 1	Human IgG1	Indirect ELISAADAassay

除以上种类以外，还有如下⼀些单抗的独特型抗体，详情请咨询金畔

Ceruximab Erbitux	Denosumab Prolia	Eculizumab Soliris
Etanercept Enbrel	Golimumab Simponi	Infliximab Remicade
Natalizumab Tysabri	Nivolumab Opdivo	Omalizumab Xolair
Palivizumab Synagis	Panitumumab Vectibix	Pembrolizumab Keytruda
Ranibizumab Lucentis	Rituximab Rituxan	Tocilizumab Actemra
Trastuzumab Herceptin	Ustekinumab ATELARA	Vedolizumab Entyvio

更多详情，请咨询Bio Rad国内代理商-上海金畔生物

天然免疫学热点领域科研抗体(AbD Serotec®)—巨噬细胞标志物抗体

发表于2024年10月17日由上海金畔生物科技有限公司

天然免疫学热点领域科研抗体(AbD Serotec®)—巨噬细胞标志物抗体

AbD Serotec公司是全球领先的巨噬细胞标志物抗体供应商，其生产的MCA497, Rat Anti Mouse F4/80 Clone CI:A3-1是著名的成熟小鼠巨噬细胞表面特异性标志物抗体，有将近400篇的文献引用，提供多达30种的包装形式，可用于FC、WB，IF、IHC等7种应用。AbD Serotec享有该克隆的专利，是该抗体唯一的商业化生产商。
MCA1957识别小鼠细胞表面macrosialin，为人CD68的同源物，是专门表达于组织巨噬细胞的一种重度糖基化的跨膜糖蛋白。

小鼠淋巴结的免疫荧光染色；巨噬细胞用anti 小鼠脾脏冰冻切片的免疫组化染色；红髓中的
F4/80(MCA497PE)染成红色，B细胞用anti C- 巨噬细胞用anti CD68(MCA1957)染色
D79b(MCA1821A488)染成绿色，细胞核用D-
API复染，呈蓝色

AbD Serotec公司提供的最受欢迎的巨噬细胞标志物抗体

人巨噬细胞标志物抗体
产品名称	克隆号		产品编号	形式
CD11b	ICRF44		MCA551	Pur., A647, A700, Az. Fr., Biot., FITC, RPE, RPE-A647
CD14	UCHM1		MCA596	Pur., A647, APC, Az. Fr., Biot., FITC, RPE
CD14	TUK4		MCA1568	Pur., A647, A700, Biot., FITC, LE, PB, RPE, RPE-A647, RPE-A750
CD14	MEM-18		MCA2185	Pur., FITC, RPE
CD14	61D3		MCA2804	RPE-Cy5, RPE-Cy5.5
CD163	EDHu-1		MCA1853	Pur., FITC
CD169	7-239		MCA2517	Pur., A488, A647, FITC, LE, RPE
CD200R	OX108		MCA2282	Pur., A488, A647, Biot., FITC, RPE
CD204	Poly		AHP563	Serum
CD206	15-2		MCA2155	Pur.
CD209	MR-1		MCA2318	Pur., A488, A647, Biot., FITC, LE, RPE
CD31	WM59		MCA1738	Pur., A647, Biot., FITC, RPE
CD36	SMØ		MCA722	Pur., A647, Az. Fr., FITC
CD68	514H12		MCA1815	S/N
CD68	KiM7		MCA2375F	FITC
Dectin-1	BD6		MCA4662	Pur., A488, A647, FITC, RPE
