基底膜是动物体内上皮细胞基底面的一层基质膜。OrganoGel 是从小鼠肿瘤组织提取的基底膜成分，所形成的基质胶。该基质胶主要成分为laminin，collagen IV，heparan sulfate proteoglycans（Kleinman et al. 1986）。同时，该基质胶也包含多种生长因子，例如表皮生长因子EFG，血小板衍生生长因子PDGF，神经生长因子NGF，碱性成纤维细胞生长因子FGF-2，乙型转化生长因子TGF-beta和胰岛素样生长因子ILGF（Vukicevic et al.1992）。
This kit is intended for use in identifying A549 Host Cell Proteins by Western Blot. The antibodies used in this kit are the same as those used in the corresponding ELISA kit, Catalog # F230.
PNPP Liquid Substrate F008 For use with Alk Phos in ELISA, 100mL
PNPP Liquid Substrate F10008 For use with Alk Phos in ELISA, 1L
TMB Liquid Substrate F027 For use with HRP in ELISA, 100mL
TMB Liquid Substrate F100027 For use with HRP in ELISA, 1L
TMB Liquid Substrate F129 For use with HRP in Western Blot, 100mL
TMB Liquid Substrate F100029 For use with HRP in Western Blot, 1L
BCIP/NBT Liquid Substrate F064 For use with Alk Phos in WB, 1 reagent, 100mL
BCIP/NBT Liquid Substrate F00064 For use with Alk Phos in WB, 1 reagent, 1L
Sample Dilution Buffers
Sample Dilution Buffer, 1L， 100mL, 500mL I028 I028-100 I028-500 For use with BHK, E.coli, CHO, CHO-CM, Human IgG's, HSA, hTransferrin, HEK 293, Insulin, L.lactic, Mouse IgG's, Pichia pastoris, S.cerevisiae, Protein A, Vero cell ELISA kits
A549 Sample Diluent, 100mL , 500mL F233A-100 F233A-500 For use with A549 HCP ELISA kit
BSA Sample Dilution Buffer, 1L , 100mL , 500mL F031A / F031A-100 / F031A-500 For use with BSA, Bovine IgG, Bovine Plasma Proteins and Bovine Transferrin ELISA kits
Goat IgG Dilution Buffer, 1L , 100mL , 500mL F213A /F213A-100 / F213A-500 For use with Goat IgG ELISA kit
Goat Milk Diluent Buffer, 1L , 100mL r, 500mL F243A /F243A-100 /F243A-500 For use with Goat Milk Proteins ELISA kit
NS/0 Sample Dilution Buffer, 1L ，100mL ，500mL F223A / F223A-100 /F223A-500 For use with NS/0 HCP ELISA kit
SP2/0 Sample Dilution Buffer, 1L , 100mL , 500mL F183A / F183A-100 / F183A-500 For use with SP2/0 HCP ELISA kit
Immunogenicity Products
Passive Coated Immunogenicity Kit IM10 Kit for immunogenicity testing
Biotinylated Antigen Coated Immunogenicity Kit IM20 Kit for immunogenicity testing
Biotinylation Kit IM30
Human Immunoglobulin Screening Kit IM40 Kit for immunogenicity testing
Human IgG Subclass Kit IM50 Kit for immunogenicity testing
Substrate Kit IM109 TMB & Stop Solution for ELISA, 50mL
Coatingate Buffer, 1L IM101 For immunogenicity testing
Conjugate Diluent, 1L IM103 For immunogenicity testing
Conjugate Diluent, 500mL IM103-500 For immunogenicity testing
Sample Diluent, 500mL IM104 For immunogenicity testing
FG Blocker, 1L IM107 For immunogenicity testing
BSA Antigen, 1mg/mL IM112 For immunogenicity testing
Bovine IgG Antigen, 1mg/mL IM113 For immunogenicity testing
Bovine Transferrin Antigen, 1mg/mL IM114 For immunogenicity testing
CHO HCP Antigen, 1mg/mL IM115 For immunogenicity testing
E. coli HCP Antigen, 1mg/mL IM116 For immunogenicity testing
Goat IgG Antigen, 1mg/mL IM117 For immunogenicity testing
HSA Antigen, 1mg/mL IM118 For immunogenicity testing
Human IgD Antigen, 1mg/mL IM141 For immunogenicity testing
Human Transferrin Antigen, 1mg/mL IM119 For immunogenicity testing
Insulin Antigen, 1mg/mL IM121 For immunogenicity testing
Mouse IgG Antigen, 1mg/mL IM122 For immunogenicity testing
NS/0 HCP Antigen, 1mg/mL IM123 For immunogenicity testing
SP2/0 HCP Antigen, 1mg/mL IM125 For immunogenicity testing
Gyros Kits-discontinued
CHO 3G HCP G550 For use on the Gyros xP workstation, 560 tests
CHO 3G HCP G550-1 For use on the Gyros xP workstation, 112 tests
E.coli HCP G410 For use on the Gyros xP workstation, 560 tests
E.coli HCP G410-1 For use on the Gyros xP workstation, 112 tests
Protein A G400 For use on the Gyros xP workstation, 560 tests
