将细胞在96孔板中暴露于各种浓度的罗米地辛72小时。向每个孔中加入20μL5mg/ mL MTT的PBS溶液4小时。除去培养基后,向各孔中加入170μLDMSO以溶解甲crystals晶体。测定540nm处的吸光度。另外,将细胞与台盼蓝一起孵育,并在血细胞计数器中计数蓝色(死亡)细胞和透明(活)细胞的数量。对于细胞周期分析,将细胞在含有0.05mg/mL碘化丙啶,1mM EDTA,0.1%Triton X-100和PBS中1mg/mL RNase A的碘化丙锭染色溶液中孵育30分钟。然后使悬浮液通过尼龙筛网过滤器并在Becton Dickinson FACScan上分析。
数据来源文献
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