Dectin-1	GE2		MCA4661	Pur., A488, A647, FITC, LE, RPE
Macrophage	MAC387		MCA874	Pur., A488, FITC, LE, RPE
小鼠巨噬细胞标志物抗体
CD11b		M1/70.15	MCA74	Pur., A488, A647, A700, Biot., FITC, LE, RPE, RPE- A647, RPE-A750, RPE-Cy5
CD11b		5C6	MCA711	Pur., FITC, LE, RPE
CD115		604B5	2E11	MCA1898 Pur., Az. Fr., RPE
CD169		MOMA-1	MCA947	Pur., S/N, A647, FITC
CD169		3D6.112	MCA884	Pur., FITC, LE
CD200R		OX-110	MCA2281	Pur., A488, A647, Biot., FITC, LE, RPE
CD204		2F8	MCA1322	Pur., A488, A647, Biot., FITC, LE, RPE
CD206		MR5D3	MCA2235	Pur., A488, A647, Biot., FITC, LE, RPE
CD31		ER-MP12	MCA2388	Pur., A488, A647, Biot., RPE, RPE-A647
CD36		MF3	MCA2748	Pur., A488, A647, FITC, LE, RPE
CD68		FA-11	MCA1957	Pur., A488, A647, A700, Az. Fr., Biot., FITC, RPE
Dectin-1		2A11	MCA2289	Pur., A647, Biot., FITC, LE, RPE
Dectin-2		D2.11E4	MCA2415	Pur., A488, A647, Biot., FITC, LE, RPE
F4/80		CI:A3-1	MCA497	Pur., A488, A647, A700, APC, Biot., FITC, LE, PB, RPE, RPE-A647, RPE-A750, S/N
Ly-6B.2		7/4	MCA771	Pur., A647, A700, Biot., FITC, LE, RPE
Ly-6C		ER-MP20	MCA2389	Pur., A488, A647, Biot., RPE
MARCO		ED31	MCA1849	Pur., FITC, RPE
TREM-1		L5-B8.2A12.3A12	MCA2578	Pur., A488, A647, FITC, LE, RPE
TREM-2		78.18	MCA4772	Pur., FITC, LE
大鼠巨噬细胞标志物抗体
CD11b		OX-42	MCA275	Pur., A488, A647, A700, Biot., FITC, LE, PB, RPE, RPE- A647
CD11b		ED7	MCA618R	Pur.
CD11b		ED8	MCA619	Pur., FITC, RPE
CD115		604B52E11	MCA1898	Pur., Az. Fr., RPE
CD163		ED2	MCA342	Pur., A647, Biot., FITC, LE, RPE
CD169		ED3	MCA343	Pur.
CD172a		OX41	MCA274	Pur., Az. Fr., Biot, FITC, RPE
CD172a		ED9	MCA620	Pur., FITC, RPE
CD200R1		OX-102	MCA1959	Pur., Biot., FITC, LE, RPE
CD31		TLD-3A12	MCA1334	Pur., A488, A647, Biot., FITC, LE, RPE
CD68		ED1	MCA341	Pur., A488, A647, A700, Biot., FITC, RPE

