HLA-DOB belongs to the HLA class II beta chain paralogues.This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane.It is located in intracellular vesicles.DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM.Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages).The beta chain is approximately 26-28 kDa and its gene contains 6 exons.Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
Application
Immunology research
Derived from
E.coli
Purification
NI-NTA affinity purification
Formulation
Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0.
Shipping
The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
Storage
Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years.
Reconstitution
Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity
Purity≥85% as determined by reducing SDS-PAGE.
Endotoxin
/
Bioactive
Unknown. (Ban’t be used in biological activity experiments such as cell culture, treatment and diagnosis.)
Predicted molecular weight: 29kD Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
HLA-DPB1 抗体;HLA-DP1B; MHC class II antigen DPB1; HLA class II histocompatibility antigen
宿主
Rabbit
反应种属
Human, Mouse
应用
WB:1:1000; IHC-P:1:200-1:1000; ICC/IF:1:50;
分子量
Predicted molecular weight: 29kD Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.