Protein A G400-1 For use on the Gyros xP workstation, 112 tests
DNA Kits
DNA Extracton in Wells D100W 96 tests in a deep well microtiter plate
DNA Extraction in Tubes D100T 50 tests in microfuge tubes
Residual CHO Host Cell kit in Wells D550W 96 tests in a deep well microtiter plate
Residual CHO Host Cell kit in Tubes D550T 50 tests in microfuge tubes
CHO Amplification kit in Wells D555W 96 tests in a deep well microtiter plate
CHO Amplification kit in Tubes D555T 50 tests in microfuge tubes
Residual Ecoli Host Cell kit in Wells D410W 96 tests in a deep well microtiter plate
Residual Ecoli Host Cell kit in Tubes D410T 50 tests in microfuge tubes
E.coli Amplification kit in Wells D415W 96 tests in a deep well microtiter plate
E.coli Amplification kit in Tubes D415T 50 tests in microfuge tubes
Residual NS/0 Host Cell kit in Wells D220W 96 tests in a deep well microtiter plate
Residual NS/0 Host Cell kit in Tubes D220T 50 tests in microfuge tubes
Miscellaneous Reagents & Components
Deep Well Extraction Plate w/Sealing Mat D102 5 plates
For determining antibiotic potency by the microbiological assay technique.
成分(g/L):
Yeast Extract/酵母浸粉
5.0
Pancreatic Digest of Casein/胰蛋白胨
9.0
Dextrose/葡萄糖
20.0
Monobasic Potassium Phosphate/磷酸二氢钾
1.0
Dibasic Potassium Phosphate/磷酸氢二钾
1.0
Sodium Chloride/氯化钠
10.0
pH值6.7 ± 0.2
25℃
用法:
Suspend 46g of the medium in one liter of distilled Water. Mix well and heat with frequent agitation until the medium is dissolved.Sterillize in an autoclave at 121℃ for 15 min. 称取本品 46g,加热溶解于 1000ml 蒸馏水或纯化水中,分装,121℃高压灭菌15分钟,备用。
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INT-767 is a potent agonist for both FXR (mean EC(50), 30 nM by PerkinElmer AlphaScreen assay) and TGR5 (mean EC(50), 630 nM by time resolved-fluorescence resonance energy transfer). INT-767 does not show cytotoxic effects in HepG2 cells. INT-767 induces FXR-dependent lipid uptake by adipocytes, with the beneficial effect of shuttling lipids from central hepatic to peripheral fat storage, and promotes TGR5-dependent glucagon-like peptide-1 secretion by enteroendocrine cells, a validated target in the treatment of type 2 diabetes. [2]
In Vivo
Only the dual FXR/TGR5 agonist, INT-767, significantly improved serum liver enzymes, hepatic inflammation, and biliary fibrosis in Mdr2(-/-) mice. In line with this, INT-767 significantly induced bile flow and biliary HCO 3- output, as well as gene expression of carbonic anhydrase 14, an important enzyme able to enhance HCO 3- transport, in an Fxr-dependent manner. In addition, INT-767 dramatically reduced bile acid synthesis via the induction of ileal Fgf15 and hepatic Shp gene expression, thus resulting in significantly reduced biliary bile acid output in Mdr2(-/-) mice.[1] INT-767 treatment markedly decreases cholesterol and triglyceride levels in diabetic db/db mice and in mice rendered diabetic by streptozotocin administration. [2]
[1]. Baghdasaryan A, et al. Dual farnesoid X receptor/TGR5 agonist INT-767 reduces liver injury in the Mdr2-/- (Abcb4-/-) mousecholangiopathy model by promoting biliary HCO3- output. Hepatology. 2011 Oct;54(4):1303-1312. [2]. Rizzo G, et al. Functional characterization of the semisynthetic bile acid derivative INT-767, a dual farnesoid X receptor andTGR5 agonist. Mol Pharmacol. 2010 Oct;78(4):617-630.
GE WHATMAN 6781-2502聚醚砜Puradisc 25mm针头式滤器200pk，Puradisc针头式滤器具有*的质量，是Z经济的选择。它可以快速有效的过滤样品，Z大过滤量为100ml。 Puradisc滤器由无色聚丙烯或聚碳酸酯材料制成，有标准进口和出口接头（除非另有说明）。有无菌包装、医疗级别包装可选，还有特殊管出口可用于高精确度样品分离回收入微型管，避免了空气阻塞作用。
【详细说明】
GE WHATMAN 6781-2502聚醚砜Puradisc 25mm针头式滤器200pk
GE WHATMAN 6781-2502聚醚砜Puradisc 25mm针头式滤器200pk，Puradisc针头式滤器具有*的质量，是的选择。它可以快速有效的过滤样品，zui大过滤量为100ml。