荧光多色染色试剂

产品名称	克隆号	产品编号	形式
Human CD45/CD14	F10-89-4/UCHM1	DC008	FITC/RPE
Mouse IgG2a/IgG2a	Negative Control	DC013	FITC/RPE
Mouse Ly6C/CD31	ERMP20/ERMP12	DC053	A488/RPE
Mouse Ly6C/CD31	ERMP20/ERMP12	DC054	A488/RPE-A647
Mouse CD11b/CD115	M1/70.15 / 604B5 2E11	DC055	A488/RPE
Mouse Ly6C/CD115/CD11b	ERMP20/604B52E11/M1/70.15	TC015	A488/RPE/RPE-A647
Mouse Ly6C/CD115/CD31	ERMP20/604B52E11/ERMP12	TC016	A488/RPE/RPE-A647
Mouse Ly6C/CD115/CD31	ERMP20/604B52E11/ERMP12	TC017	A488/RPE/A647
Mouse Ly6C/CD115/CD11b/CD31	ERMP20/604B52E11/M1/70.15/ERMP12	QC001	A488/RPE/RPE-A647/A647

AbD Serotec公司 F4/80 产品参考文献
1. Austyn, J.M. and Gordon, S. (1981) F4/80 , a monoclonal antibody directed specifically against the mouse macrophage.Eur. J. Immunol. 11: 805-815.
2. Hume, D.A., Perry, V.H., and Gordon, S. (1984) The Mononuclear Phagocyte System of the Mouse defined by Immunohistochemical Localisation of Antigen F4/80: Macrophages Associated with Epithelia.The Anatomical Record 210: 503-512.
3. Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.J. Exp. Med. 161: 475-489.
4. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
Curr. Top. Micro. Immunol. 181: 1-37.
5. Uaesoontrachoon, K. et al. (2013) Osteopontin deficiency delays inflammatory infiltration and the onset of muscle regeneration in a mouse model of muscle injury.Dis Model Mech. 6: 197-205.
6. Lin, H. H.et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.J. Exp. Med. 201: 1615–1625.
7. Chan, R.J. et al. (2005) Human somatic PTPN11 mutations induce hematopoietic cell hypersensitivity to granulocyte-macrophage colony stimulating factor.Blood. 105: 3737-3742.
8. Pizza, F.X. et al. (2005) Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice.J. Physiol. 562:899-913.
9. Tarallo, V. et al. (2011) The biflavonoid amentoflavone inhibits neovascularization preventing the activity of proangiogenic vascular endothelial growth factors.J Biol Chem. 286: 19641-51.
10. Rivollier, A. et al. (2012) Inflammation switches the differentiation program of Ly6Chi monocytes from antiinflammatory macrophages to inflammatory dendritic cells in the colon.
J Exp Med. 209: 139-55.
11. Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member,Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linkedmarker for subsets of mouse macrophages and dendritic cells.J Immunol. 182:1278-86.
12. Seitz, O. et al. (2010) Wound Healing in Mice with High-Fat Diet- or ob Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study.Exp Diabetes Res. 2010: 476969.
13. Miao, E.A. et al. (2011) Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria.Nat Immunol. 11: 1136-42.
14. Wang, X. et al. (2011) Activation of the cholinergic antiinflammatory pathway ameliorates obesity-induced inflammation and insulin resistance.Endocrinology. 152: 836-46.
15. Cunningham, O. et al. (2009) Microglia and the urokinase plasminogen activator receptor/uPA system ininnate brain inflammation.Glia. 57: 1802-14.
16. Gornicka, A. et al. (2012) Adipocyte hypertrophy is associated with lysosomal permeability both in vivo and in vitro: role in adipose tissue inflammation.Am J Physiol Endocrinol Metab. 303: E597-606.
17. Akbarshahi, H. et al. (2012) Enrichment of Murine CD68(+)CCR2(+) and CD68(+)CD206(+) Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.PLoS One. 7: e42654.
18. Banda, N.K. et al. (1012) Role of C3a receptors, C5a receptors, and complement protein C6 deficiency in collagen antibody-induced arthritis in mice.J Immunol. 188: 1469-78.
19. Bonde, A.K. et al. (2012) Intratumoral macrophages contribute to epithelial-mesenchymal transition in solid tumors.BMC Cancer. 12: 35.
20. Choi, K.M. et al. (2010) CD206-positive M2 macrophages that express heme oxygenase-1 protect against diabetic gastroparesis in mice.Gastroenterology. 138: 2399-409,
21. Tamaki, S. et al. (2013) Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction.PLoS One. 8: e68893.
22. Kihira, Y. et al. (2014) Deletion of hypoxia-inducible factor-1a in adipocytes enhances glucagon-like Peptide-1 secretion and reduces adipose tissue inflammation.PLoS One. 9(4):e93856.

牛CD分子抗体 Abd serotec公司

发表于2024年10月11日由上海金畔生物科技有限公司

牛CD分子抗体 Abd serotec公司

牛CD标志物抗体

许多顶级刊物文章中经常引用的兽医免疫学试剂很多都来自AbD Serotec，AbD Serotec 可提供多种针对牛CD标志物的抗体。这些抗体主要是用来研究牛适应性和先天性免疫系统，可帮助阐释T细胞、B细胞、树突细胞和巨噬细胞的功能。同时，我们将继续开发基础免疫功能、诊断和疫苗开发试剂，并且保证所有的试剂都要通过验证合格才可出厂。

Cow CD Marker Antibodies

Specificity	Clone	Applications	Product Code	Format	T cell	B cell	Dendritic cell	NK cell	Monocyte/macrophage
CD1w2	CC13	F	MCA1646G	Purified			✓
CD1w2	CC20	C, F, IP	MCA2058G	Purified, RPE, S/N			✓
CD1w2	CC14	C, F, IP	MCA831G	Purified, Ascites			✓
CD1w3	CC43	F, IP	MCA1647G	Purified, S/N		✓	✓		✓
CD2	CC42	C, F, IP, P	MCA833G	Purified, Ascites, FITC	✓			✓
CD4	CC8	C, F, IP	MCA1653GA	Purified, Alexa, Fluor® 647, FITC, RPE	✓
CD4	CC30	C, F, IP, P	MCA834GA	Purified	✓
CD5	CC17	C, F, IP	MCA835G	Purified, Alexa, Fluor 647, FITC	✓	✓
CD6	CC38	C, F, IP	MCA836	Ascites	✓
CD8	CC63	C, F, IF, IP, P	MCA837GA	Purified, Alexa, Fluor 647, FITC, RPE		✓		✓
CD8beta	CC58	F, IP	MCA1654G	Purified, RPE		✓
CD11a	IL-A99	F, IP	MCA2429	Purified, FITC, RPE	✓	✓	✓	✓	✓
CD11b	CC126	C, F, IP	MCA1425GA	Purified, FITC				✓	✓
CD13	CC81	F	MCA2338	Purified, FITC			✓
CD14	CC-G33	F, IF	MCA2678GA	Purified, FITC					✓
CD21	CC21	C, F, IF, IP	MCA1424GA	Purified, FITC, RPE		✓
CD21	CC51	F	MCA5953GA	Purified, FITC, RPE		✓
CD25	IL-A111	C, F, IP	MCA2430GA	Purified, FITC, RPE	✓			✓
CD26	CC69	F, IP	MCA1652G	Purified, Alexa, Fluor 647, S/N		✓	✓	✓
CD28	CC219	F, FN	MCA5779	Purifi ed, FITC, EL, RPE	✓
CD28	CC220	F, FN	MCA5778	Purified, EL	✓
CD32	CCG36	C, F	MCA5918GA	Purified, FITC, RPE		✓	✓		✓

Cow CD Marker Antibodies

Specificity	Clone	Applications	Product Code	Format	T cell	B cell	Dendritic cell	NK cell	Monocyte/macrophage
CD32	CCG39	C, F	MCA5919GA	Purified, FITC		✓	✓		✓
CD40	IL-A156	F, FN	MCA2431GA	Purified, FITC, RPE		✓
CD41	IL-A164	C, F, IP	MCA2432	Purified, FITC, RPE
CD44	IL-A118	F	MCA2433	Purified, FITC, RPE	✓	✓			✓
CD45	CC1	C, F, IP	MCA832GA	Purified, FITC	✓	✓
CD45RB	CC76	C, F, IP	MCA1650G	Purified, FITC, S/N	✓
CD45RO	IL-A116	F, IP	MCA2434GA	Purified, RPE	✓				✓
CD62L	CC32	F	MCA1649G	Purified, FITC, S/N	✓	✓		✓	✓
CD63	CC25	F	MCA2042GA	Purified			✓		✓
CD80	IL-A159	F	MCA2436GA	Purified, FITC, RPE		✓	✓		✓
CD80	IL-A190	F	MCA2437GA	Purified, FITC, RPE		✓	✓		✓
CD172a	CC149	F	MCA2041GA	Purified, RPE-Cy5			✓		✓
CD205	CC98	C, F, IP	MCA1651GA	Purified, FITC			✓
CD205	IL-A114	C, F	MCA2450GA	Purified, FITC, RPE			✓
CD282	12542	F	HCA151	Purified, Alexa, Fluor 647, FITC			✓		✓
CD282	12538	F	HCA152	Purified, Alexa, Fluor 647, FITC			✓		✓
CD335	AKS1	F, FN, IF, WB	MCA2365GA	Purified, Alexa, Fluor 488, EL, RPE				✓

*信息来自内部实验室测试、同行评审刊物或研究人员的个人交流。

Tocilizumab antibody | AbD21338_hIgG1

发表于2024年10月10日由上海金畔生物科技有限公司

Tocilizumab antibody | AbD21338_hIgG1

Human Anti-Tocilizumab Antibody, clone AbD21338是重组的抗独特性抗体，可特异性识别游离的tocilizumab，不与人IL-6R或者药物IL-6R复合物反应，可用来检测tocilizumab及生物类似物的浓度。推荐在pharmacokinetic (PK) bridging ELISA用作检测抗体，或者在anti-drug antibody (ADA) bridging ELISA用作参考品。

Tocilizumab（RoActemra/Actemra）是一种人源化IgG1/kappa单克隆抗体，被批准用于治疗中重度类风湿性关节炎和全身性青少年特发性关节炎。治疗性抗体针对IL-6R，通过阻断IL-6R（可溶性形式sIL-6R和膜结合形式mIL-6R）与IL-6的结合发挥作用，从而阻止该细胞因子的促炎症特性。

产品信息

货号HCA253 HCA253G

包装规格0.1mg 1mg

类型单克隆抗体

克隆号AbD21338_hIgG1

亚型IgG1

免疫原Tocilizumab

形式液体，人源细胞表达

来源Protein A亲和层析

缓冲液磷酸盐

防腐剂0.01%硫柳汞

抗体浓度Pack Size: 0.1 mg 浓度0.5 mg/ml

Pack Size: 1 mg 浓度1.0 mg/ml

应用ELISA

保存条件

室温运输，建议到货后分装保存在-20°C

解冻后可在2-8°C最长保存4周，其余保存在-20°C

不建议储存在无霜冰箱

避免反复冻融，以免抗体变性

如需购买或者了解更多Tocilizumab antibody | AbD21338_hIgG1产品信息，请联系Bio-Rad中国代理商上海金畔生物。

天然免疫学热点领域科研抗体(AbD Serotec®)—树突状细胞标志物抗体

发表于2024年10月4日由上海金畔生物科技有限公司

天然免疫学热点领域科研抗体(AbD Serotec®)—树突状细胞标志物抗体

树突状细胞（Dendritic cells, DC）是机体功能最强的专职抗原递呈细胞(Antigen presenting cells, APC)，它能高效地摄取、加工处理和递呈抗原，在天然免疫和获得性免疫抗中发挥着重要的作用。

AbD Serotec公司精选的树突状细胞标志物抗体

人树突状细胞标志物抗体
产品名称	克隆号	产品编号	形式
CD1a	NA1/34-HLK	MCA80	Pur., A488, A647, Biot., FITC, RPE
CD1a	O10	MCA1657	Ready To Use
CD13	WM15	MCA1270	Pur., A488, A647, A700, APC, FITC, LE, RPE
CD40	LOB7/6	MCA1590	Pur., A488, A647, Biot., FITC, LE, RPE
CD43	DFT-1	MCA555	Pur., A647, Az. Fr., Biot., FITC, RPE
CD70	Bu69	MCA1765G	Pur.
CD80	MEM-233	MCA2071	Pur., A647, Az. Fr., Biot., FITC, RPE
CD83	HB15e	MCA1582	Pur., A488, A647, Biot., FITC, RPE, RPE-CY5
CD86	BU63	MCA1118	Pur., FITC, LE, RPE, RPE-CY5
CD91	A2Mr alpha-2	MCA1965	Pur., Az. Fr., Biot., FITC, RPE
CD103	LF61	MCA1416	Pur., FITC, LE, RPE, RPE-CY5
CD107b	Poly	AHP163	Pur.
CD123	6H6	MCA1263T	Pur., Az. Fr.
CD135	BV10A4H2	MCA1843	Biot.
CD141	QBEND/40	MCA641	S/N
CD184	Poly	AHP443	Pur.
CD196	4C6	MCA4623GA	Pur.
CD205	MG38	MCA2258	Pur., FITC, LE, RPE
CD206	15-2	MCA2155	Pur.
CD209	MR-1	MCA2318	Pur., A488, A647, Biot., FITC, LE, RPE
CD273	MIH14	MCA2630	Pur., A488, A647, FITC, RPE
CD274	MIH2	MCA2627	Pur., A488, A647, FITC, RPE
CD300a	MEM-260	MCA2704GA	Pur.
CD314	1D11	MCA2405	Pur., A488, A647, FITC, LE, RPE
Dectin-1	GE2	MCA4661	Pur., A488, A647, FITC, LE, RPE
Dectin-1	BD6	MCA4662	Pur., A488, A647, FITC, RPE
GITRL	Poly	AHP1865	Pur., Biot.
TSLP Receptor	Poly	AHP1431	Pur.
TSLP Receptor	4A11	MCA3918Z	Az. Fr.
小鼠树突状细胞标志物抗体
CD13	R3-63	MCA2183	Pur., A488, A647, Biot., FITC, LE, RPE
CD13	ER-BMDM1	MCA2395	Pur., A488
CD40	3/23	MCA1143	Pur., A488, A647, A700, Biot., FITC, LE,RPE, RPE- A647
CD43	1B11	MCA4702GA	Pur.
CD70	FR70	MCA2302	Pur., A488, A647, Biot., FITC
CD80	RM80	MCA2462	Pur., A488, A647, FITC, LE, RPE
CD83	3D11	MCA2747	Pur., A488, A647, FITC, RPE
CD86	RMMP-2	MCA1587	Pur., FITC
CD86	PO3	MCA2463	Pur., A488, A647, FITC, LE, RPE
CD103	2E7	MCA4705GA	Pur.
CD107b	M3/84	MCA2293	Pur., A488, A647, Biot., FITC, RPE
CD123	5B11	MCA2353	Pur., A488, A647
CD184	Poly	AAM67	Pur.
CD197	4B12	MCA2367	Pur., A488, A647, Biot., FITC, RPE
CD205	NLDC-145	MCA949	A488, A647, A700, FITC, S/N
CD206	MR5D3	MCA2235	Pur., A488, A647, Biot., FITC, LE, RPE
CD252	OX-89	MCA1881	Pur., Az. Fr.
CD273	TY25	MCA2465	Pur., A488, A647, FITC, LE, PB, RPE
CD274	MIH6	MCA2626	Pur., A488, A647, FITC, LE
Dectin-1	2A11	MCA2289	Pur., A647, Biot., FITC, LE, RPE
Dectin-2	D2.11E4	MCA2415	Pur., A488, A647, Biot., FITC, LE, RPE
Dendritic / interdigitating cells	MIDC-8	MCA948	S/N
GITRL	YGL386	MCA2417	Pur., A647, LE
大鼠树突状细胞标志物抗体
CD43	W3/13	MCA54	Pur., Biot., FITC, RPE
CD80	3H5	MCA2873	Pur., A488, A647, FITC, LE
CD86	OX-48	MCA2121	Pur., Biot., FITC, RPE
CD86	24F	MCA2874	Pur., A488, A647, FITC, LE, RPE
CD163	ED2	MCA342	Pur., A647, Biot., FITC, LE, RPE
CD200	OX-2	MCA44	Pur., Az. Fr., FITC, RPE
CD200R1	OX-102	MCA1959	Pur., Biot., FITC, LE, RPE

天然免疫学热点领域科研抗体(AbD Serotec®)—自然杀伤细胞标志物抗体

发表于2024年10月1日由上海金畔生物科技有限公司

天然免疫学热点领域科研抗体(AbD Serotec®)—自然杀伤细胞标志物抗体

自然杀伤细胞(natural killer cell，NK)是机体重要的免疫细胞，不仅与抗肿瘤、抗病毒感染和免疫调节有关，而且在某些情况下参与超敏反应和自身免疫性疾病的发生。
天然免疫学热点领域科研抗体(AbD Serotec®)---自然杀伤细胞标志物抗体

人流式抗体试剂盒4C007可用于一步法鉴定和计数T细胞，B细胞和NK细胞。CD16/CD56是抗人CD16（克隆号LNK16）和抗人CD56（克隆号MEM-188）的混合抗体。左图为使用流式抗体试剂盒4C007对人淋巴细胞流式染色结果。CD3^–(CD16/CD56)⁺为NK细胞。

人CD16又称FcγRIIIA，IgG Fc段低亲和力受体。不同人NK细胞亚群CD16表达水平不同，而且抗体依赖性细胞毒作用（antibody-dependent cellular cytotoxicity）也有差异。左图为使用mouse anti human CD16(MCA1193)对人外周血单核细胞流式染色结果。

AbD Serotec公司提供的最受欢迎的NK细胞抗体

人NK细胞抗体
产品名称		克隆号	产品编号	形式
CD3/CD16/CD56/CD45/CD19		UCHT1 / LNK16 / MEM- 188 / F10-89-4 / LT19	4C007	FITC, RPE, PERCP, APC
CD2		LT2	MCA1194	Pur., A647, Biot., FITC, LE, RPE
CD11b		ICRF44	MCA551	Pur., A647, A700, Az.Fr., Biot., FITC, RPE, RPE-A647
CD11c		Polyclonal	AHP1226	Pur.
CD16		KD	MCA5665	Pur., FITC
CD16		LNK16	MCA1193	Pur., A647, Biot., FITC, LE
CD16/CD56		LNK16/MEM-188	MCA2074	FITC
CD27		LT27	MCA755	Pur., A647, Az.Fr., FITC, RPE
CD56		123C3	MCA2693	Pur., A488, A647, A700, FITC
CD56		C5.9	MCA1437	RPE
CD56		ERIC-1	MCA591	Pur., Az.Fr.
CD56		MEM-188	MCA2046	Pur., A647, Biot., FITC
CD62L		FMC46	MCA1076	Pur., FITC, RPE
CD66acd		YTH71.3	MCA1147	Pur.
CD81		1D6	MCA1847	Pur., FITC, LE
CD85j		4F9	MCA2515	Pur., A488, A647, FITC, RPE
CD94		DX22	MCA2254	Pur., Az.Fr., Biot., FITC, RPE
CD94		HP-3B1	MCA1537	Pur.
CD95		LOB 3/11	MCA1540	Pur., Az.Fr.
CD95		LOB 3/17	MCA1539	Pur., A647, FITC, RPE
CD95			PHP192	Rec. Protein
CD96		NK92.39	MCA4643	Pur., A488, A647, FITC, LE, RPE
CD158b		190IIC311	MCA2340	Pur., A488, RPE
CD158b		GL183	MCA1584	Pur.
CD161 (KLRB1)		B199.2	MCA1855	Pur., FITC, RPE
CD244		2B4.69	MCA2421	Pur., A488, Biot., LE, RPE
CD300a		P192	MCA5747	Pur., FITC, RPE
CD314		1D11	MCA2405	Pur., A488, A647, FITC, LE, RPE
CD335		N1d9	MCA2339	Pur.
CD335		9E2	MCA4646	Pur., A488, A647, FITC, LE, RPE
CD336		3.43	MCA4665	Pur., A488, A647
GRANZYME A		GA6	MCA2117	Pur.
GRANZYME B		GB11	MCA2120	Pur.
GRANZYME B		GB7	MCA2118	Pur.
IFN Gamma		B27	MCA1554	Pur., APC, Az.Fr., Biot., FITC, RPE
IFN Gamma		D9D10	MCA1581	Pur., FITC, RPE
SIGLEC-7		S7.7	MCA5782	Pur., FITC, LE
SIGLEC-9		K8	MCA5890	Pur., FITC
SIGLEC-10		5G6	MCA5783	Pur., FITC RPE
TNF ALPHA		Polyclonal	AHP1212	Pur., Biot.
TGF beta		TB21	MCA797	Pur.
小鼠NK细胞抗体
CD2	AT37		MCA1261	Az. Fr., FITC, RPE, S/N
CD11b	5C6		MCA711	Pur., FITC, LE, RPE
CD11b	M1/70.15		MCA74	Pur., A488, A647, A700, Biot., FITC, LE, RPE, RPE-A647, RPE-A750, RPE-CY5
CD11c	N418		MCA1369	Pur., A488, A647, Biot., FITC, RPE, RPE-A647, RPE-A750
CD27	LG.3A10		MCA4701	Pur., Biot., FITC, RPE
CD45	YW62.3		MCA1031	Pur., A488, A647, A700, Biot., FITC, LE, RPE
CD45R	RA3-6B2		MCA1258	Pur., A488, A647, A700, FITC, RPE, RPE-A647, RPE-A750, RPE-CY5, RPE-CY7
CD49b	DX5		MCA2125	Pur., Biot., FITC
CD62L	MEL-14		MCA1259	Pur., Az.Fr., RPE
CD94	18d3		MCA2288	Pur., A488, A647, Biot., FITC, RPE
CD127	SB/199		MCA2306	Pur., A488, A647, FITC
CD161b/c (NK1.1)	PK136		MCA1266	Pur., Az.Fr., Biot., RPE
CD335	29A1.4		MCA5938	Pur., Biotin, FITC, LE, RPE, RPE-CY7
CLEC-2	17D9		MCA5700	Pur., FITC, RPE
IFN Gamma	XMG1.2		MCA1548	Pur., Biot., FITC, LE, RPE
NKG2A/C/E	20d5		MCA2287	Pur., A488, A647, Biot., FITC, LE, RPE

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标签归档：abd

天然免疫学热点领域科研抗体(AbD Serotec®)—报告蛋白抗体

Bevacizumab antibody | AbD17976_hIgG1

Denosumab antibody | AbD26296

犬CD分子抗体 Abd serotec公司

Bio-Rad（Abd serotec）–独特性抗体

天然免疫学热点领域科研抗体(AbD Serotec®)—巨噬细胞标志物抗体

牛CD分子抗体 Abd serotec公司

Tocilizumab antibody | AbD21338_hIgG1

天然免疫学热点领域科研抗体(AbD Serotec®)—树突状细胞标志物抗体

天然免疫学热点领域科研抗体(AbD Serotec®)—自然杀伤细胞标志物抗体

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